The effects of catechin on superoxide dismutase activity and its gene expression in pheochromocytoma cells

Jyh Ming Chow, Ju Chi Liu, Yi Jen Chen, Ming Hsiung Hsieh, Pai Feng Kao, Juei Jang Cheng, Paul Chan

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background. Overexpression of manganese superoxide dismutase (MnSOD) cDNA via plasmid transfection leads to growth inhibition in vitro and in vivo in various human cancers. Polyphenolic compounds such as catechin isolated from tea bush Camellia sinensis has been shown to have anticancer effect in vitro. This study evaluated the effect of catechin on the MnSOD activity and mRNA level of pheochromocytoma cells (PC-12). Methods. PC-12 cells were incubated with different concentrations of catechin at short-term (2 days) and long-term (7 days) in Dulbecco modified Eagle medium. The activity of superoxide dismutase (SOD) was measured and its mRNA was assayed by Northern blotting. Results. After incubation for 2 days, catechin slightly but significantly increased the activity of copper/zinc superoxide dismutase (CuZnSOD). However, it did not show any significant effect at 7 days. The MnSOD activity showed significant changes in both short-term and long-term. The amount of mRNA also showed similar changes. Conclusions. Catechin is a natural antioxidant which has been shown to have antitumor effect in basic and epidemiological studies. The present data suggest that catechin can increase MnSOD gene expression in PC-12, which might have beneficial effect in tumor prevention.

Original languageEnglish
Pages (from-to)138-143
Number of pages6
JournalChinese Medical Journal (Taipei)
Volume65
Issue number4
Publication statusPublished - 2002

Fingerprint

Catechin
Pheochromocytoma
Superoxide Dismutase
Gene Expression
Messenger RNA
Camellia sinensis
Eagles
Tea
Northern Blotting
Transfection
Zinc
Copper
Epidemiologic Studies
Neoplasms
Plasmids
Complementary DNA
Antioxidants
Growth

Keywords

  • Catechin
  • Manganese superoxide dismutase
  • mRNA
  • Pheochromocytoma cells

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "The effects of catechin on superoxide dismutase activity and its gene expression in pheochromocytoma cells",
abstract = "Background. Overexpression of manganese superoxide dismutase (MnSOD) cDNA via plasmid transfection leads to growth inhibition in vitro and in vivo in various human cancers. Polyphenolic compounds such as catechin isolated from tea bush Camellia sinensis has been shown to have anticancer effect in vitro. This study evaluated the effect of catechin on the MnSOD activity and mRNA level of pheochromocytoma cells (PC-12). Methods. PC-12 cells were incubated with different concentrations of catechin at short-term (2 days) and long-term (7 days) in Dulbecco modified Eagle medium. The activity of superoxide dismutase (SOD) was measured and its mRNA was assayed by Northern blotting. Results. After incubation for 2 days, catechin slightly but significantly increased the activity of copper/zinc superoxide dismutase (CuZnSOD). However, it did not show any significant effect at 7 days. The MnSOD activity showed significant changes in both short-term and long-term. The amount of mRNA also showed similar changes. Conclusions. Catechin is a natural antioxidant which has been shown to have antitumor effect in basic and epidemiological studies. The present data suggest that catechin can increase MnSOD gene expression in PC-12, which might have beneficial effect in tumor prevention.",
keywords = "Catechin, Manganese superoxide dismutase, mRNA, Pheochromocytoma cells",
author = "Chow, {Jyh Ming} and Liu, {Ju Chi} and Chen, {Yi Jen} and Hsieh, {Ming Hsiung} and Kao, {Pai Feng} and Cheng, {Juei Jang} and Paul Chan",
year = "2002",
language = "English",
volume = "65",
pages = "138--143",
journal = "Journal of the Chinese Medical Association",
issn = "1726-4901",
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number = "4",

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TY - JOUR

T1 - The effects of catechin on superoxide dismutase activity and its gene expression in pheochromocytoma cells

AU - Chow, Jyh Ming

AU - Liu, Ju Chi

AU - Chen, Yi Jen

AU - Hsieh, Ming Hsiung

AU - Kao, Pai Feng

AU - Cheng, Juei Jang

AU - Chan, Paul

PY - 2002

Y1 - 2002

N2 - Background. Overexpression of manganese superoxide dismutase (MnSOD) cDNA via plasmid transfection leads to growth inhibition in vitro and in vivo in various human cancers. Polyphenolic compounds such as catechin isolated from tea bush Camellia sinensis has been shown to have anticancer effect in vitro. This study evaluated the effect of catechin on the MnSOD activity and mRNA level of pheochromocytoma cells (PC-12). Methods. PC-12 cells were incubated with different concentrations of catechin at short-term (2 days) and long-term (7 days) in Dulbecco modified Eagle medium. The activity of superoxide dismutase (SOD) was measured and its mRNA was assayed by Northern blotting. Results. After incubation for 2 days, catechin slightly but significantly increased the activity of copper/zinc superoxide dismutase (CuZnSOD). However, it did not show any significant effect at 7 days. The MnSOD activity showed significant changes in both short-term and long-term. The amount of mRNA also showed similar changes. Conclusions. Catechin is a natural antioxidant which has been shown to have antitumor effect in basic and epidemiological studies. The present data suggest that catechin can increase MnSOD gene expression in PC-12, which might have beneficial effect in tumor prevention.

AB - Background. Overexpression of manganese superoxide dismutase (MnSOD) cDNA via plasmid transfection leads to growth inhibition in vitro and in vivo in various human cancers. Polyphenolic compounds such as catechin isolated from tea bush Camellia sinensis has been shown to have anticancer effect in vitro. This study evaluated the effect of catechin on the MnSOD activity and mRNA level of pheochromocytoma cells (PC-12). Methods. PC-12 cells were incubated with different concentrations of catechin at short-term (2 days) and long-term (7 days) in Dulbecco modified Eagle medium. The activity of superoxide dismutase (SOD) was measured and its mRNA was assayed by Northern blotting. Results. After incubation for 2 days, catechin slightly but significantly increased the activity of copper/zinc superoxide dismutase (CuZnSOD). However, it did not show any significant effect at 7 days. The MnSOD activity showed significant changes in both short-term and long-term. The amount of mRNA also showed similar changes. Conclusions. Catechin is a natural antioxidant which has been shown to have antitumor effect in basic and epidemiological studies. The present data suggest that catechin can increase MnSOD gene expression in PC-12, which might have beneficial effect in tumor prevention.

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