The further characterization of the specifically binding peptide to hepatocellular carcinoma

Yonge Guo, Caixia Ma, Guochao Nie, Chunyan Li, Jinling Wu, Juanjuan Han, Fenghuei Lin, How Tseng, Wei Chen, Weijiang Liang, Yingchun Hou

Research output: Contribution to journalArticle

Abstract

Previously, we reported a novel 12-mer peptide binding to hepatocellular carcinoma (HCC) cells specifically and sensitively, and it was screened by using a phage displayed peptide library. To identify the potential of this peptide to be used to the early diagnosis and drug delivery of HCC clinic, the synthesized peptide probe was further characterized by using the assays of cell immunofluorescence and tissue chip. The results of cultured cell immunofluorescence assay show that the peptide probe binds to HCC cells with satisfactory specificity and sensitivity. The results of tissue chip show that the peptide probe can bind to the most common types of HCC tissues, especially to the HCC tissues differentiated at grade II. All further characterized results indicate that this peptide is of the great potential to be used as a HCC targeting candidate for the early molecular imaging diagnosis and targeting nanoparticle or drug delivery in HCC clinic practice.

Original languageEnglish
Article number1450070
JournalBiomedical Engineering - Applications, Basis and Communications
Volume26
Issue number6
DOIs
Publication statusPublished - Dec 25 2014

Fingerprint

Peptides
Hepatocellular Carcinoma
Tissue
Drug delivery
Assays
Fluorescent Antibody Technique
Cells
Molecular imaging
Peptide Library
Bacteriophages
Molecular Imaging
Pharmaceutical Preparations
Nanoparticles
Early Diagnosis
Cultured Cells
Sensitivity and Specificity

Keywords

  • Hepatocellular carcinoma
  • Specific peptide
  • Targeting diagnosis and therapy

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Bioengineering

Cite this

The further characterization of the specifically binding peptide to hepatocellular carcinoma. / Guo, Yonge; Ma, Caixia; Nie, Guochao; Li, Chunyan; Wu, Jinling; Han, Juanjuan; Lin, Fenghuei; Tseng, How; Chen, Wei; Liang, Weijiang; Hou, Yingchun.

In: Biomedical Engineering - Applications, Basis and Communications, Vol. 26, No. 6, 1450070, 25.12.2014.

Research output: Contribution to journalArticle

Guo, Yonge ; Ma, Caixia ; Nie, Guochao ; Li, Chunyan ; Wu, Jinling ; Han, Juanjuan ; Lin, Fenghuei ; Tseng, How ; Chen, Wei ; Liang, Weijiang ; Hou, Yingchun. / The further characterization of the specifically binding peptide to hepatocellular carcinoma. In: Biomedical Engineering - Applications, Basis and Communications. 2014 ; Vol. 26, No. 6.
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AU - Han, Juanjuan

AU - Lin, Fenghuei

AU - Tseng, How

AU - Chen, Wei

AU - Liang, Weijiang

AU - Hou, Yingchun

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