The human papillomavirus-16 (HPV-16) oncoprotein E7 conjugates with and mediates the role of the transforming growth factor-beta inducible early gene 1 (TIEG1) in apoptosis

Hung Shu Chang, Ching Hui Lin, Chien Hui Yang, Yuh Jin Liang, Winston C Y Yu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The human papillomavirus (HPV) oncoprotein E7 is a major transforming protein. The E7 protein does not possess intrinsic enzymatic activity, but rather functions through direct and indirect interactions with cellular proteins, several of which are well known cellular tumor suppressors. Using the yeast two-hybrid system, we found that transforming growth factor-beta inducible early gene 1 (TIEG1), a member of the Krüppel-like family (KLF) that has been implicated as a putative tumor suppressor, interacts and forms a specific complex with HPV-16 E7. TIEG1 has been shown to mimic the effects of TGF-beta in various carcinoma cells and plays a critical role in the apoptotic cascade. Our results indicate that E7 binds to the C-terminus of TIEG1 and induces its degradation via the ubiquitin pathway. E7 not only increased the ubiquitination of TIEG1 but also influenced the ability of TIEG1 to affect apoptosis. Our results suggest that suppression of TIEG1-mediated signaling by E7 may contribute to HPV-associated carcinogenesis.

Original languageEnglish
Pages (from-to)1831-1839
Number of pages9
JournalInternational Journal of Biochemistry and Cell Biology
Volume42
Issue number11
DOIs
Publication statusPublished - Nov 2010
Externally publishedYes

Fingerprint

Human papillomavirus 16
Oncogene Proteins
Transforming Growth Factor beta
Genes
Apoptosis
Tumors
Two-Hybrid System Techniques
Proteins
Ubiquitination
Ubiquitin
Hybrid systems
Yeast
Neoplasms
Carcinogenesis
Cells
Carcinoma
Degradation

Keywords

  • Apoptosis
  • Cervical cancer
  • E7
  • HPV
  • TIEG1

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

The human papillomavirus-16 (HPV-16) oncoprotein E7 conjugates with and mediates the role of the transforming growth factor-beta inducible early gene 1 (TIEG1) in apoptosis. / Chang, Hung Shu; Lin, Ching Hui; Yang, Chien Hui; Liang, Yuh Jin; Yu, Winston C Y.

In: International Journal of Biochemistry and Cell Biology, Vol. 42, No. 11, 11.2010, p. 1831-1839.

Research output: Contribution to journalArticle

@article{879d162ff1014a108ab72019821eda1e,
title = "The human papillomavirus-16 (HPV-16) oncoprotein E7 conjugates with and mediates the role of the transforming growth factor-beta inducible early gene 1 (TIEG1) in apoptosis",
abstract = "The human papillomavirus (HPV) oncoprotein E7 is a major transforming protein. The E7 protein does not possess intrinsic enzymatic activity, but rather functions through direct and indirect interactions with cellular proteins, several of which are well known cellular tumor suppressors. Using the yeast two-hybrid system, we found that transforming growth factor-beta inducible early gene 1 (TIEG1), a member of the Kr{\"u}ppel-like family (KLF) that has been implicated as a putative tumor suppressor, interacts and forms a specific complex with HPV-16 E7. TIEG1 has been shown to mimic the effects of TGF-beta in various carcinoma cells and plays a critical role in the apoptotic cascade. Our results indicate that E7 binds to the C-terminus of TIEG1 and induces its degradation via the ubiquitin pathway. E7 not only increased the ubiquitination of TIEG1 but also influenced the ability of TIEG1 to affect apoptosis. Our results suggest that suppression of TIEG1-mediated signaling by E7 may contribute to HPV-associated carcinogenesis.",
keywords = "Apoptosis, Cervical cancer, E7, HPV, TIEG1",
author = "Chang, {Hung Shu} and Lin, {Ching Hui} and Yang, {Chien Hui} and Liang, {Yuh Jin} and Yu, {Winston C Y}",
year = "2010",
month = "11",
doi = "10.1016/j.biocel.2010.07.019",
language = "English",
volume = "42",
pages = "1831--1839",
journal = "International Journal of Biochemistry and Cell Biology",
issn = "1357-2725",
publisher = "Elsevier Limited",
number = "11",

}

TY - JOUR

T1 - The human papillomavirus-16 (HPV-16) oncoprotein E7 conjugates with and mediates the role of the transforming growth factor-beta inducible early gene 1 (TIEG1) in apoptosis

AU - Chang, Hung Shu

AU - Lin, Ching Hui

AU - Yang, Chien Hui

AU - Liang, Yuh Jin

AU - Yu, Winston C Y

PY - 2010/11

Y1 - 2010/11

N2 - The human papillomavirus (HPV) oncoprotein E7 is a major transforming protein. The E7 protein does not possess intrinsic enzymatic activity, but rather functions through direct and indirect interactions with cellular proteins, several of which are well known cellular tumor suppressors. Using the yeast two-hybrid system, we found that transforming growth factor-beta inducible early gene 1 (TIEG1), a member of the Krüppel-like family (KLF) that has been implicated as a putative tumor suppressor, interacts and forms a specific complex with HPV-16 E7. TIEG1 has been shown to mimic the effects of TGF-beta in various carcinoma cells and plays a critical role in the apoptotic cascade. Our results indicate that E7 binds to the C-terminus of TIEG1 and induces its degradation via the ubiquitin pathway. E7 not only increased the ubiquitination of TIEG1 but also influenced the ability of TIEG1 to affect apoptosis. Our results suggest that suppression of TIEG1-mediated signaling by E7 may contribute to HPV-associated carcinogenesis.

AB - The human papillomavirus (HPV) oncoprotein E7 is a major transforming protein. The E7 protein does not possess intrinsic enzymatic activity, but rather functions through direct and indirect interactions with cellular proteins, several of which are well known cellular tumor suppressors. Using the yeast two-hybrid system, we found that transforming growth factor-beta inducible early gene 1 (TIEG1), a member of the Krüppel-like family (KLF) that has been implicated as a putative tumor suppressor, interacts and forms a specific complex with HPV-16 E7. TIEG1 has been shown to mimic the effects of TGF-beta in various carcinoma cells and plays a critical role in the apoptotic cascade. Our results indicate that E7 binds to the C-terminus of TIEG1 and induces its degradation via the ubiquitin pathway. E7 not only increased the ubiquitination of TIEG1 but also influenced the ability of TIEG1 to affect apoptosis. Our results suggest that suppression of TIEG1-mediated signaling by E7 may contribute to HPV-associated carcinogenesis.

KW - Apoptosis

KW - Cervical cancer

KW - E7

KW - HPV

KW - TIEG1

UR - http://www.scopus.com/inward/record.url?scp=77957279274&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77957279274&partnerID=8YFLogxK

U2 - 10.1016/j.biocel.2010.07.019

DO - 10.1016/j.biocel.2010.07.019

M3 - Article

VL - 42

SP - 1831

EP - 1839

JO - International Journal of Biochemistry and Cell Biology

JF - International Journal of Biochemistry and Cell Biology

SN - 1357-2725

IS - 11

ER -