The human papillomavirus-16 (HPV-16) oncoprotein E7 conjugates with and mediates the role of the transforming growth factor-beta inducible early gene 1 (TIEG1) in apoptosis

Hung Shu Chang, Ching Hui Lin, Chien Hui Yang, Yuh Jin Liang, Winston C Y Yu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The human papillomavirus (HPV) oncoprotein E7 is a major transforming protein. The E7 protein does not possess intrinsic enzymatic activity, but rather functions through direct and indirect interactions with cellular proteins, several of which are well known cellular tumor suppressors. Using the yeast two-hybrid system, we found that transforming growth factor-beta inducible early gene 1 (TIEG1), a member of the Krüppel-like family (KLF) that has been implicated as a putative tumor suppressor, interacts and forms a specific complex with HPV-16 E7. TIEG1 has been shown to mimic the effects of TGF-beta in various carcinoma cells and plays a critical role in the apoptotic cascade. Our results indicate that E7 binds to the C-terminus of TIEG1 and induces its degradation via the ubiquitin pathway. E7 not only increased the ubiquitination of TIEG1 but also influenced the ability of TIEG1 to affect apoptosis. Our results suggest that suppression of TIEG1-mediated signaling by E7 may contribute to HPV-associated carcinogenesis.

Original languageEnglish
Pages (from-to)1831-1839
Number of pages9
JournalInternational Journal of Biochemistry and Cell Biology
Volume42
Issue number11
DOIs
Publication statusPublished - Nov 2010
Externally publishedYes

Keywords

  • Apoptosis
  • Cervical cancer
  • E7
  • HPV
  • TIEG1

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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