Abstract

The function of type II collagen in cartilage is well documented and its importance for long bone development has been implicated. However, the involvement of type II collagen in bone marrow derived mesenchymal stem cell (BMSC) osteogenesis has not been well investigated. This study elucidated the pivotal role of type II collagen in BMSC osteogenesis and its potential application to bone healing. Type II collagen-coated surface was found to accelerate calcium deposition, and the interaction of osteogenic medium-induced BMSCs with type II collagen-coated surface was mainly mediated through integrin α2β1. Exogenous type II collagen directly activated FAK-JNK signaling and resulted in the phosphorylation of RUNX2. In a segmental defect model in rats, type II collagen-HA/TCP-implanted rats showed significant callus formation at the reunion site, and a higher SFI (sciatic function index) scoring as comparing to other groups were also observed at 7, 14, and 21 day post-surgery. Collectively, type II collagen serves as a better modulator during early osteogenic differentiation of BMSCs by facilitating RUNX2 activation through integrin α2β1-FAK-JNK signaling axis, and enhance bone defect repair through an endochondral ossification-like process. These results advance our understanding about the cartilaginous ECM-BMSC interaction, and provide perspective for bone defect repair strategies.

Original languageEnglish
Pages (from-to)2680-2691
Number of pages12
JournalBiomaterials
Volume35
Issue number9
DOIs
Publication statusPublished - Mar 2014

Fingerprint

Collagen Type II
Collagen
Bone
Repair
Bone and Bones
Defects
Stem cells
Mesenchymal Stromal Cells
Osteogenesis
Bone Marrow
Integrins
Rats
Reunion
Military electronic countermeasures
Phosphorylation
Bone Development
Bony Callus
Cartilage
Ambulatory Surgical Procedures
Cell Communication

Keywords

  • Bone healing
  • Cell adhesion
  • Integrin signaling
  • Mesenchymal stem cell
  • Osteogenesis
  • Type II collagen

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

The effect of type II collagen on MSC osteogenic differentiation and bone defect repair. / Chiu, Li Hsuan; Lai, Wen Fu T; Chang, Shwu Fen; Wong, Chin Chean; Fan, Cheng Yu; Fang, Chia Lang; Tsai, Yu-Hui.

In: Biomaterials, Vol. 35, No. 9, 03.2014, p. 2680-2691.

Research output: Contribution to journalArticle

Chiu, Li Hsuan ; Lai, Wen Fu T ; Chang, Shwu Fen ; Wong, Chin Chean ; Fan, Cheng Yu ; Fang, Chia Lang ; Tsai, Yu-Hui. / The effect of type II collagen on MSC osteogenic differentiation and bone defect repair. In: Biomaterials. 2014 ; Vol. 35, No. 9. pp. 2680-2691.
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