The effects of negative pressure treatment on the extracellular matrix gene expression and protein production of fibroblasts

Pei Yu Chen, Chih Chin Hsu, Kai Chiang Yang, Chang Chin Wu, Chung Li Wang

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4 Citations (Scopus)


Although negative pressure wound therapy (NPWT) has been suggested for the management of acute and chronic wounds, the underlying mechanism of NPWT on the cellular level is not fully understood. Using a primary fibroblast culture model with controlled negative pressure, the cellular responses to the negative pressure was investigated. The present results showed that cell proliferation of fibroblasts was not affected under negative pressure. The mRNA expressions of collagen type I, collagen type III, and fibronectin were down-regulated when cells treated with negative pressure for once only. However, those mRNA expressions were up-regulated when cells subjected to periodical negative pressure consecutively for 5 days. The protein production of TGF-β1 was enhanced, while the TIMP-1, decorin, and IL-6 were not influenced. The pro-MMP-9 was not stimulated when fibroblasts subjected to negative pressure. The regulations of extracellular matrix production in fibroblasts provides an insight into the cellular mechanisms of NPWT.

Original languageEnglish
Article number10458
Pages (from-to)1662-1668
Number of pages7
JournalProcess Biochemistry
Issue number10
Publication statusPublished - Oct 1 2015



  • Extracellular matrix
  • Fibroblast
  • Negative pressure wound therapy
  • Wound healing

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Bioengineering

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