The metabolome profiling and pathway analysis in metabolic healthy and abnormal obesity

H. H. Chen, Y. J. Tseng, S. Y. Wang, Y. S. Tsai, C. S. Chang, T. C. Kuo, W. J. Yao, C. C. Shieh, C. H. Wu, P. H. Kuo

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Objectives:Mechanisms of the development of abnormal metabolic phenotypes among obese population are not yet clear. In this study, we aimed to screen metabolomes of both healthy and subjects with abnormal obesity to identify potential metabolic pathways that may regulate the different metabolic characteristics of obesity.Methods:We recruited subjects with body mass index (BMI) over 25 from the weight-loss clinic of a central hospital in Taiwan. Metabolic healthy obesity (MHO) is defined as without having any form of hyperglycemia, hypertension and dyslipidemia, while metabolic abnormal obesity (MAO) is defined as having one or more abnormal metabolic indexes. Serum-based metabolomic profiling using both liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry of 34 MHO and MAO individuals with matching age, sex and BMI was performed. Conditional logistic regression and partial least squares discriminant analysis were applied to identify significant metabolites between the two groups. Pathway enrichment and topology analyses were conducted to evaluate the regulated pathways.Results:A differential metabolite panel was identified to be significantly differed in MHO and MAO groups, including L-kynurenine, glycerophosphocholine (GPC), glycerol 1-phosphate, glycolic acid, tagatose, methyl palmitate and uric acid. Moreover, several metabolic pathways were relevant in distinguishing MHO from MAO groups, including fatty acid biosynthesis, phenylalanine metabolism, propanoate metabolism, and valine, leucine and isoleucine degradation.Conclusion:Different metabolomic profiles and metabolic pathways are important for distinguishing between MHO and MAO groups. We have identified and discussed the key metabolites and pathways that may prove important in the regulation of metabolic traits among the obese, which could provide useful clues to study the underlying mechanisms of the development of abnormal metabolic phenotypes.

Original languageEnglish
Pages (from-to)1241-1248
Number of pages8
JournalInternational Journal of Obesity
Volume39
Issue number8
DOIs
Publication statusPublished - Aug 6 2015
Externally publishedYes

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Metabolome
Obesity
Metabolic Networks and Pathways
Metabolomics
glycolic acid
Gas Chromatography-Mass Spectrometry
Body Mass Index
Kynurenine
Phenotype
Isoleucine
Propionates
Valine
Discriminant Analysis
Dyslipidemias
Uric Acid
Least-Squares Analysis
Phenylalanine
Taiwan
Leucine
Liquid Chromatography

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

The metabolome profiling and pathway analysis in metabolic healthy and abnormal obesity. / Chen, H. H.; Tseng, Y. J.; Wang, S. Y.; Tsai, Y. S.; Chang, C. S.; Kuo, T. C.; Yao, W. J.; Shieh, C. C.; Wu, C. H.; Kuo, P. H.

In: International Journal of Obesity, Vol. 39, No. 8, 06.08.2015, p. 1241-1248.

Research output: Contribution to journalArticle

Chen, HH, Tseng, YJ, Wang, SY, Tsai, YS, Chang, CS, Kuo, TC, Yao, WJ, Shieh, CC, Wu, CH & Kuo, PH 2015, 'The metabolome profiling and pathway analysis in metabolic healthy and abnormal obesity', International Journal of Obesity, vol. 39, no. 8, pp. 1241-1248. https://doi.org/10.1038/ijo.2015.65
Chen, H. H. ; Tseng, Y. J. ; Wang, S. Y. ; Tsai, Y. S. ; Chang, C. S. ; Kuo, T. C. ; Yao, W. J. ; Shieh, C. C. ; Wu, C. H. ; Kuo, P. H. / The metabolome profiling and pathway analysis in metabolic healthy and abnormal obesity. In: International Journal of Obesity. 2015 ; Vol. 39, No. 8. pp. 1241-1248.
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AU - Chang, C. S.

AU - Kuo, T. C.

AU - Yao, W. J.

AU - Shieh, C. C.

AU - Wu, C. H.

AU - Kuo, P. H.

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