The effect of hyperbaric oxygen and air on cartilage tissue engineering

Juin-Hong Cherng, Shun-Cheng Chang, Shyi-Gen Chen, Ming-Lun Hsu, Po-Da Hong, Shou-Chen Teng, Yi-Hsin Chan, Chih-Hsin Wang, Tim-Mo Chen, Niann-Tzyy Dai

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


There is an urgent need to develop tissue-engineered cartilage for patients experiencing joint malfunction due to insufficient self-repairing capacity of articular cartilage. The aim of this research was to explore the effect of hyperbaric oxygen and air on tissue-engineered cartilage formation from human adipose-derived stem cells seeding on the gelatin/polycaprolactone biocomposites. The results of histological analyses indicate that under hyperbaric oxygen and air stimulation, the cell number of chondrocytes in cartilage matrix was not significantly increased, but the 1,9-dimethylmethylene blue assay showed that the glycosaminoglycans syntheses markedly increased compared to the control group. In quantification real-time polymerase chain reaction results, the chondrogenic-specific gene expression of SOX9, aggrecan, and COL2A1 were compared respectively. Within the limitation of this study, it was concluded that 2.5 atmosphere absolute oxygen and air may provide a stress environment to help cartilage tissue engineering development. Copyright © 2012 by Lippincott Williams & Wilkins.
Original languageEnglish
Pages (from-to)650-655
Number of pages6
JournalAnnals of Plastic Surgery
Issue number6
Publication statusPublished - 2012
Externally publishedYes


  • atmosphere absolute (ATA)
  • cartilage tissue engineering
  • gelatin/polycaprolactone
  • glycosaminoglycan
  • human adipose-derived stem cells (hASCs)
  • hyperbaric oxygen
  • adipocyte
  • air
  • analysis of variance
  • article
  • cartilage
  • cell differentiation
  • cell survival
  • chondrogenesis
  • cytology
  • extracellular matrix
  • genetics
  • human
  • methodology
  • stem cell
  • tissue engineering
  • Adipocytes
  • Air
  • Analysis of Variance
  • Cartilage
  • Cell Differentiation
  • Cell Survival
  • Chondrogenesis
  • Extracellular Matrix
  • Humans
  • Hyperbaric Oxygenation
  • Stem Cells
  • Tissue Engineering


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