The molecular mechanism of actinomycin D in preventing neointimal formation in rat carotid arteries after balloon injury

C. H. Wu, J. S. Pan, W. C. Chang, J. S. Hung, Simon J T Mao

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The pathological mechanism of restenosis is primarily attributed to excessive proliferation of vascular smooth muscle cells (SMC). Actinomycin D has been regarded as a potential candidate to prevent balloon injury-induced neointimal formation. To explore its molecular mechanism in regulating cell proliferation, we first showed that actinomycin D markedly reduced the SMC proliferation via the inhibition of BrdU incorporation at 80 nM. This was further supported by the G1-phase arrest using a flowcytometric analysis. Actinomycin D was extremely potent with an inhibitory concentration IC 50 at 0.4 nM, whereas the lethal dose LD50 was at 260 μM. In an in vivo study, the pluronic gel containing 80 nM and 80 μM actinomycin D was applied topically to surround the rat carotid adventitia; the thickness of neointima was substantially reduced (45 and 55%, respectively). The protein expression levels of proliferating cell nuclear antigen (PCNA), focal adhesion kinase (FAK), and Raf were all suppressed by actinomycin D. Extracellular signal-regulated kinases (Erk) involved in cell-cycle arrest were found to increase by actinomycin D. These observations provide a detailed mechanism of actinomycin D in preventing cell proliferation thus as a potential intervention for restenosis.

Original languageEnglish
Pages (from-to)503-512
Number of pages10
JournalJournal of Biomedical Science
Volume12
Issue number3
DOIs
Publication statusPublished - May 2005
Externally publishedYes

Fingerprint

Balloons
Dactinomycin
Carotid Arteries
Rats
Wounds and Injuries
Cell proliferation
Cell Proliferation
Smooth Muscle Myocytes
Muscle
Cells
Poloxamer
Neointima
Focal Adhesion Protein-Tyrosine Kinases
Adventitia
Lethal Dose 50
Extracellular Signal-Regulated MAP Kinases
Proliferating Cell Nuclear Antigen
G1 Phase
Bromodeoxyuridine
Cell Cycle Checkpoints

Keywords

  • Actinomycin D
  • Neointimal formation
  • Proliferation
  • Restenosis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The molecular mechanism of actinomycin D in preventing neointimal formation in rat carotid arteries after balloon injury. / Wu, C. H.; Pan, J. S.; Chang, W. C.; Hung, J. S.; Mao, Simon J T.

In: Journal of Biomedical Science, Vol. 12, No. 3, 05.2005, p. 503-512.

Research output: Contribution to journalArticle

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