The high salt form of poly(dG-dC)·poly(dG-dC) is left-handed Z-DNA: Raman spectra of crystals and solutions

Thomas J. Thamann, Richard C. Lord, Andrew H.J. Wang, Alexander Rich

Research output: Contribution to journalArticlepeer-review

237 Citations (Scopus)

Abstract

The laser-Raman spectra of crystalline d(CpGpCpGpCpG) and of aqueous poly(dG-dC)·poly(dG-dC) in high salt (4M NaCl) and low salt (0.lM NaCl) solutions have been measured and compared. The spectra of the crystal and the high-salt solution show a striking congruence, which indicates clearly that the high-salt form of the aqueous polymer has the left-handed Z-DNA structure of the crystalline oligomer. These two spectra differ substantially from that of the low-salt form of the polymer, which has been found previously to have spectral characteristics of the B-form of DNA. The high salt spectrum shows a unique line due to guanine residues at 625 cm-1 which should be useful for qualitative and possibly quantitative assessment of the amount of Z-structure present in a sample of DNA.

Original languageEnglish
Pages (from-to)5443-5458
Number of pages16
JournalNucleic Acids Research
Volume9
Issue number20
DOIs
Publication statusPublished - Oct 24 1981
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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