The inhibitory effect of trilinolein on norepinephrine-induced β-myosin heavy chain promoter activity, reactive oxygen species generation, and extracellular signal-regulated kinase phosphorylation in neonatal rat cardiomyocytes

Ju Chi Liu, Paul Chan, Jin Jer Chen, Horng Mo Lee, Wen Sen Lee, Neng Lang Shih, Yen Ling Chen, Hong Jye Hong, Tzu-Hurng Cheng

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Abstract

The myocardial protective effects of trilinolein, isolated from the traditional Chinese herb Sanchi (Panax notoginseng),are thought to be related to its antioxidant activity. However, the intracellular mechanism underlying the protective effect of trilinolein in the heart remains unclear. In the present study, we investigated the effect of trilinolein on norepinephrine (NE)-induced protein synthesis in cardiomyocytes. Cultured neonatal rat cardiomyocytes were stimulated with NE, then protein content, [ 3H]-leucine incorporation, and β-myosin heavy chain (β-MyHC) promoter activity were examined. The effect of trilinolein on NE-induced intracellular reactive oxygen species (ROS) generation was measured with a redox-sensitive fluorescent dye (2′,7′-dichlorofluorescin diacetate) and extracellular signal-regulated kinase (ERK) phosphorylation by Western blotting. Trilinolein inhibited NE-increased protein synthesis, β-MyHC promoter activity, and intracellular ROS. Both trilinolein and the antioxidant, N-acetyl-cysteine, decreased NE- and H2O 2-induced protein synthesis, β-MyHC promoter activity, and ERK phosphorylation. These data indicate that trilinolein inhibits NE-induced protein synthesis via attenuation of ROS generation in cardiomyocytes.

Original languageEnglish
Pages (from-to)11-18
Number of pages8
JournalJournal of Biomedical Science
Volume11
Issue number1
DOIs
Publication statusPublished - 2004

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Keywords

  • β-Myosin heavy chain gene
  • Extracellular signal-regulated kinase
  • Norepinephrine
  • Reactive oxygen species
  • Trilinolein

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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