The ethyl acetate fraction of corn silk exhibits dual antioxidant and anti-glycation activities and protects insulin-secreting cells from glucotoxicity

Chia Chuan Chang, Wei Yuan, Hsiao Yuh Roan, Jia Ling Chang, Hsiu Chen Huang, Yu Ching Lee, Huey Jen Tsay, Hui Kang Liu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: In this study, we aimed to develop a Stigmata Maydis (corn silk) fraction with dual bio-activities against oxidative stress and protein glycation to protect β-cells from diabetes-induced failure. Methods: Corn silk fractions were prepared by partition and chemically characterised by thin-layer chromatography. Free radical scavenging assay, glycation assay, and cell-based viability test (neutral red) were employed to decide the best fraction. Cell death analysis was executed by annexin V/ Propidium iodide staining. Cell proliferation was measured by WST-1. Finally, β-cell function was evaluated by β-cell marker gene expression (RT-PCR) and acute insulin secretion test. Results: Four corn silk fractions were prepared from an ethanolic crude extract of corn silk. In vitro assays indicate ethyl acetate fraction (YMS-EA) was the most potent fraction. YMS-EA also attenuated the hydrogen peroxide- or methylglyoxal-induced induction of reactive oxygen species, reduction of cell viability, and inhibition of cell proliferation. However, YMS-EA was unable to prevent hydrogen peroxide-induced apoptosis or advanced glycation end-products-induced toxicity. Under hyperglycemic conditions, YMS-EA effectively reduced ROS levels, improved mRNA expression of insulin, glucokinase, and PDX-1, and enhanced glucose-stimulated insulin secretion. The similarity of bioactivities among apigenin, luteolin, and YMS-EA indicated that dual activities of YMS-EA might be derived from those compounds. Conclusions: We concluded that YMS-EA fraction could be developed as a preventive food agent against the glucotoxicity to β-cells in Type 2 diabetes.

Original languageEnglish
Article number432
JournalBMC Complementary and Alternative Medicine
Volume16
Issue number1
DOIs
Publication statusPublished - Nov 3 2016

Fingerprint

Silk
Insulin-Secreting Cells
Zea mays
Antioxidants
Insulin
Hydrogen Peroxide
Cell Survival
Cell Proliferation
Pyruvaldehyde
Neutral Red
Apigenin
Luteolin
Glucokinase
Christianity
Advanced Glycosylation End Products
Propidium
Annexin A5
Thin Layer Chromatography
Heat-Shock Proteins
Complex Mixtures

Keywords

  • Advanced glycation end products
  • Glucotoxicity
  • Methylglyoxal
  • Reactive oxygen species
  • Stigmata Maydis (corn silk)
  • β-cell failure

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

The ethyl acetate fraction of corn silk exhibits dual antioxidant and anti-glycation activities and protects insulin-secreting cells from glucotoxicity. / Chang, Chia Chuan; Yuan, Wei; Roan, Hsiao Yuh; Chang, Jia Ling; Huang, Hsiu Chen; Lee, Yu Ching; Tsay, Huey Jen; Liu, Hui Kang.

In: BMC Complementary and Alternative Medicine, Vol. 16, No. 1, 432, 03.11.2016.

Research output: Contribution to journalArticle

Chang, Chia Chuan ; Yuan, Wei ; Roan, Hsiao Yuh ; Chang, Jia Ling ; Huang, Hsiu Chen ; Lee, Yu Ching ; Tsay, Huey Jen ; Liu, Hui Kang. / The ethyl acetate fraction of corn silk exhibits dual antioxidant and anti-glycation activities and protects insulin-secreting cells from glucotoxicity. In: BMC Complementary and Alternative Medicine. 2016 ; Vol. 16, No. 1.
@article{7c39d623224445bd87019b548b6c109b,
title = "The ethyl acetate fraction of corn silk exhibits dual antioxidant and anti-glycation activities and protects insulin-secreting cells from glucotoxicity",
abstract = "Background: In this study, we aimed to develop a Stigmata Maydis (corn silk) fraction with dual bio-activities against oxidative stress and protein glycation to protect β-cells from diabetes-induced failure. Methods: Corn silk fractions were prepared by partition and chemically characterised by thin-layer chromatography. Free radical scavenging assay, glycation assay, and cell-based viability test (neutral red) were employed to decide the best fraction. Cell death analysis was executed by annexin V/ Propidium iodide staining. Cell proliferation was measured by WST-1. Finally, β-cell function was evaluated by β-cell marker gene expression (RT-PCR) and acute insulin secretion test. Results: Four corn silk fractions were prepared from an ethanolic crude extract of corn silk. In vitro assays indicate ethyl acetate fraction (YMS-EA) was the most potent fraction. YMS-EA also attenuated the hydrogen peroxide- or methylglyoxal-induced induction of reactive oxygen species, reduction of cell viability, and inhibition of cell proliferation. However, YMS-EA was unable to prevent hydrogen peroxide-induced apoptosis or advanced glycation end-products-induced toxicity. Under hyperglycemic conditions, YMS-EA effectively reduced ROS levels, improved mRNA expression of insulin, glucokinase, and PDX-1, and enhanced glucose-stimulated insulin secretion. The similarity of bioactivities among apigenin, luteolin, and YMS-EA indicated that dual activities of YMS-EA might be derived from those compounds. Conclusions: We concluded that YMS-EA fraction could be developed as a preventive food agent against the glucotoxicity to β-cells in Type 2 diabetes.",
keywords = "Advanced glycation end products, Glucotoxicity, Methylglyoxal, Reactive oxygen species, Stigmata Maydis (corn silk), β-cell failure",
author = "Chang, {Chia Chuan} and Wei Yuan and Roan, {Hsiao Yuh} and Chang, {Jia Ling} and Huang, {Hsiu Chen} and Lee, {Yu Ching} and Tsay, {Huey Jen} and Liu, {Hui Kang}",
year = "2016",
month = "11",
day = "3",
doi = "10.1186/s12906-016-1382-8",
language = "English",
volume = "16",
journal = "BMC Complementary and Alternative Medicine",
issn = "1472-6882",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - The ethyl acetate fraction of corn silk exhibits dual antioxidant and anti-glycation activities and protects insulin-secreting cells from glucotoxicity

AU - Chang, Chia Chuan

AU - Yuan, Wei

AU - Roan, Hsiao Yuh

AU - Chang, Jia Ling

AU - Huang, Hsiu Chen

AU - Lee, Yu Ching

AU - Tsay, Huey Jen

AU - Liu, Hui Kang

PY - 2016/11/3

Y1 - 2016/11/3

N2 - Background: In this study, we aimed to develop a Stigmata Maydis (corn silk) fraction with dual bio-activities against oxidative stress and protein glycation to protect β-cells from diabetes-induced failure. Methods: Corn silk fractions were prepared by partition and chemically characterised by thin-layer chromatography. Free radical scavenging assay, glycation assay, and cell-based viability test (neutral red) were employed to decide the best fraction. Cell death analysis was executed by annexin V/ Propidium iodide staining. Cell proliferation was measured by WST-1. Finally, β-cell function was evaluated by β-cell marker gene expression (RT-PCR) and acute insulin secretion test. Results: Four corn silk fractions were prepared from an ethanolic crude extract of corn silk. In vitro assays indicate ethyl acetate fraction (YMS-EA) was the most potent fraction. YMS-EA also attenuated the hydrogen peroxide- or methylglyoxal-induced induction of reactive oxygen species, reduction of cell viability, and inhibition of cell proliferation. However, YMS-EA was unable to prevent hydrogen peroxide-induced apoptosis or advanced glycation end-products-induced toxicity. Under hyperglycemic conditions, YMS-EA effectively reduced ROS levels, improved mRNA expression of insulin, glucokinase, and PDX-1, and enhanced glucose-stimulated insulin secretion. The similarity of bioactivities among apigenin, luteolin, and YMS-EA indicated that dual activities of YMS-EA might be derived from those compounds. Conclusions: We concluded that YMS-EA fraction could be developed as a preventive food agent against the glucotoxicity to β-cells in Type 2 diabetes.

AB - Background: In this study, we aimed to develop a Stigmata Maydis (corn silk) fraction with dual bio-activities against oxidative stress and protein glycation to protect β-cells from diabetes-induced failure. Methods: Corn silk fractions were prepared by partition and chemically characterised by thin-layer chromatography. Free radical scavenging assay, glycation assay, and cell-based viability test (neutral red) were employed to decide the best fraction. Cell death analysis was executed by annexin V/ Propidium iodide staining. Cell proliferation was measured by WST-1. Finally, β-cell function was evaluated by β-cell marker gene expression (RT-PCR) and acute insulin secretion test. Results: Four corn silk fractions were prepared from an ethanolic crude extract of corn silk. In vitro assays indicate ethyl acetate fraction (YMS-EA) was the most potent fraction. YMS-EA also attenuated the hydrogen peroxide- or methylglyoxal-induced induction of reactive oxygen species, reduction of cell viability, and inhibition of cell proliferation. However, YMS-EA was unable to prevent hydrogen peroxide-induced apoptosis or advanced glycation end-products-induced toxicity. Under hyperglycemic conditions, YMS-EA effectively reduced ROS levels, improved mRNA expression of insulin, glucokinase, and PDX-1, and enhanced glucose-stimulated insulin secretion. The similarity of bioactivities among apigenin, luteolin, and YMS-EA indicated that dual activities of YMS-EA might be derived from those compounds. Conclusions: We concluded that YMS-EA fraction could be developed as a preventive food agent against the glucotoxicity to β-cells in Type 2 diabetes.

KW - Advanced glycation end products

KW - Glucotoxicity

KW - Methylglyoxal

KW - Reactive oxygen species

KW - Stigmata Maydis (corn silk)

KW - β-cell failure

UR - http://www.scopus.com/inward/record.url?scp=85009252997&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85009252997&partnerID=8YFLogxK

U2 - 10.1186/s12906-016-1382-8

DO - 10.1186/s12906-016-1382-8

M3 - Article

C2 - 27809830

AN - SCOPUS:85009252997

VL - 16

JO - BMC Complementary and Alternative Medicine

JF - BMC Complementary and Alternative Medicine

SN - 1472-6882

IS - 1

M1 - 432

ER -