The hypouricemic effect of Balanophora laxiflora extracts and derived phytochemicals in hyperuricemic mice

Shang Tse Ho, Yu Tang Tung, Chi Chang Huang, Chao Lin Kuo, Chi Chen Lin, Suh Ching Yang, Jyh Horng Wu

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8 Citations (Scopus)

Abstract

The objective of this study is to evaluate the lowering of uric acid using Balanophora laxiflora extracts and derived phytochemicals on potassium-oxonate-(PO-) induced hyperuricemia in mice. The results revealed that ethyl acetate (EtOAc) fraction of B. laxiflora extracts exhibited strong xanthine-oxidase-(XOD-) inhibitory activity. In addition, among the 10 subfractions (EA110) derived from EtOAc fraction, subfraction 8 (EA8) exhibited the best XOD-inhibitory activity. Four specific phytochemicals, 1-O-(E)-caffeoyl - D-glucopyranose (1), 1-O-(E)-p-coumaroyl - D-glucopyranose (2), 1,3-di-O-galloyl-4,6-(S)-hexahydroxydiphenoylβ- D-glucopyranose (3), and 1-O-(E)-caffeoyl-4,6-(S)-hexahydroxydiphenoyl - D-glucopyranose (4), were further isolated and identified from this subfraction. Compounds 3 and 4 exhibited the strongest XOD-inhibitory activity compared with other compounds, and both hydrolyzable tannins were determined to be noncompetitive inhibitors according to the Lineweaver-Burk plot. On the other hand, the in vivo hypouricemic effect in hyperuricemic mice was consistent with XOD-inhibitory activity, indicating that B. laxiflora extracts and derived phytochemicals could be potential candidates as new hypouricemic agents.

Original languageEnglish
Article number910152
JournalEvidence-based Complementary and Alternative Medicine
Volume2012
DOIs
Publication statusPublished - 2012

ASJC Scopus subject areas

  • Complementary and alternative medicine

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