The influence of propolis ethanol extract on liver microsomal enzymes and glutathione after chronic alcohol administration

Song Chow Lin, Chia Yu Chung, Chia Lien Chiang, Shih Hsien Hsu

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Propolis designates a series of gums, resins and balms of viscous consistency, which are gathered by honeybees from certain parts, mainly the buds and barks of plants, especially those found on coniferous trees. Bees bring propolis back to the hive, where it is modified and mixed with other substances including the bees' own wax and salivary secretions. In this study, the influences of propolis ethanol extract on chronic alcohol induced liver microsomal enzyme changes were investigated. Three grams of alcohol was added to rats' daily diet for four weeks to induce chronic alcohol liver injuries, and two different doses of propolis ethanol extract were p.o. administrated three times per day on the 28th, 29th, and 30th day. During the period of propolis administration, the ethanol diet was continued. After sacrifice, the rat livers were excised for assay of microsomal enzymes activity, glutathione (GSH) concentration, glutathione-S-transferase (GSTase) and y-glutamylcysteine synthetase (y-GCSase) activity. It was found that 30 mg/kg of propolis ethanol extract significantly prevented the elevations of total cytochrome P-450 enzymes, NADPH-dependent cytochrome C reductase, aniline hydroxylation, 7-ethoxyresorufin hydroxylation (7-ERH), 7-penthoxyresorufin hydroxylation (7-PRH), and lipid peroxidation induced by chronic ethanol administration. Additionally, propolis ethanol extract (100 mg/kg) also induced GSTase and y-GCSase activities and decreased glutathione levels in the liver.

Original languageEnglish
Pages (from-to)83-93
Number of pages11
JournalAmerican Journal of Chinese Medicine
Volume27
Issue number1
Publication statusPublished - 1999

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Propolis
Liver Extracts
Glutathione
Ethanol
Alcohols
Enzymes
Hydroxylation
Glutamate-Cysteine Ligase
Bees
Liver
Glutathione Transferase
Plant Bark
Cytochrome Reductases
Diet
Waxes
Urticaria
Enzyme Assays
Gingiva
NADP
Cytochrome P-450 Enzyme System

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

The influence of propolis ethanol extract on liver microsomal enzymes and glutathione after chronic alcohol administration. / Lin, Song Chow; Chung, Chia Yu; Chiang, Chia Lien; Hsu, Shih Hsien.

In: American Journal of Chinese Medicine, Vol. 27, No. 1, 1999, p. 83-93.

Research output: Contribution to journalArticle

Lin, Song Chow ; Chung, Chia Yu ; Chiang, Chia Lien ; Hsu, Shih Hsien. / The influence of propolis ethanol extract on liver microsomal enzymes and glutathione after chronic alcohol administration. In: American Journal of Chinese Medicine. 1999 ; Vol. 27, No. 1. pp. 83-93.
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