The effect of surface charge on the uptake and biological function of mesoporous silica nanoparticles in 3T3-L1 cells and human mesenchymal stem cells

Tsai Hua Chung, Si Han Wu, Ming Yao, Chen Wen Lu, Yu Shen Lin, Yann Hung, Chung Yuan Mou, Yao Chang Chen, Dong Ming Huang

Research output: Contribution to journalArticle

461 Citations (Scopus)

Abstract

Cellular uptake of nanoparticles for stem cell labeling/tracking is considered as the most promising method. Recently mesoporous silica nanoparticles (MSNs) are emerging as an idea agent for efficient stem cell labeling. The objective of this study was to evaluate the effect of surface charge on the highly efficient cellular uptake and in vitro cytotoxicity of MSNs in human mesenchymal stem cells (hMSCs). The surface charge was varied by the degree of surface modification with N-trimethoxysilylpropyl-N,N,N-trimethylammonium chloride and the uptake of MSNs was detected by flow cytometry. 3T3-L1 cells were also used to compare the uptake behavior of MSNs between cell types. A clear correlation of positive surface charge and the number of fluorescence-labeled cells was mainly observed in 3T3-L1 cells. In both cells, uptake of unmodified MSNs was inhibited by phenylarsine oxide (PAO) and cytochalasin D (Cyt D) suggesting a clathrin- and an actin-dependent endocytosis were involved. With strongly positive-charged MSNs, the inhibitory effects were observed in 3T3-L1 cells but not in hMSCs. Without regard to the surface charge, uptake of MSNs into both cells did not affect their viability, proliferation, and differentiation. Our results show that MSNs uptake by hMSCs can be regulated by a threshold of positive surface charge but also imply that the modulation of surface charge on MSNs uptake is specific to cell type.

Original languageEnglish
Pages (from-to)2959-2966
Number of pages8
JournalBiomaterials
Volume28
Issue number19
DOIs
Publication statusPublished - Jul 2007
Externally publishedYes

Fingerprint

3T3-L1 Cells
Surface charge
Stem cells
Mesenchymal Stromal Cells
Silicon Dioxide
Nanoparticles
Silica
Labeling
Stem Cells
Cell Tracking
Cytochalasin D
Clathrin
Flow cytometry
Cytotoxicity
Endocytosis
Surface treatment
Actins
Chlorides
Flow Cytometry
Fluorescence

Keywords

  • Biocompatibility
  • Cellular uptake
  • Mesenchymal stem cell
  • Nanoparticle
  • Silica
  • Surface modification

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

The effect of surface charge on the uptake and biological function of mesoporous silica nanoparticles in 3T3-L1 cells and human mesenchymal stem cells. / Chung, Tsai Hua; Wu, Si Han; Yao, Ming; Lu, Chen Wen; Lin, Yu Shen; Hung, Yann; Mou, Chung Yuan; Chen, Yao Chang; Huang, Dong Ming.

In: Biomaterials, Vol. 28, No. 19, 07.2007, p. 2959-2966.

Research output: Contribution to journalArticle

Chung, Tsai Hua ; Wu, Si Han ; Yao, Ming ; Lu, Chen Wen ; Lin, Yu Shen ; Hung, Yann ; Mou, Chung Yuan ; Chen, Yao Chang ; Huang, Dong Ming. / The effect of surface charge on the uptake and biological function of mesoporous silica nanoparticles in 3T3-L1 cells and human mesenchymal stem cells. In: Biomaterials. 2007 ; Vol. 28, No. 19. pp. 2959-2966.
@article{cf1f4b78dc3a465e89e8b14c71300001,
title = "The effect of surface charge on the uptake and biological function of mesoporous silica nanoparticles in 3T3-L1 cells and human mesenchymal stem cells",
abstract = "Cellular uptake of nanoparticles for stem cell labeling/tracking is considered as the most promising method. Recently mesoporous silica nanoparticles (MSNs) are emerging as an idea agent for efficient stem cell labeling. The objective of this study was to evaluate the effect of surface charge on the highly efficient cellular uptake and in vitro cytotoxicity of MSNs in human mesenchymal stem cells (hMSCs). The surface charge was varied by the degree of surface modification with N-trimethoxysilylpropyl-N,N,N-trimethylammonium chloride and the uptake of MSNs was detected by flow cytometry. 3T3-L1 cells were also used to compare the uptake behavior of MSNs between cell types. A clear correlation of positive surface charge and the number of fluorescence-labeled cells was mainly observed in 3T3-L1 cells. In both cells, uptake of unmodified MSNs was inhibited by phenylarsine oxide (PAO) and cytochalasin D (Cyt D) suggesting a clathrin- and an actin-dependent endocytosis were involved. With strongly positive-charged MSNs, the inhibitory effects were observed in 3T3-L1 cells but not in hMSCs. Without regard to the surface charge, uptake of MSNs into both cells did not affect their viability, proliferation, and differentiation. Our results show that MSNs uptake by hMSCs can be regulated by a threshold of positive surface charge but also imply that the modulation of surface charge on MSNs uptake is specific to cell type.",
keywords = "Biocompatibility, Cellular uptake, Mesenchymal stem cell, Nanoparticle, Silica, Surface modification",
author = "Chung, {Tsai Hua} and Wu, {Si Han} and Ming Yao and Lu, {Chen Wen} and Lin, {Yu Shen} and Yann Hung and Mou, {Chung Yuan} and Chen, {Yao Chang} and Huang, {Dong Ming}",
year = "2007",
month = "7",
doi = "10.1016/j.biomaterials.2007.03.006",
language = "English",
volume = "28",
pages = "2959--2966",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier Science Ltd",
number = "19",

}

TY - JOUR

T1 - The effect of surface charge on the uptake and biological function of mesoporous silica nanoparticles in 3T3-L1 cells and human mesenchymal stem cells

AU - Chung, Tsai Hua

AU - Wu, Si Han

AU - Yao, Ming

AU - Lu, Chen Wen

AU - Lin, Yu Shen

AU - Hung, Yann

AU - Mou, Chung Yuan

AU - Chen, Yao Chang

AU - Huang, Dong Ming

PY - 2007/7

Y1 - 2007/7

N2 - Cellular uptake of nanoparticles for stem cell labeling/tracking is considered as the most promising method. Recently mesoporous silica nanoparticles (MSNs) are emerging as an idea agent for efficient stem cell labeling. The objective of this study was to evaluate the effect of surface charge on the highly efficient cellular uptake and in vitro cytotoxicity of MSNs in human mesenchymal stem cells (hMSCs). The surface charge was varied by the degree of surface modification with N-trimethoxysilylpropyl-N,N,N-trimethylammonium chloride and the uptake of MSNs was detected by flow cytometry. 3T3-L1 cells were also used to compare the uptake behavior of MSNs between cell types. A clear correlation of positive surface charge and the number of fluorescence-labeled cells was mainly observed in 3T3-L1 cells. In both cells, uptake of unmodified MSNs was inhibited by phenylarsine oxide (PAO) and cytochalasin D (Cyt D) suggesting a clathrin- and an actin-dependent endocytosis were involved. With strongly positive-charged MSNs, the inhibitory effects were observed in 3T3-L1 cells but not in hMSCs. Without regard to the surface charge, uptake of MSNs into both cells did not affect their viability, proliferation, and differentiation. Our results show that MSNs uptake by hMSCs can be regulated by a threshold of positive surface charge but also imply that the modulation of surface charge on MSNs uptake is specific to cell type.

AB - Cellular uptake of nanoparticles for stem cell labeling/tracking is considered as the most promising method. Recently mesoporous silica nanoparticles (MSNs) are emerging as an idea agent for efficient stem cell labeling. The objective of this study was to evaluate the effect of surface charge on the highly efficient cellular uptake and in vitro cytotoxicity of MSNs in human mesenchymal stem cells (hMSCs). The surface charge was varied by the degree of surface modification with N-trimethoxysilylpropyl-N,N,N-trimethylammonium chloride and the uptake of MSNs was detected by flow cytometry. 3T3-L1 cells were also used to compare the uptake behavior of MSNs between cell types. A clear correlation of positive surface charge and the number of fluorescence-labeled cells was mainly observed in 3T3-L1 cells. In both cells, uptake of unmodified MSNs was inhibited by phenylarsine oxide (PAO) and cytochalasin D (Cyt D) suggesting a clathrin- and an actin-dependent endocytosis were involved. With strongly positive-charged MSNs, the inhibitory effects were observed in 3T3-L1 cells but not in hMSCs. Without regard to the surface charge, uptake of MSNs into both cells did not affect their viability, proliferation, and differentiation. Our results show that MSNs uptake by hMSCs can be regulated by a threshold of positive surface charge but also imply that the modulation of surface charge on MSNs uptake is specific to cell type.

KW - Biocompatibility

KW - Cellular uptake

KW - Mesenchymal stem cell

KW - Nanoparticle

KW - Silica

KW - Surface modification

UR - http://www.scopus.com/inward/record.url?scp=34147144905&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34147144905&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2007.03.006

DO - 10.1016/j.biomaterials.2007.03.006

M3 - Article

C2 - 17397919

AN - SCOPUS:34147144905

VL - 28

SP - 2959

EP - 2966

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 19

ER -