The human FGF-8 gene localizes on chromosome 10q24 and is subjected to induction by androgen in breast cancer cells

Robert A. Payson, Jackson Wu, Yang Liu, Ing Ming Chiu

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Androgen-induced growth factor (AIGF or FGF-8) was originally isolated from the conditioned medium of an androgen-dependent Shionogi carcinoma, SC-3, cell line. It shares structural similarity with other members of the FGF family. The temporal and spatial expression patterns of the FGF-8 gene suggest its involvement in gastrulation, regionalization of the brain, and organogenesis of the limb and face as an embryonic epithelial factor. In the adult, expression of FGF-8 is restricted to gonads including testes and ovaries. Since FGF-8 is identified as a corroborating gene in MMTV-induced mammary tumors in Wnt-1 transgenic mice and because FGF-8 manifested its autocrine mitogenic activity in SC-3 cells, it is possible that aberrant expression of FGF-8 may be present in human cancers which are hormone dependent. However, very little is known about human FGF-8. To determine whether FGF-8 plays a role in human breast cancer, we have isolated the full-length cDNA from SK-BR-3 breast cancer cells. We have also isolated the corresponding genomic DNA in a P1 cloning vector. The FGF-8 gene has been mapped to chromosome 10q24 using both somatic cell hybrid genetic analysis and fluorescence in situ hybridization. Finally, we show that FGF-8 gene expression in a human breast cancer cell line, MDA-MB-231, is inducible by androgen. The findings presented here will facilitate our understanding of the molecular mechanism underlying hormone-responsive breast and prostate cancers.

Original languageEnglish
Pages (from-to)47-53
Number of pages7
JournalOncogene
Volume13
Issue number1
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Androgens
Chromosomes
Breast Neoplasms
Genes
Fibroblast Growth Factor 8
Hormones
Cell Line
Genetic Vectors
Gastrulation
Organogenesis
Hybrid Cells
Gonads
Conditioned Culture Medium
Fluorescence In Situ Hybridization
Transgenic Mice
Testis
Ovary
Prostatic Neoplasms
Extremities
Complementary DNA

Keywords

  • Breast cancer
  • Cloning
  • Fibroblast growth factor
  • Fluorescence in situ hybridization
  • Steroid hormone

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

The human FGF-8 gene localizes on chromosome 10q24 and is subjected to induction by androgen in breast cancer cells. / Payson, Robert A.; Wu, Jackson; Liu, Yang; Chiu, Ing Ming.

In: Oncogene, Vol. 13, No. 1, 1996, p. 47-53.

Research output: Contribution to journalArticle

@article{8ad97c8174134d8489510e365d1efde6,
title = "The human FGF-8 gene localizes on chromosome 10q24 and is subjected to induction by androgen in breast cancer cells",
abstract = "Androgen-induced growth factor (AIGF or FGF-8) was originally isolated from the conditioned medium of an androgen-dependent Shionogi carcinoma, SC-3, cell line. It shares structural similarity with other members of the FGF family. The temporal and spatial expression patterns of the FGF-8 gene suggest its involvement in gastrulation, regionalization of the brain, and organogenesis of the limb and face as an embryonic epithelial factor. In the adult, expression of FGF-8 is restricted to gonads including testes and ovaries. Since FGF-8 is identified as a corroborating gene in MMTV-induced mammary tumors in Wnt-1 transgenic mice and because FGF-8 manifested its autocrine mitogenic activity in SC-3 cells, it is possible that aberrant expression of FGF-8 may be present in human cancers which are hormone dependent. However, very little is known about human FGF-8. To determine whether FGF-8 plays a role in human breast cancer, we have isolated the full-length cDNA from SK-BR-3 breast cancer cells. We have also isolated the corresponding genomic DNA in a P1 cloning vector. The FGF-8 gene has been mapped to chromosome 10q24 using both somatic cell hybrid genetic analysis and fluorescence in situ hybridization. Finally, we show that FGF-8 gene expression in a human breast cancer cell line, MDA-MB-231, is inducible by androgen. The findings presented here will facilitate our understanding of the molecular mechanism underlying hormone-responsive breast and prostate cancers.",
keywords = "Breast cancer, Cloning, Fibroblast growth factor, Fluorescence in situ hybridization, Steroid hormone",
author = "Payson, {Robert A.} and Jackson Wu and Yang Liu and Chiu, {Ing Ming}",
year = "1996",
language = "English",
volume = "13",
pages = "47--53",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - The human FGF-8 gene localizes on chromosome 10q24 and is subjected to induction by androgen in breast cancer cells

AU - Payson, Robert A.

AU - Wu, Jackson

AU - Liu, Yang

AU - Chiu, Ing Ming

PY - 1996

Y1 - 1996

N2 - Androgen-induced growth factor (AIGF or FGF-8) was originally isolated from the conditioned medium of an androgen-dependent Shionogi carcinoma, SC-3, cell line. It shares structural similarity with other members of the FGF family. The temporal and spatial expression patterns of the FGF-8 gene suggest its involvement in gastrulation, regionalization of the brain, and organogenesis of the limb and face as an embryonic epithelial factor. In the adult, expression of FGF-8 is restricted to gonads including testes and ovaries. Since FGF-8 is identified as a corroborating gene in MMTV-induced mammary tumors in Wnt-1 transgenic mice and because FGF-8 manifested its autocrine mitogenic activity in SC-3 cells, it is possible that aberrant expression of FGF-8 may be present in human cancers which are hormone dependent. However, very little is known about human FGF-8. To determine whether FGF-8 plays a role in human breast cancer, we have isolated the full-length cDNA from SK-BR-3 breast cancer cells. We have also isolated the corresponding genomic DNA in a P1 cloning vector. The FGF-8 gene has been mapped to chromosome 10q24 using both somatic cell hybrid genetic analysis and fluorescence in situ hybridization. Finally, we show that FGF-8 gene expression in a human breast cancer cell line, MDA-MB-231, is inducible by androgen. The findings presented here will facilitate our understanding of the molecular mechanism underlying hormone-responsive breast and prostate cancers.

AB - Androgen-induced growth factor (AIGF or FGF-8) was originally isolated from the conditioned medium of an androgen-dependent Shionogi carcinoma, SC-3, cell line. It shares structural similarity with other members of the FGF family. The temporal and spatial expression patterns of the FGF-8 gene suggest its involvement in gastrulation, regionalization of the brain, and organogenesis of the limb and face as an embryonic epithelial factor. In the adult, expression of FGF-8 is restricted to gonads including testes and ovaries. Since FGF-8 is identified as a corroborating gene in MMTV-induced mammary tumors in Wnt-1 transgenic mice and because FGF-8 manifested its autocrine mitogenic activity in SC-3 cells, it is possible that aberrant expression of FGF-8 may be present in human cancers which are hormone dependent. However, very little is known about human FGF-8. To determine whether FGF-8 plays a role in human breast cancer, we have isolated the full-length cDNA from SK-BR-3 breast cancer cells. We have also isolated the corresponding genomic DNA in a P1 cloning vector. The FGF-8 gene has been mapped to chromosome 10q24 using both somatic cell hybrid genetic analysis and fluorescence in situ hybridization. Finally, we show that FGF-8 gene expression in a human breast cancer cell line, MDA-MB-231, is inducible by androgen. The findings presented here will facilitate our understanding of the molecular mechanism underlying hormone-responsive breast and prostate cancers.

KW - Breast cancer

KW - Cloning

KW - Fibroblast growth factor

KW - Fluorescence in situ hybridization

KW - Steroid hormone

UR - http://www.scopus.com/inward/record.url?scp=0029977046&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029977046&partnerID=8YFLogxK

M3 - Article

C2 - 8700553

AN - SCOPUS:0029977046

VL - 13

SP - 47

EP - 53

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 1

ER -