The emerging role of SOX2 in cell proliferation and survival and its crosstalk with oncogenic signaling in lung cancer

Yu Ting Chou, Chih Chan Lee, Shih Hsin Hsiao, Sey En Lin, Sheng Chieh Lin, Chih Hung Chung, Chi Hsiu Chung, Yu Rong Kao, Yuan Hung Wang, Chien Tsun Chen, Yau Huei Wei, Cheng Wen Wu

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73 Citations (Scopus)


Tumor cells have long been observed to share several biological characteristics with normal stem/progenitor cells; however, the oncogenic mechanisms underlying the lung stem/progenitor cell signaling remain elusive. Here, we report that SOX2, a self-renewal factor in lung stem/progenitor cells, is highly expressed in a subclass of lung cancer cells, the proliferation, survival, and chemoresistance of which are dependent on SOX2 signaling. Overexpression of SOX2 promotes oncogenic phenotypes in lung cancer cells; knockdown of SOX2 attenuated cell proliferation. We observed that SOX2 increased the expression of epidermal growth factor receptor (EGFR), and EGFR activation further upregulated SOX2 levels, forming a positive feedback loop. SOX2 expression promoted chemoresistance, and silencing of SOX2 perturbed mitochondrial function, causing marked apoptosis and autophagy. SOX2 induced BCL2L1, the ectopic expression of which rescued the effects of SOX2 silencing on apoptosis, autophagy, and mitochondrial function. SOX2 promoted tumor formation, along with increased cell proliferation in a xenograft mouse model. SOX2 expression is associated with poor prognosis in lung cancer patients; moreover, SOX2, EGFR, and BCL2L1 expression levels were significantly correlated in lung tumors. Our findings support the emerging role of SOX2 in cell proliferation and survival by eliciting oncogenic EGFR and BCL2L1 signaling with potential applications as a prognosis marker and a therapeutic target in lung cancer.

Original languageEnglish
Pages (from-to)2607-2619
Number of pages13
JournalStem Cells
Issue number12
Publication statusPublished - Dec 2013



  • Apoptosis
  • Autophagy
  • Cancer stem cells
  • Lung cancer
  • Oncogenic pathway
  • Stem cell signaling

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine
  • Medicine(all)

Cite this

Chou, Y. T., Lee, C. C., Hsiao, S. H., Lin, S. E., Lin, S. C., Chung, C. H., Chung, C. H., Kao, Y. R., Wang, Y. H., Chen, C. T., Wei, Y. H., & Wu, C. W. (2013). The emerging role of SOX2 in cell proliferation and survival and its crosstalk with oncogenic signaling in lung cancer. Stem Cells, 31(12), 2607-2619.