The inhibition of lung cancer cell migration by AhR-regulated autophagy

Chi Hao Tsai, Ching Hao Li, Yu Wen Cheng, Chen Chen Lee, Po Lin Liao, Cheng Hui Lin, Shih Hsuan Huang, Jaw Jou Kang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that is highly expressed in multiple organs and tissues. Whereas AhR mediates the metabolism of xenobiotic and endogenous compounds, its novel function in cancer epithelial-mesenchymal transition (EMT) remains controversial. Autophagy also participates in tumour progression through its functions in cell homeostasis and facilitates adaptation to EMT progression. In the present study, we found that AhR-regulated autophagy positively modulates EMT in non-small cell lung cancer cells. The motility of A549, H1299, and CL1-5 cells were correlated with different AhR expression levels. Invasive potential and cell morphology also changed when AhR protein expression was altered. Moreover, AhR levels exerted a contrasting effect on autophagy potential. Autophagy was higher in CL1-5 and H1299 cells with lower AhR levels than in A549 cells. Both AhR overexpression and autophagy inhibition decreased CL1-5 metastasis in vivo. Furthermore, AhR promoted BNIP3 ubiquitination for proteasomal degradation. AhR silencing in A549 cells also reduced BNIP3 ubiquitination. Taken together, these results provide a novel insight into the cross-linking between AhR and autophagy, we addressed the mechanistic BNIP3 modulation by endogenous AhR, which affect cancer cell EMT progression.

Original languageEnglish
Article number41927
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - Feb 14 2017

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Aryl Hydrocarbon Receptors
Autophagy
Cell Movement
Lung Neoplasms
Epithelial-Mesenchymal Transition
Ubiquitination
Neoplasms
Xenobiotics
Non-Small Cell Lung Carcinoma
Homeostasis
Transcription Factors

ASJC Scopus subject areas

  • General

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The inhibition of lung cancer cell migration by AhR-regulated autophagy. / Tsai, Chi Hao; Li, Ching Hao; Cheng, Yu Wen; Lee, Chen Chen; Liao, Po Lin; Lin, Cheng Hui; Huang, Shih Hsuan; Kang, Jaw Jou.

In: Scientific Reports, Vol. 7, 41927, 14.02.2017.

Research output: Contribution to journalArticle

Tsai, Chi Hao ; Li, Ching Hao ; Cheng, Yu Wen ; Lee, Chen Chen ; Liao, Po Lin ; Lin, Cheng Hui ; Huang, Shih Hsuan ; Kang, Jaw Jou. / The inhibition of lung cancer cell migration by AhR-regulated autophagy. In: Scientific Reports. 2017 ; Vol. 7.
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