The human liver glutathione s-transferase gene superfamily: Expression and chromosome mapping of an Hb subunit cDNA

Jeff L. Dejong, Chi Ming Chang, Jacqueline Whang-Peng, Turid Knutsen, Chen Pei D. Tu

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

We have isolated from a λgtl0 cDNA library a clone λGTH4 which encodes a human liver glutathione S-transferase Hb subunit, designated as subunit 4. Expression of this cDNA in E. coli and subsequent purification and immunoblotting analysis provided a definitive assignment of a structure and function relationship. RNA blot hybridization with human liver poly(A) RNA revealed a single band of ∼1200 nucleotides, comparable in size to the rat brain Yb3 mRNA. Divergence analysis of amino acid replacement sites in subunit 4 relative to the four rat Yb subunits revealed that it is most closely related to the brain-specific Yb3 subunit. This conclusion is further substantiated by the nucleotide sequence homology between λGTH4 and the Yb3 cDNA in their 3′ untranslated region. In situ chromosome mapping has located this glutathione S-transferase gene in the region of p31 on chromosome 1. Results from many laboratories, including ours, indicate that the human glutathione S-transferases are encoded by a gene superfamily which is located on at least two different chromosomes.

Original languageEnglish
Pages (from-to)8541-8554
Number of pages14
JournalNucleic Acids Research
Volume16
Issue number17
DOIs
Publication statusPublished - Sep 12 1988
Externally publishedYes

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Chromosome Mapping
Glutathione Transferase
Complementary DNA
Gene Expression
Liver
Messenger RNA
Chromosomes, Human, Pair 1
Brain
3' Untranslated Regions
Sequence Homology
Gene Library
Immunoblotting
Genes
Nucleotides
Clone Cells
Chromosomes
RNA
Escherichia coli
Amino Acids

ASJC Scopus subject areas

  • Genetics

Cite this

The human liver glutathione s-transferase gene superfamily : Expression and chromosome mapping of an Hb subunit cDNA. / Dejong, Jeff L.; Chang, Chi Ming; Whang-Peng, Jacqueline; Knutsen, Turid; Tu, Chen Pei D.

In: Nucleic Acids Research, Vol. 16, No. 17, 12.09.1988, p. 8541-8554.

Research output: Contribution to journalArticle

Dejong, Jeff L. ; Chang, Chi Ming ; Whang-Peng, Jacqueline ; Knutsen, Turid ; Tu, Chen Pei D. / The human liver glutathione s-transferase gene superfamily : Expression and chromosome mapping of an Hb subunit cDNA. In: Nucleic Acids Research. 1988 ; Vol. 16, No. 17. pp. 8541-8554.
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