23 Citations (Scopus)

Abstract

Objective: To investigate the in vitro effects of dehydroepiandrosterone (DHEA) on neonatal rat chondrocytes. Design: Chondrocytes isolated from neonatal rat cartilage were cultured in three-dimensionally agarose beads and were treated with DHEA. Methods: Primary culture of chondrocytes was harvested from newborn Wistar rats. The DHEA effects on chondrocyte activities were evaluated by analyzing chondrocyte proliferation, matrix protein synthesis, gene expressions of collagen, matrix metalloproteinase-1, -3 and -13 (MMP-1, -3 and -13), and cyclooxygenase-2 (COX-II), and protein synthesis of interleukin-6 (IL-6), prostaglandin E2 (PGE2) and tissue inhibitor of metalloproteinase-1 (TIMP-1). Results: The DHEA treatment did affect chondrocyte proliferation and glycosaminoglycan (GAG) synthesis. DHEA suppressed the expression of MMP-1, -3 and -13 genes and PGE2 protein synthesis enhanced by lipopolysaccharide (LPS) while the COX-II and inducible nitric oxide synthase (iNOS) gene expressions were down-regulated by DHEA. Conclusions: Our study demonstrates that DHEA has an ability to modulate the imbalance between MMPs and PGE2 in the neonatal chondrocytes which suggest that it has a potential protective role against articular cartilage damage.

Original languageEnglish
Pages (from-to)238-249
Number of pages12
JournalOsteoarthritis and Cartilage
Volume14
Issue number3
DOIs
Publication statusPublished - Mar 2006

Fingerprint

Dehydroepiandrosterone
Chondrocytes
Metabolism
Rats
Cartilage
Proteins
Gene expression
Matrix Metalloproteinases
Dinoprostone
Nitric oxide
Collagen
Genes
Gene Expression
Matrix Metalloproteinase 3
Matrix Metalloproteinase 1
Tissue
Tissue Inhibitor of Metalloproteinase-1
Articular Cartilage
Nitric Oxide Synthase Type II
Cyclooxygenase 2

Keywords

  • Arthritis
  • Chondrocyte
  • Dehydroepiandrosterone
  • Metalloproteinase
  • Tissue inhibitor of metalloproteinase-1

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

The in vitro effects of dehydroepiandrosterone on chondrocyte metabolism. / Sun, J. S.; Wu, C. X.; Tsuang, Y. H.; Chen, L. T.; Sheu, Shiow Yunn.

In: Osteoarthritis and Cartilage, Vol. 14, No. 3, 03.2006, p. 238-249.

Research output: Contribution to journalArticle

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abstract = "Objective: To investigate the in vitro effects of dehydroepiandrosterone (DHEA) on neonatal rat chondrocytes. Design: Chondrocytes isolated from neonatal rat cartilage were cultured in three-dimensionally agarose beads and were treated with DHEA. Methods: Primary culture of chondrocytes was harvested from newborn Wistar rats. The DHEA effects on chondrocyte activities were evaluated by analyzing chondrocyte proliferation, matrix protein synthesis, gene expressions of collagen, matrix metalloproteinase-1, -3 and -13 (MMP-1, -3 and -13), and cyclooxygenase-2 (COX-II), and protein synthesis of interleukin-6 (IL-6), prostaglandin E2 (PGE2) and tissue inhibitor of metalloproteinase-1 (TIMP-1). Results: The DHEA treatment did affect chondrocyte proliferation and glycosaminoglycan (GAG) synthesis. DHEA suppressed the expression of MMP-1, -3 and -13 genes and PGE2 protein synthesis enhanced by lipopolysaccharide (LPS) while the COX-II and inducible nitric oxide synthase (iNOS) gene expressions were down-regulated by DHEA. Conclusions: Our study demonstrates that DHEA has an ability to modulate the imbalance between MMPs and PGE2 in the neonatal chondrocytes which suggest that it has a potential protective role against articular cartilage damage.",
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AU - Sheu, Shiow Yunn

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N2 - Objective: To investigate the in vitro effects of dehydroepiandrosterone (DHEA) on neonatal rat chondrocytes. Design: Chondrocytes isolated from neonatal rat cartilage were cultured in three-dimensionally agarose beads and were treated with DHEA. Methods: Primary culture of chondrocytes was harvested from newborn Wistar rats. The DHEA effects on chondrocyte activities were evaluated by analyzing chondrocyte proliferation, matrix protein synthesis, gene expressions of collagen, matrix metalloproteinase-1, -3 and -13 (MMP-1, -3 and -13), and cyclooxygenase-2 (COX-II), and protein synthesis of interleukin-6 (IL-6), prostaglandin E2 (PGE2) and tissue inhibitor of metalloproteinase-1 (TIMP-1). Results: The DHEA treatment did affect chondrocyte proliferation and glycosaminoglycan (GAG) synthesis. DHEA suppressed the expression of MMP-1, -3 and -13 genes and PGE2 protein synthesis enhanced by lipopolysaccharide (LPS) while the COX-II and inducible nitric oxide synthase (iNOS) gene expressions were down-regulated by DHEA. Conclusions: Our study demonstrates that DHEA has an ability to modulate the imbalance between MMPs and PGE2 in the neonatal chondrocytes which suggest that it has a potential protective role against articular cartilage damage.

AB - Objective: To investigate the in vitro effects of dehydroepiandrosterone (DHEA) on neonatal rat chondrocytes. Design: Chondrocytes isolated from neonatal rat cartilage were cultured in three-dimensionally agarose beads and were treated with DHEA. Methods: Primary culture of chondrocytes was harvested from newborn Wistar rats. The DHEA effects on chondrocyte activities were evaluated by analyzing chondrocyte proliferation, matrix protein synthesis, gene expressions of collagen, matrix metalloproteinase-1, -3 and -13 (MMP-1, -3 and -13), and cyclooxygenase-2 (COX-II), and protein synthesis of interleukin-6 (IL-6), prostaglandin E2 (PGE2) and tissue inhibitor of metalloproteinase-1 (TIMP-1). Results: The DHEA treatment did affect chondrocyte proliferation and glycosaminoglycan (GAG) synthesis. DHEA suppressed the expression of MMP-1, -3 and -13 genes and PGE2 protein synthesis enhanced by lipopolysaccharide (LPS) while the COX-II and inducible nitric oxide synthase (iNOS) gene expressions were down-regulated by DHEA. Conclusions: Our study demonstrates that DHEA has an ability to modulate the imbalance between MMPs and PGE2 in the neonatal chondrocytes which suggest that it has a potential protective role against articular cartilage damage.

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