維生素K3單獨或併用游離輻射對人類肝癌細胞的影響

Translated title of the contribution: The Effects of Menadione Alone or Combined with Ionizing Radiation on Human Hepatoma Cells

黃正仲, 陳惠娥, 曾婉芳, 林冠如, 鄧文炳, 林芳仁

Research output: Contribution to journalArticle

Abstract

Purpose: To study the killing effects of menadione alone or combined with ionizing radiation on human hepatoma Hep3B cell.
Materials and Methods: log-phase Hep3B cells were treated with menadione, ionizing radiation or the combination of both agents. Three protocols for combined treatments: first, cells were pre-treated with 35 µM menadione for 3 hours then irradiated; second, cells were irradiated followed by the menadione treatment; third, cells were concurrently treated with both agents.
Results: The menadione inhibited the Hep3B tumor cells growth, the IC50 was around 60 μM. The intracellular reduced glutathione was significantly decreased after menadione treatment for 3 hours. After combined treatment most surviving fractions were less than 15%.
Conclusions: Both 'pre-irradiation followed by menadione' and 'concurrent treatment' have synergistic effect. However, 'pre-treatment of menadione followed by irradiation' only has the addition effect. This finding may be useful for clinical application of menadione when combined with ionizing radiation to treat cancers.
Original languageTraditional Chinese
Pages (from-to)101-109
Number of pages9
Journal放射治療與腫瘤學
Volume5
Issue number2
DOIs
Publication statusPublished - 1998
Externally publishedYes

    Fingerprint

Keywords

  • Menadione
  • Hep3B hepatoma cell
  • Combined treatment
  • Synergistic effect

Cite this