The effects of ferulic acid on nucleus pulposus cells under hydrogen peroxide-induced oxidative stress

Yung Hsin Cheng, Shu Hua Yang, Kai Chiang Yang, Moon Pei Chen, Feng Huei Lin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The degeneration of intervertebral disc (IVD) could be divided into 5 stages. In the first and second stages, there are no significant symptoms but could be traced by MRI or CT-scan. Generally, no aggressive treatment would be processed in the clinics. Reactive oxygen species (ROS) has been overproduced in degenerative IVD that could be associated with apoptosis of NP cells and degradation of ECM. Ferulic acid (FA) has been reported an excellent antioxidant and relatively stable in air. The study was aimed to evaluate the therapeutic effects of FA on hydrogen peroxide-induced oxidative stress NP cells and the potential for early treatment in IVD degeneration. The results showed that 500 μM of FA might be the threshold dose to treat NP cells without cytotoxicity. Post-treatment of FA on hydrogen peroxide-induced oxidative stress NP cells significantly up regulated the expression of aggrecan, type II collagen and BMP-7 and down regulated the expression of MMP-3 in mRNA level. Post-treatment of FA on hydrogen peroxide-induced oxidative stress NP cells could restore the production of sulfated-GAGs and inhibit the apoptosis caused by hydrogen peroxide. From the results of the study, FA could be used as a therapeutic agent for NP regeneration in the very near future.

Original languageEnglish
Pages (from-to)1670-1677
Number of pages8
JournalProcess Biochemistry
Volume46
Issue number8
DOIs
Publication statusPublished - Aug 2011
Externally publishedYes

Fingerprint

ferulic acid
Oxidative stress
Hydrogen peroxide
Hydrogen Peroxide
Oxidative Stress
Intervertebral Disc Degeneration
Acids
Cell death
Bone Morphogenetic Protein 7
Apoptosis
Military electronic countermeasures
Aggrecans
Collagen Type II
Computerized tomography
Therapeutic Uses
Cytotoxicity
Antioxidants
Matrix Metalloproteinases
Collagen
Nucleus Pulposus

Keywords

  • Antioxidant
  • Apoptosis
  • Ferulic acid
  • Intervertebral disc
  • Nucleus pulposus
  • Oxidative stress

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

The effects of ferulic acid on nucleus pulposus cells under hydrogen peroxide-induced oxidative stress. / Cheng, Yung Hsin; Yang, Shu Hua; Yang, Kai Chiang; Chen, Moon Pei; Lin, Feng Huei.

In: Process Biochemistry, Vol. 46, No. 8, 08.2011, p. 1670-1677.

Research output: Contribution to journalArticle

Cheng, Yung Hsin ; Yang, Shu Hua ; Yang, Kai Chiang ; Chen, Moon Pei ; Lin, Feng Huei. / The effects of ferulic acid on nucleus pulposus cells under hydrogen peroxide-induced oxidative stress. In: Process Biochemistry. 2011 ; Vol. 46, No. 8. pp. 1670-1677.
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