The in vitro antioxidant activity of trilinolein and other lipid-related natural substances as measured by enhanced chemiluminescence

Paul Chan, Juei Tang Cheng, Chiung Wen Tsao, Chiang Shan Niu, Chuang Ye Hong

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

There is abundant evidence for the premise that oxygen-derived free radicals (OFR) mediate ischemia/reperfusion injury to the myocardium. OFR scavengers such as superoxide dismutase can effectively reduce damage through lipid peroxidation during ischemia/reperfusion. Enhanced chemiluminescence, which has been used to measure OFR, was used to measure the antioxidant activity of fatty acids (palmitic and linoleic acid) and triglycerides (triolein, tristearin) and natural plant antioxidants (magnolol, catechin, trilinolein). Trilinolein, which has recently been isolated from natural products, as well as the well-known water soluble analogue of vitamine E - Trolox, were used as control. During pretreatment with chemicals, at concentrations of 10-9 to 10-7 M, enhanced chemiluminescence of linoleic acid (C 18:2) showed a dose-responsive reduction of OFR with a maximal mean reduction of -31.9% when compared to baseline. A saturated fatty acid such as palmitic acid (C 16:0) showed only relatively weak antioxidant activity at concentrations of 10-7 to 10-6 M with a maximum reduction of OFR of - 15.2% only. Control chemicals such as trilinolein and Trolox showed significant antioxidant activity. At concentrations between 10-10 and 10-6 M and trilinolein has the most potent antioxidant activity with a maximal mean reduction of OFR of - 48.0%, whereas Trolox showed only -39.2%. As for the natural plant antioxidants, only catechin showed potent antioxidant activity (-40%). Polyunsaturated triglycerides such as triolein (oleic acid, C 18:1) also possess significant OFR scavenging effect (-31.9%) whilst saturated triglycerides such as tristearin (stearic acid, C 18:0) had only relatively weak antioxidant activity (-15.2%). Generally, the antioxidant activity of unsaturated compounds is stronger than saturated compounds; double-bond existence may partially explain this phenomenon.

Original languageEnglish
Pages (from-to)2067-2073
Number of pages7
JournalLife Sciences
Volume59
Issue number24
DOIs
Publication statusPublished - Nov 8 1996
Externally publishedYes

Fingerprint

Chemiluminescence
Luminescence
Antioxidants
Lipids
Free Radicals
Oxygen
Triolein
Triglycerides
Palmitic Acid
Catechin
Linoleic Acid
Fatty Acids
Unsaturated compounds
trilinolein
In Vitro Techniques
Free Radical Scavengers
Scavenging
Oleic Acid
Reperfusion Injury
Biological Products

Keywords

  • antioxidant activity
  • enhanced chemiluminescence
  • oxygen-derived free radicals
  • trilinolein

ASJC Scopus subject areas

  • Pharmacology

Cite this

The in vitro antioxidant activity of trilinolein and other lipid-related natural substances as measured by enhanced chemiluminescence. / Chan, Paul; Cheng, Juei Tang; Tsao, Chiung Wen; Niu, Chiang Shan; Hong, Chuang Ye.

In: Life Sciences, Vol. 59, No. 24, 08.11.1996, p. 2067-2073.

Research output: Contribution to journalArticle

Chan, Paul ; Cheng, Juei Tang ; Tsao, Chiung Wen ; Niu, Chiang Shan ; Hong, Chuang Ye. / The in vitro antioxidant activity of trilinolein and other lipid-related natural substances as measured by enhanced chemiluminescence. In: Life Sciences. 1996 ; Vol. 59, No. 24. pp. 2067-2073.
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abstract = "There is abundant evidence for the premise that oxygen-derived free radicals (OFR) mediate ischemia/reperfusion injury to the myocardium. OFR scavengers such as superoxide dismutase can effectively reduce damage through lipid peroxidation during ischemia/reperfusion. Enhanced chemiluminescence, which has been used to measure OFR, was used to measure the antioxidant activity of fatty acids (palmitic and linoleic acid) and triglycerides (triolein, tristearin) and natural plant antioxidants (magnolol, catechin, trilinolein). Trilinolein, which has recently been isolated from natural products, as well as the well-known water soluble analogue of vitamine E - Trolox, were used as control. During pretreatment with chemicals, at concentrations of 10-9 to 10-7 M, enhanced chemiluminescence of linoleic acid (C 18:2) showed a dose-responsive reduction of OFR with a maximal mean reduction of -31.9{\%} when compared to baseline. A saturated fatty acid such as palmitic acid (C 16:0) showed only relatively weak antioxidant activity at concentrations of 10-7 to 10-6 M with a maximum reduction of OFR of - 15.2{\%} only. Control chemicals such as trilinolein and Trolox showed significant antioxidant activity. At concentrations between 10-10 and 10-6 M and trilinolein has the most potent antioxidant activity with a maximal mean reduction of OFR of - 48.0{\%}, whereas Trolox showed only -39.2{\%}. As for the natural plant antioxidants, only catechin showed potent antioxidant activity (-40{\%}). Polyunsaturated triglycerides such as triolein (oleic acid, C 18:1) also possess significant OFR scavenging effect (-31.9{\%}) whilst saturated triglycerides such as tristearin (stearic acid, C 18:0) had only relatively weak antioxidant activity (-15.2{\%}). Generally, the antioxidant activity of unsaturated compounds is stronger than saturated compounds; double-bond existence may partially explain this phenomenon.",
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AB - There is abundant evidence for the premise that oxygen-derived free radicals (OFR) mediate ischemia/reperfusion injury to the myocardium. OFR scavengers such as superoxide dismutase can effectively reduce damage through lipid peroxidation during ischemia/reperfusion. Enhanced chemiluminescence, which has been used to measure OFR, was used to measure the antioxidant activity of fatty acids (palmitic and linoleic acid) and triglycerides (triolein, tristearin) and natural plant antioxidants (magnolol, catechin, trilinolein). Trilinolein, which has recently been isolated from natural products, as well as the well-known water soluble analogue of vitamine E - Trolox, were used as control. During pretreatment with chemicals, at concentrations of 10-9 to 10-7 M, enhanced chemiluminescence of linoleic acid (C 18:2) showed a dose-responsive reduction of OFR with a maximal mean reduction of -31.9% when compared to baseline. A saturated fatty acid such as palmitic acid (C 16:0) showed only relatively weak antioxidant activity at concentrations of 10-7 to 10-6 M with a maximum reduction of OFR of - 15.2% only. Control chemicals such as trilinolein and Trolox showed significant antioxidant activity. At concentrations between 10-10 and 10-6 M and trilinolein has the most potent antioxidant activity with a maximal mean reduction of OFR of - 48.0%, whereas Trolox showed only -39.2%. As for the natural plant antioxidants, only catechin showed potent antioxidant activity (-40%). Polyunsaturated triglycerides such as triolein (oleic acid, C 18:1) also possess significant OFR scavenging effect (-31.9%) whilst saturated triglycerides such as tristearin (stearic acid, C 18:0) had only relatively weak antioxidant activity (-15.2%). Generally, the antioxidant activity of unsaturated compounds is stronger than saturated compounds; double-bond existence may partially explain this phenomenon.

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