The influence of vancomycin on extracellular matrix and pro-inflammatory cytokine expression in human articular chondrocytes

Yi Ru Chen, Jui Hung Chang, Kai Chiang Yang, Hsein Kun Lu, Fedor Svyatoslavovich Senatov, Chang Chin Wu, Mong Hsun Tsai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Intravenous infusion or intra-articular administration of vancomycin is widely used to treat septic arthritis. However, the cytotoxic effects of vancomycin at cellular and molecular levels have yet to be fully elucidated in chondrocytes. Primary human chondrocytes were cultured in media supplemented with vancomycin at a series of concentrations (0, 100, 500, 1000, and 5000μg/mL) to study the dose-dependent toxicity. Since inflammatory chondrocytes are more catabolic than normal cells, the influence of vancomycin (500μg/mL) on interleukin-1beta (IL-1β)-stimulated chondrocytes was evaluated. Exposure of chondrocytes to a high dose of vancomycin (≥1000μg/mL) resulted in chondrotoxicity. Non-lethal dose of vancomycin (500μg/mL) did not change the viability, morphology or glycosaminoglycan content of the normal or IL-1β-stimulated chondrocytes, or affect the mRNA levels of extracellular matrixes (aggrecan, collagen type I, II, and X) and pro-inflammatory cytokines (IL-1β, TNF-α, IL-6, IL-10, MMP-3, MMP-9, and TIMP-1). ELISA revealed that vancomycin exposure did not affect the levels of PGE2, IL-1β, MMP-3, or MMP-9 in normal and IL-1β-stimulated cells. Western blotting further showed that vancomycin did not affect IκB and p50 to initiate the NF-κB pathway or COX-2 synthesis. Vancomycin at a low dose (500μg/mL) did not have a deleterious effect on chondrocytes.

Original languageEnglish
JournalProcess Biochemistry
DOIs
Publication statusPublished - 2018

Fingerprint

Vancomycin
Chondrocytes
Collagen
Extracellular Matrix
Toxicity
Joints
Cytokines
Interleukin-1beta
Matrix Metalloproteinases
Collagen Type X
Far-Western Blotting
Glycosaminoglycans
Messenger RNA
Aggrecans
Infectious Arthritis
Tissue Inhibitor of Metalloproteinase-1
Collagen Type II
Collagen Type I
Dinoprostone
Intravenous Infusions

Keywords

  • Antibiotic management
  • Chondrocyte
  • Septic arthritis
  • Vancomycin

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

The influence of vancomycin on extracellular matrix and pro-inflammatory cytokine expression in human articular chondrocytes. / Chen, Yi Ru; Chang, Jui Hung; Yang, Kai Chiang; Lu, Hsein Kun; Senatov, Fedor Svyatoslavovich; Wu, Chang Chin; Tsai, Mong Hsun.

In: Process Biochemistry, 2018.

Research output: Contribution to journalArticle

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