The inhibition of cordycepin on cancer stemness in TGF-beta induced chemo-resistant ovarian cancer cell

Chia Woei Wang, Bao Hong Lee, Chen Jei Tai

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Chemotherapy is one of the main approach for ovarian cancer. Cancer stem cells (CSCs) escape chemotherapy and lead to chemoresistance. We previously demonstrated that cordycepin (Cd) inhibited metastasis in human ovarian carcinoma cells, the aim of this study is to investigate the effects of Cd on ovarian cancer stemness. TGF-beta was used to induce chemoresistance of chemotherapeutic agent cisplatin in SKOV-3 ovarian cancer cells. After treating with 100 μM of Cd, cell viability, the percentage of cancer stem cells, and the levels of matrix metalloproteinases (MMPs) were decreased in TGF-beta-induced SKOV-3 cells. Treatment of Cd recovered E-cadherin levels and inhibited vimentin levels while TGF-beta treatment significantly increased the expression of vimentin and PGC-1alpha, and decreased E-cadherin levels in SKOV-3 cells, indicating that the action of Cd on cancer stemness may contribute to the regulation of epithelial-mesenchymal transition (EMT). Cd efficiently attenuated chemoresistance caused by TGF-beta in SKOV-3 cancer stem cells to promote the cytotoxicity of cisplatin.

Original languageEnglish
Pages (from-to)111912-111921
Number of pages10
JournalOncotarget
Volume8
Issue number67
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Transforming Growth Factor beta
Ovarian Neoplasms
Neoplastic Stem Cells
Neoplasms
Vimentin
Cadherins
Cisplatin
Drug Therapy
Epithelial-Mesenchymal Transition
Matrix Metalloproteinases
cordycepin
Cell Survival
Neoplasm Metastasis
Carcinoma
Therapeutics

Keywords

  • Cancer stem cells
  • Chemoresistance
  • Chemotherapy
  • Cordycepin
  • Ovarian cancer

ASJC Scopus subject areas

  • Oncology

Cite this

The inhibition of cordycepin on cancer stemness in TGF-beta induced chemo-resistant ovarian cancer cell. / Wang, Chia Woei; Lee, Bao Hong; Tai, Chen Jei.

In: Oncotarget, Vol. 8, No. 67, 01.01.2017, p. 111912-111921.

Research output: Contribution to journalArticle

@article{9b524b117caa4377abc5f07efddd64c8,
title = "The inhibition of cordycepin on cancer stemness in TGF-beta induced chemo-resistant ovarian cancer cell",
abstract = "Chemotherapy is one of the main approach for ovarian cancer. Cancer stem cells (CSCs) escape chemotherapy and lead to chemoresistance. We previously demonstrated that cordycepin (Cd) inhibited metastasis in human ovarian carcinoma cells, the aim of this study is to investigate the effects of Cd on ovarian cancer stemness. TGF-beta was used to induce chemoresistance of chemotherapeutic agent cisplatin in SKOV-3 ovarian cancer cells. After treating with 100 μM of Cd, cell viability, the percentage of cancer stem cells, and the levels of matrix metalloproteinases (MMPs) were decreased in TGF-beta-induced SKOV-3 cells. Treatment of Cd recovered E-cadherin levels and inhibited vimentin levels while TGF-beta treatment significantly increased the expression of vimentin and PGC-1alpha, and decreased E-cadherin levels in SKOV-3 cells, indicating that the action of Cd on cancer stemness may contribute to the regulation of epithelial-mesenchymal transition (EMT). Cd efficiently attenuated chemoresistance caused by TGF-beta in SKOV-3 cancer stem cells to promote the cytotoxicity of cisplatin.",
keywords = "Cancer stem cells, Chemoresistance, Chemotherapy, Cordycepin, Ovarian cancer",
author = "Wang, {Chia Woei} and Lee, {Bao Hong} and Tai, {Chen Jei}",
year = "2017",
month = "1",
day = "1",
doi = "10.18632/oncotarget.22951",
language = "English",
volume = "8",
pages = "111912--111921",
journal = "Oncotarget",
issn = "1949-2553",
publisher = "Impact Journals LLC",
number = "67",

}

TY - JOUR

T1 - The inhibition of cordycepin on cancer stemness in TGF-beta induced chemo-resistant ovarian cancer cell

AU - Wang, Chia Woei

AU - Lee, Bao Hong

AU - Tai, Chen Jei

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Chemotherapy is one of the main approach for ovarian cancer. Cancer stem cells (CSCs) escape chemotherapy and lead to chemoresistance. We previously demonstrated that cordycepin (Cd) inhibited metastasis in human ovarian carcinoma cells, the aim of this study is to investigate the effects of Cd on ovarian cancer stemness. TGF-beta was used to induce chemoresistance of chemotherapeutic agent cisplatin in SKOV-3 ovarian cancer cells. After treating with 100 μM of Cd, cell viability, the percentage of cancer stem cells, and the levels of matrix metalloproteinases (MMPs) were decreased in TGF-beta-induced SKOV-3 cells. Treatment of Cd recovered E-cadherin levels and inhibited vimentin levels while TGF-beta treatment significantly increased the expression of vimentin and PGC-1alpha, and decreased E-cadherin levels in SKOV-3 cells, indicating that the action of Cd on cancer stemness may contribute to the regulation of epithelial-mesenchymal transition (EMT). Cd efficiently attenuated chemoresistance caused by TGF-beta in SKOV-3 cancer stem cells to promote the cytotoxicity of cisplatin.

AB - Chemotherapy is one of the main approach for ovarian cancer. Cancer stem cells (CSCs) escape chemotherapy and lead to chemoresistance. We previously demonstrated that cordycepin (Cd) inhibited metastasis in human ovarian carcinoma cells, the aim of this study is to investigate the effects of Cd on ovarian cancer stemness. TGF-beta was used to induce chemoresistance of chemotherapeutic agent cisplatin in SKOV-3 ovarian cancer cells. After treating with 100 μM of Cd, cell viability, the percentage of cancer stem cells, and the levels of matrix metalloproteinases (MMPs) were decreased in TGF-beta-induced SKOV-3 cells. Treatment of Cd recovered E-cadherin levels and inhibited vimentin levels while TGF-beta treatment significantly increased the expression of vimentin and PGC-1alpha, and decreased E-cadherin levels in SKOV-3 cells, indicating that the action of Cd on cancer stemness may contribute to the regulation of epithelial-mesenchymal transition (EMT). Cd efficiently attenuated chemoresistance caused by TGF-beta in SKOV-3 cancer stem cells to promote the cytotoxicity of cisplatin.

KW - Cancer stem cells

KW - Chemoresistance

KW - Chemotherapy

KW - Cordycepin

KW - Ovarian cancer

UR - http://www.scopus.com/inward/record.url?scp=85038440077&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85038440077&partnerID=8YFLogxK

U2 - 10.18632/oncotarget.22951

DO - 10.18632/oncotarget.22951

M3 - Article

AN - SCOPUS:85038440077

VL - 8

SP - 111912

EP - 111921

JO - Oncotarget

JF - Oncotarget

SN - 1949-2553

IS - 67

ER -