The elevation of l-3-hydroxybutyrate concentrations in the serum of aristolochic acid-treated mice

Chien Ming Chen, Tzu Chuan Huang, Jen Ai Lee

Research output: Contribution to journalArticle


The concentrations of L-3-hydroxybutyrate (L-3HB) in the serum of aristolochic acid (AA)-treated mice were determined by column-switching high-performance liquid chromatography (HPLC). The samples were derivatized with 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ) and determined fluorimetrically. Total 3-HB derivatives in serum were separated by an ODS column and then introduced to a sample loop for chiral separation by two tandem CHIRALCEL OD-RH columns. The results showed that the concentration of L-3HB in the serum of normal mice was 0.81 ± 0.15 μM compared to that of AA-treated mice with 4.33 ± 2.81 μM after 3 days and 8.65 ± 2.60 μM after 5 days of AA administration (p

Original languageEnglish
Pages (from-to)25-30
Number of pages6
JournalInternational Journal of Bio-Science and Bio-Technology
Issue number1
Publication statusPublished - Mar 2010



  • Column-switching HPLC
  • L-3-hydroxybutyrate
  • Renal damage

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biotechnology
  • Bioengineering
  • Artificial Intelligence

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