The expression of HPV-16 E5 protein in squamous neoplastic changes in the uterine cervix

J. L. Chang, Y. P. Tsao, D. W. Liu, S. J. Huang, W. H. Lee, S. L. Chen

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

To investigate the expression of human papillomavirus type 16 (HPV-16) E5 protein in squamous neoplastic changes in the uterine cervix, the specific E5 antibody was generated and used to identify the expression of E5 protein in 40 cases of HPV-16-positive tissues and 5 previously identified HPV-negative normal cervical tissues. The results revealed that E5 protein was primarily expressed in the lower third of the epithelium in low-grade squamous intraepithelial lesions (SILs) and throughout the whole epithelium in high-grade SILs. In invasive squamous carcinoma, 60% of HPV-16-infected cancers which contained the episomal viral genome had the E5 gene, and could express E5 protein which was located throughout the whole epithelium. Previously, we documented the expression of type I growth factor receptors [ERBB1/EGFR (epidermal growth factor receptor), ERBB2, ERBB3 and ERBB4] in the full range of cervical neoplasias by immunohistochemistry assay. Hence, in this study, we extensively analyzed the correlation between the expression of E5 protein and the expression of type I growth factor receptors. Among 40 HPV-16-infected cervical neoplasias, we found that the expression of E5 protein was significantly correlated with either the expreession of the ERBB1 or the ERBB4 receptor.

Original languageEnglish
Pages (from-to)206-213
Number of pages8
JournalJournal of Biomedical Science
Volume8
Issue number2
DOIs
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • Papillomavirus type 16 E5 protein
  • Squamous carcinoma
  • Uterine cervix

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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