The in vivo performance of polyvinyl alcohol macro-encapsulated islets

Zhi Qi, Yanna Shen, Goichi Yanai, Kaichiang Yang, Yasumasa Shirouzu, Akihito Hiura, Shoichiro Sumi

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Islet transplantation is a method for the treatment of type 1 diabetes mellitus (DM) and has been widely performed around the world. The long-term cryopreservation of islets shows many advantages in the field of islet transplantation. Previous studies have described the development of sheet-type polyvinyl alcohol (PVA) macro-encapsulated islets (MEI) to treat type 1 DM without any immunotherapy. The present study examined their beneficial effects on islet cryopreservation. PVA MEI of Wistar rats were divided into three groups of 1-day, 7-day and 30-day cryopreservation at -80 °C. The 30-day group showed a lower recovery rate of the islet number and impaired insulin release in comparison to the 1-day group, whereas no significant differences of the in vitro results were observed between the 1-day and 7-day groups. The MEI transplantation recipient mice in the 1-day and 7-day groups reached normoglycemia for a 4-week observation period, and the recipients in 30-day group also showed a significant decrease followed by a slightly higher non-fasting blood glucose level. These results suggest that the PVA MEI are useful for islet long-term cryopreservation, and that the use of cryopreserved PVA MEI may, therefore, be a promising modality for performing DM therapy.

Original languageEnglish
Pages (from-to)4026-4031
Number of pages6
JournalBiomaterials
Volume31
Issue number14
DOIs
Publication statusPublished - May 2010
Externally publishedYes

Keywords

  • Islet cryopreservation
  • Islet transplantation
  • Macro-encapsulated islets (MEIs)
  • Polyvinyl alcohol (PVA)

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

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