The inhibitory effect of trilinolein on norepinephrine-induced β-myosin heavy chain promoter activity, reactive oxygen species generation, and extracellular signal-regulated kinase phosphorylation in neonatal rat cardiomyocytes

Ju Chi Liu, Paul Chan, Jin Jer Chen, Horng Mo Lee, Wen Sen Lee, Neng Lang Shih, Yen Ling Chen, Hong Jye Hong, Tzu-Hurng Cheng

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Abstract

The myocardial protective effects of trilinolein, isolated from the traditional Chinese herb Sanchi (Panax notoginseng),are thought to be related to its antioxidant activity. However, the intracellular mechanism underlying the protective effect of trilinolein in the heart remains unclear. In the present study, we investigated the effect of trilinolein on norepinephrine (NE)-induced protein synthesis in cardiomyocytes. Cultured neonatal rat cardiomyocytes were stimulated with NE, then protein content, [ 3H]-leucine incorporation, and β-myosin heavy chain (β-MyHC) promoter activity were examined. The effect of trilinolein on NE-induced intracellular reactive oxygen species (ROS) generation was measured with a redox-sensitive fluorescent dye (2′,7′-dichlorofluorescin diacetate) and extracellular signal-regulated kinase (ERK) phosphorylation by Western blotting. Trilinolein inhibited NE-increased protein synthesis, β-MyHC promoter activity, and intracellular ROS. Both trilinolein and the antioxidant, N-acetyl-cysteine, decreased NE- and H2O 2-induced protein synthesis, β-MyHC promoter activity, and ERK phosphorylation. These data indicate that trilinolein inhibits NE-induced protein synthesis via attenuation of ROS generation in cardiomyocytes.

Original languageEnglish
Pages (from-to)11-18
Number of pages8
JournalJournal of Biomedical Science
Volume11
Issue number1
DOIs
Publication statusPublished - 2004

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Phosphorylation
Myosin Heavy Chains
Extracellular Signal-Regulated MAP Kinases
Cardiac Myocytes
Rats
Reactive Oxygen Species
Norepinephrine
Proteins
Antioxidants
Panax notoginseng
Acetylcysteine
trilinolein
Fluorescent Dyes
Leucine
Oxidation-Reduction
Cysteine
Western Blotting

Keywords

  • β-Myosin heavy chain gene
  • Extracellular signal-regulated kinase
  • Norepinephrine
  • Reactive oxygen species
  • Trilinolein

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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title = "The inhibitory effect of trilinolein on norepinephrine-induced β-myosin heavy chain promoter activity, reactive oxygen species generation, and extracellular signal-regulated kinase phosphorylation in neonatal rat cardiomyocytes",
abstract = "The myocardial protective effects of trilinolein, isolated from the traditional Chinese herb Sanchi (Panax notoginseng),are thought to be related to its antioxidant activity. However, the intracellular mechanism underlying the protective effect of trilinolein in the heart remains unclear. In the present study, we investigated the effect of trilinolein on norepinephrine (NE)-induced protein synthesis in cardiomyocytes. Cultured neonatal rat cardiomyocytes were stimulated with NE, then protein content, [ 3H]-leucine incorporation, and β-myosin heavy chain (β-MyHC) promoter activity were examined. The effect of trilinolein on NE-induced intracellular reactive oxygen species (ROS) generation was measured with a redox-sensitive fluorescent dye (2′,7′-dichlorofluorescin diacetate) and extracellular signal-regulated kinase (ERK) phosphorylation by Western blotting. Trilinolein inhibited NE-increased protein synthesis, β-MyHC promoter activity, and intracellular ROS. Both trilinolein and the antioxidant, N-acetyl-cysteine, decreased NE- and H2O 2-induced protein synthesis, β-MyHC promoter activity, and ERK phosphorylation. These data indicate that trilinolein inhibits NE-induced protein synthesis via attenuation of ROS generation in cardiomyocytes.",
keywords = "β-Myosin heavy chain gene, Extracellular signal-regulated kinase, Norepinephrine, Reactive oxygen species, Trilinolein",
author = "Liu, {Ju Chi} and Paul Chan and Chen, {Jin Jer} and Lee, {Horng Mo} and Lee, {Wen Sen} and Shih, {Neng Lang} and Chen, {Yen Ling} and Hong, {Hong Jye} and Tzu-Hurng Cheng",
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T1 - The inhibitory effect of trilinolein on norepinephrine-induced β-myosin heavy chain promoter activity, reactive oxygen species generation, and extracellular signal-regulated kinase phosphorylation in neonatal rat cardiomyocytes

AU - Liu, Ju Chi

AU - Chan, Paul

AU - Chen, Jin Jer

AU - Lee, Horng Mo

AU - Lee, Wen Sen

AU - Shih, Neng Lang

AU - Chen, Yen Ling

AU - Hong, Hong Jye

AU - Cheng, Tzu-Hurng

PY - 2004

Y1 - 2004

N2 - The myocardial protective effects of trilinolein, isolated from the traditional Chinese herb Sanchi (Panax notoginseng),are thought to be related to its antioxidant activity. However, the intracellular mechanism underlying the protective effect of trilinolein in the heart remains unclear. In the present study, we investigated the effect of trilinolein on norepinephrine (NE)-induced protein synthesis in cardiomyocytes. Cultured neonatal rat cardiomyocytes were stimulated with NE, then protein content, [ 3H]-leucine incorporation, and β-myosin heavy chain (β-MyHC) promoter activity were examined. The effect of trilinolein on NE-induced intracellular reactive oxygen species (ROS) generation was measured with a redox-sensitive fluorescent dye (2′,7′-dichlorofluorescin diacetate) and extracellular signal-regulated kinase (ERK) phosphorylation by Western blotting. Trilinolein inhibited NE-increased protein synthesis, β-MyHC promoter activity, and intracellular ROS. Both trilinolein and the antioxidant, N-acetyl-cysteine, decreased NE- and H2O 2-induced protein synthesis, β-MyHC promoter activity, and ERK phosphorylation. These data indicate that trilinolein inhibits NE-induced protein synthesis via attenuation of ROS generation in cardiomyocytes.

AB - The myocardial protective effects of trilinolein, isolated from the traditional Chinese herb Sanchi (Panax notoginseng),are thought to be related to its antioxidant activity. However, the intracellular mechanism underlying the protective effect of trilinolein in the heart remains unclear. In the present study, we investigated the effect of trilinolein on norepinephrine (NE)-induced protein synthesis in cardiomyocytes. Cultured neonatal rat cardiomyocytes were stimulated with NE, then protein content, [ 3H]-leucine incorporation, and β-myosin heavy chain (β-MyHC) promoter activity were examined. The effect of trilinolein on NE-induced intracellular reactive oxygen species (ROS) generation was measured with a redox-sensitive fluorescent dye (2′,7′-dichlorofluorescin diacetate) and extracellular signal-regulated kinase (ERK) phosphorylation by Western blotting. Trilinolein inhibited NE-increased protein synthesis, β-MyHC promoter activity, and intracellular ROS. Both trilinolein and the antioxidant, N-acetyl-cysteine, decreased NE- and H2O 2-induced protein synthesis, β-MyHC promoter activity, and ERK phosphorylation. These data indicate that trilinolein inhibits NE-induced protein synthesis via attenuation of ROS generation in cardiomyocytes.

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