Abstract

The transcription factor GATA3 plays a significant role in mammary gland development and differentiation. We analyzed expression of GATA3 in breast cancer (BC) cell lines and clinical specimens from BC patients in Taiwan. Semiquantitative reverse-transcription polymerase chain reaction (RT-PCR), quantitative real-time PCR were carried out to determine the mRNA level of GATA3 from 241 pairs of matched tumor and adjacent normal tissues from anonymous female donors. GATA3 immunohistochemistry (IHC) staining and H-score were performed (n = 25). Inducing and silencing of GATA3 were done by exposure MCF-7 cell line to nicotine or curcumin, respectively. GATA3 expression was detected in most of the estrogen receptor–positive (ER+) tumor specimens (176/241, 73%) compared with paired normal tissues (65/241, 27%) (P <.001). The GATA3 level was highest in Luminal A, and independent t-tests revealed higher GATA3 was associated with ER+ (P =.018) and BC stages (stage II, and stage IV). Nuclear protein expression of GATA3 was detected in tumor tissues (P <.001) with higher H-score in Luminal A patients (P =.012). Kaplan–Meier survival analyses showed that ER+/progesterone receptor (PgR)+ and lower grade BC patients with relatively high GATA3 had better clinical overall survival (OS). GATA3 regulates ERα and BCL-2 as BC luminal subtype markers. Cox univariate and multivariate analyses demonstrated that the expression of GATA3 was an effective predictor of the risk of death. We demonstrated a correlation between GATA3 expression and only ER+ and suggest that a higher GATA3 expression is a good prognostic factor for OS for ER+ BC patients. © 2018 Elsevier Inc.
Original languageEnglish
Pages (from-to)219-230
Number of pages12
JournalHuman Pathology
Volume80
DOIs
Publication statusPublished - 2018

Keywords

  • Breast cancer
  • Estrogen receptor
  • GATA3
  • Overall survival
  • Prognostic factor
  • messenger RNA
  • nuclear protein
  • protein bcl 2
  • tamoxifen
  • transcription factor GATA 3
  • trastuzumab
  • adult
  • Article
  • breast cancer
  • cancer chemotherapy
  • cancer mortality
  • cancer patient
  • cancer prognosis
  • cancer radiotherapy
  • cancer survival
  • comparative study
  • estrogen receptor negative breast cancer
  • estrogen receptor positive breast cancer
  • gene silencing
  • human
  • human cell
  • human epidermal growth factor receptor 2 positive breast cancer
  • human tissue
  • immunohistochemistry
  • luminal A breast cancer
  • major clinical study
  • MCF-7 cell line
  • mRNA expression level
  • overall survival
  • progesterone receptor positive breast cancer
  • protein localization
  • quantitative analysis
  • real time polymerase chain reaction
  • reverse transcription polymerase chain reaction
  • Taiwan

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