The in vivo distribution of murine lymphokine activated killer cells in splenectomized host.

T. Y. Chao, T. M. Chu

Research output: Contribution to journalArticle

Abstract

The in vivo distribution of intravenously injected lymphokine activated killer (LAK) cells, generated in vitro with rIL-2 from normal murine splenocytes, was studied in BALB/c mice and compared with that of normal splenocytes. Both normal splenocytes and LAK cells were labeled with 51Cr, and the results were analyzed at 6, 24, and 48 hours after injection by localization index as the parameter. After injection through tail veins of mice, LAK cells were found to migrate to the spleen, lungs, liver, lymph nodes, bones and the kidneys. The apparent increased distribution pattern of LAK cells to the lung at 6 and 24 hours after injection was not detected when normal splenocytes were injected. Since almost one third of the injected LAK cells were found to localize in the spleen, it was postulated that splenectomy would affect the in vivo organ distribution of LAK cells. Accordingly, the in vivo distribution of LAK cells in splenectomized mice was further investigated. Results indicated that splenectomy enhanced the convergence of LAK cells to the lungs, liver, lymph nodes and bones. Therefore, splenectomy may augment the therapeutic effect of the adoptive transfer of LAK cells in pulmonary, hepatic, lymph node and bony metastases.

Original languageEnglish
Pages (from-to)223-227
Number of pages5
JournalProceedings of the National Science Council, Republic of China. Part B, Life sciences
Volume14
Issue number4
Publication statusPublished - Dec 1990
Externally publishedYes

Fingerprint

Lymphokine-Activated Killer Cells
Splenectomy
Lung
Lymph Nodes
Injections
Liver
Spleen
Bone and Bones
Adoptive Transfer
Therapeutic Uses
Tail
Veins
Neoplasm Metastasis
Kidney

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{c1557de2983c4ec6bdbfc7f1e651a9b8,
title = "The in vivo distribution of murine lymphokine activated killer cells in splenectomized host.",
abstract = "The in vivo distribution of intravenously injected lymphokine activated killer (LAK) cells, generated in vitro with rIL-2 from normal murine splenocytes, was studied in BALB/c mice and compared with that of normal splenocytes. Both normal splenocytes and LAK cells were labeled with 51Cr, and the results were analyzed at 6, 24, and 48 hours after injection by localization index as the parameter. After injection through tail veins of mice, LAK cells were found to migrate to the spleen, lungs, liver, lymph nodes, bones and the kidneys. The apparent increased distribution pattern of LAK cells to the lung at 6 and 24 hours after injection was not detected when normal splenocytes were injected. Since almost one third of the injected LAK cells were found to localize in the spleen, it was postulated that splenectomy would affect the in vivo organ distribution of LAK cells. Accordingly, the in vivo distribution of LAK cells in splenectomized mice was further investigated. Results indicated that splenectomy enhanced the convergence of LAK cells to the lungs, liver, lymph nodes and bones. Therefore, splenectomy may augment the therapeutic effect of the adoptive transfer of LAK cells in pulmonary, hepatic, lymph node and bony metastases.",
author = "Chao, {T. Y.} and Chu, {T. M.}",
year = "1990",
month = "12",
language = "English",
volume = "14",
pages = "223--227",
journal = "Proceedings of the National Science Council, Republic of China. Part B, Life sciences",
issn = "0255-6596",
publisher = "National Science Council",
number = "4",

}

TY - JOUR

T1 - The in vivo distribution of murine lymphokine activated killer cells in splenectomized host.

AU - Chao, T. Y.

AU - Chu, T. M.

PY - 1990/12

Y1 - 1990/12

N2 - The in vivo distribution of intravenously injected lymphokine activated killer (LAK) cells, generated in vitro with rIL-2 from normal murine splenocytes, was studied in BALB/c mice and compared with that of normal splenocytes. Both normal splenocytes and LAK cells were labeled with 51Cr, and the results were analyzed at 6, 24, and 48 hours after injection by localization index as the parameter. After injection through tail veins of mice, LAK cells were found to migrate to the spleen, lungs, liver, lymph nodes, bones and the kidneys. The apparent increased distribution pattern of LAK cells to the lung at 6 and 24 hours after injection was not detected when normal splenocytes were injected. Since almost one third of the injected LAK cells were found to localize in the spleen, it was postulated that splenectomy would affect the in vivo organ distribution of LAK cells. Accordingly, the in vivo distribution of LAK cells in splenectomized mice was further investigated. Results indicated that splenectomy enhanced the convergence of LAK cells to the lungs, liver, lymph nodes and bones. Therefore, splenectomy may augment the therapeutic effect of the adoptive transfer of LAK cells in pulmonary, hepatic, lymph node and bony metastases.

AB - The in vivo distribution of intravenously injected lymphokine activated killer (LAK) cells, generated in vitro with rIL-2 from normal murine splenocytes, was studied in BALB/c mice and compared with that of normal splenocytes. Both normal splenocytes and LAK cells were labeled with 51Cr, and the results were analyzed at 6, 24, and 48 hours after injection by localization index as the parameter. After injection through tail veins of mice, LAK cells were found to migrate to the spleen, lungs, liver, lymph nodes, bones and the kidneys. The apparent increased distribution pattern of LAK cells to the lung at 6 and 24 hours after injection was not detected when normal splenocytes were injected. Since almost one third of the injected LAK cells were found to localize in the spleen, it was postulated that splenectomy would affect the in vivo organ distribution of LAK cells. Accordingly, the in vivo distribution of LAK cells in splenectomized mice was further investigated. Results indicated that splenectomy enhanced the convergence of LAK cells to the lungs, liver, lymph nodes and bones. Therefore, splenectomy may augment the therapeutic effect of the adoptive transfer of LAK cells in pulmonary, hepatic, lymph node and bony metastases.

UR - http://www.scopus.com/inward/record.url?scp=0025619801&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025619801&partnerID=8YFLogxK

M3 - Article

VL - 14

SP - 223

EP - 227

JO - Proceedings of the National Science Council, Republic of China. Part B, Life sciences

JF - Proceedings of the National Science Council, Republic of China. Part B, Life sciences

SN - 0255-6596

IS - 4

ER -