The molecular structure of a DNA-triostin A complex

Andrew H.J. Wang, Giovanni Ughetto, Gary J. Quigley, Toshio Hakoshima, Gijs A. Van Der Marel, Jacques H. Van Boom, Alexander Rich

Research output: Contribution to journalArticlepeer-review

249 Citations (Scopus)

Abstract

The molecular structure of triostin A, a cyclic octadepsipeptide antibiotic, has been solved complexed to a DNA double helical fragment with the sequence CGTACG (C, cytosine; G, guanine; T, thymine; A, adenine). The two planar quinoxaline rings of triostin A bis intercalate on the minor groove of the DNA double helix surrounding the CG base pairs at either end. The alanine residues form hydrogen bonds to the guanines. Base stacking in the DNA is perturbed, and the major binding interaction involves a large number of van der Waals contacts between the peptides and the nucleic acid. The adenine residues in the center are in the syn conformation and are paired to thymine through Hoogsteen base pairing.

Original languageEnglish
Pages (from-to)1115-1121
Number of pages7
JournalScience
Volume225
Issue number4667
DOIs
Publication statusPublished - 1984
Externally publishedYes

ASJC Scopus subject areas

  • General

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