The effect of hyperbaric oxygen and air on cartilage tissue engineering

Juin-Hong Cherng, Shun-Cheng Chang, Shyi-Gen Chen, Ming-Lun Hsu, Po-Da Hong, Shou-Chen Teng, Yi-Hsin Chan, Chih-Hsin Wang, Tim-Mo Chen, Niann-Tzyy Dai

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

There is an urgent need to develop tissue-engineered cartilage for patients experiencing joint malfunction due to insufficient self-repairing capacity of articular cartilage. The aim of this research was to explore the effect of hyperbaric oxygen and air on tissue-engineered cartilage formation from human adipose-derived stem cells seeding on the gelatin/polycaprolactone biocomposites. The results of histological analyses indicate that under hyperbaric oxygen and air stimulation, the cell number of chondrocytes in cartilage matrix was not significantly increased, but the 1,9-dimethylmethylene blue assay showed that the glycosaminoglycans syntheses markedly increased compared to the control group. In quantification real-time polymerase chain reaction results, the chondrogenic-specific gene expression of SOX9, aggrecan, and COL2A1 were compared respectively. Within the limitation of this study, it was concluded that 2.5 atmosphere absolute oxygen and air may provide a stress environment to help cartilage tissue engineering development. Copyright © 2012 by Lippincott Williams & Wilkins.
Original languageEnglish
Pages (from-to)650-655
Number of pages6
JournalAnnals of Plastic Surgery
Volume69
Issue number6
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Tissue Engineering
Cartilage
Air
Oxygen
Aggrecans
Articular Cartilage
Gelatin
Chondrocytes
Glycosaminoglycans
Atmosphere
Real-Time Polymerase Chain Reaction
Stem Cells
Cell Count
Joints
Gene Expression
Control Groups
Research

Keywords

  • atmosphere absolute (ATA)
  • cartilage tissue engineering
  • gelatin/polycaprolactone
  • glycosaminoglycan
  • human adipose-derived stem cells (hASCs)
  • hyperbaric oxygen
  • adipocyte
  • air
  • analysis of variance
  • article
  • cartilage
  • cell differentiation
  • cell survival
  • chondrogenesis
  • cytology
  • extracellular matrix
  • genetics
  • human
  • methodology
  • stem cell
  • tissue engineering
  • Adipocytes
  • Air
  • Analysis of Variance
  • Cartilage
  • Cell Differentiation
  • Cell Survival
  • Chondrogenesis
  • Extracellular Matrix
  • Humans
  • Hyperbaric Oxygenation
  • Stem Cells
  • Tissue Engineering

Cite this

Cherng, J-H., Chang, S-C., Chen, S-G., Hsu, M-L., Hong, P-D., Teng, S-C., ... Dai, N-T. (2012). The effect of hyperbaric oxygen and air on cartilage tissue engineering. Annals of Plastic Surgery, 69(6), 650-655. https://doi.org/10.1097/SAP.0b013e3182745f95

The effect of hyperbaric oxygen and air on cartilage tissue engineering. / Cherng, Juin-Hong; Chang, Shun-Cheng; Chen, Shyi-Gen; Hsu, Ming-Lun; Hong, Po-Da; Teng, Shou-Chen; Chan, Yi-Hsin; Wang, Chih-Hsin; Chen, Tim-Mo; Dai, Niann-Tzyy.

In: Annals of Plastic Surgery, Vol. 69, No. 6, 2012, p. 650-655.

Research output: Contribution to journalArticle

Cherng, J-H, Chang, S-C, Chen, S-G, Hsu, M-L, Hong, P-D, Teng, S-C, Chan, Y-H, Wang, C-H, Chen, T-M & Dai, N-T 2012, 'The effect of hyperbaric oxygen and air on cartilage tissue engineering', Annals of Plastic Surgery, vol. 69, no. 6, pp. 650-655. https://doi.org/10.1097/SAP.0b013e3182745f95
Cherng, Juin-Hong ; Chang, Shun-Cheng ; Chen, Shyi-Gen ; Hsu, Ming-Lun ; Hong, Po-Da ; Teng, Shou-Chen ; Chan, Yi-Hsin ; Wang, Chih-Hsin ; Chen, Tim-Mo ; Dai, Niann-Tzyy. / The effect of hyperbaric oxygen and air on cartilage tissue engineering. In: Annals of Plastic Surgery. 2012 ; Vol. 69, No. 6. pp. 650-655.
@article{75c1e49949aa49b3abaa1ffe2d0b65d4,
title = "The effect of hyperbaric oxygen and air on cartilage tissue engineering",
abstract = "There is an urgent need to develop tissue-engineered cartilage for patients experiencing joint malfunction due to insufficient self-repairing capacity of articular cartilage. The aim of this research was to explore the effect of hyperbaric oxygen and air on tissue-engineered cartilage formation from human adipose-derived stem cells seeding on the gelatin/polycaprolactone biocomposites. The results of histological analyses indicate that under hyperbaric oxygen and air stimulation, the cell number of chondrocytes in cartilage matrix was not significantly increased, but the 1,9-dimethylmethylene blue assay showed that the glycosaminoglycans syntheses markedly increased compared to the control group. In quantification real-time polymerase chain reaction results, the chondrogenic-specific gene expression of SOX9, aggrecan, and COL2A1 were compared respectively. Within the limitation of this study, it was concluded that 2.5 atmosphere absolute oxygen and air may provide a stress environment to help cartilage tissue engineering development. Copyright {\circledC} 2012 by Lippincott Williams & Wilkins.",
keywords = "atmosphere absolute (ATA), cartilage tissue engineering, gelatin/polycaprolactone, glycosaminoglycan, human adipose-derived stem cells (hASCs), hyperbaric oxygen, adipocyte, air, analysis of variance, article, cartilage, cell differentiation, cell survival, chondrogenesis, cytology, extracellular matrix, genetics, human, methodology, stem cell, tissue engineering, Adipocytes, Air, Analysis of Variance, Cartilage, Cell Differentiation, Cell Survival, Chondrogenesis, Extracellular Matrix, Humans, Hyperbaric Oxygenation, Stem Cells, Tissue Engineering",
author = "Juin-Hong Cherng and Shun-Cheng Chang and Shyi-Gen Chen and Ming-Lun Hsu and Po-Da Hong and Shou-Chen Teng and Yi-Hsin Chan and Chih-Hsin Wang and Tim-Mo Chen and Niann-Tzyy Dai",
note = "被引用次數:6 Export Date: 21 March 2016 CODEN: APCSD 通訊地址: Dai, N.-T.; Department of Plastic and Reconstructive Surgery, Tri-Service General Hospital, No. 325, Sec. 2, Chenggong Rd, Neihu District, Taipei City 114, Taiwan; 電子郵件: niantzyy@ms17.hinet.net 參考文獻: Johnson, L.L., Arthroscopic abrasion arthroplasty historical and pathologic perspective: Present status (1986) Arthroscopy., 2, pp. 54-69; Steadman, J.R., Rodkey, W.G., Briggs, K.K., Microfracture to treat full-thickness chondral defects: Surgical technique, rehabilitation, and outcomes (2002) J Knee Surg., 15, pp. 170-176; Gross, A.E., Aubin, P., Cheah, H.K., A fresh osteochondral allograft alternative (2002) J Arthroplasty., 17, pp. 50-53; Ghazavi, M.T., Pritzker, K.P., Davis, A.M., Fresh osteochondral allografts for post-traumatic osteochondral defects of the knee (1997) J Bone Joint Surg Br., 79 B, pp. 1008-1013; Smith, G.D., Richardson, J.B., Brittberg, M., Autologous chondrocyte implantation and osteochondral cylinder transplantation in cartilage repair of the knee joint (2003) J Bone Joint Surg Am., 85 A, pp. 2487-2488; Outerbridge, H.K., Outerbridge, A.R., Outerbridge, R.E., The use of a lateral patellar autologous graft for the repair of a large osteochondral defect in the knee (1995) J Bone Joint Surg Am., 77, pp. 65-72; Tuli, R., Li, W.-J., Tuan, R., Current state of cartilage tissue engineering (2003) Arthritis Res Ther., 5, pp. 235-238; Nerem, R.M., Sambanis, A., Tissue engineering: From biology to biological substitutes (1995) Tissue Eng., 1, pp. 3-13; Komarek, J., Valis, P., Repko, M., Treatment of deep cartilage defects of the knee with autologous chondrocyte transplantation: Long-term results (2010) Acta Chir Orthop Traumatol Cech., 77, pp. 291-295; Chou, C.-H., Cheng, W.-T., Lin, C.-C., TGF-beta1 immobilized tri-co-polymer for articular cartilage tissue engineering (2006) J Biomed Mater Res B Appl Biomater., 77, pp. 338-348; Pieper, J.S., Hafmans, T., Veerkamp, J.H., Development of tailor-made collagen-glycosaminoglycan matrices: EDC/NHS crosslinking, and ultrastructural aspects (2000) Biomaterials., 21, pp. 581-593; Pieper, J.S., Oosterhof, Dijkstra, P.J., Preparation and characterization of porous crosslinked collagenous matrices containing bioavailable chondroitin sulphate (1999) Biomaterials., pp. 847-858; Park, S.N., Park, J.C., Kim, H.O., Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linking (2002) Biomaterials., 23, pp. 1205-1212; Lee, J.-E., Kim, K.-E., Kwon, I.-C., Effects of the controlled-released TGF-A1 from chitosan microspheres on chondrocytes cultured in a collagen/chitosan/glycosaminoglycan scaffold (2004) Biomaterials., 25, pp. 4163-4173; Chang, J.C., Hsu, S.H., Chen, D.C., The promotion of chondrogenesis in adipose-derived adult stem cells by an RGD-chimeric protein in 3D alginate culture (2009) Biomaterials., 30, pp. 6265-6275; Chang, C.-H., Liu, H.-C., Lin, C.-C., Gelatin-chondroitin-hyaluronan tri-copolymer scaffold for cartilage tissue engineering (2003) Biomaterials., 24, pp. 4853-4858; Concaro, S., Nicklasson, E., Ellowsson, L., Effect of cell seeding concentration on the quality of tissue engineered constructs loaded with adult human articular chondrocytes (2008) J Tissue Eng Regen Med., 2, pp. 14-21; Fan, H.-B., Hu, Y.-Y., Zhang, C.-L., Cartilage regeneration using mesenchymal stem cells and a PLGA-gelatin/chondroitin/hyaluronate hybrid scaffold (2006) Biomaterials., 27, pp. 4573-4580; Kim, H.J., Lee, J.H., Gun, G.I., Chondrogenesis using mesenchymal stem cells and PCL scaffolds (2010) J Biomed Mater Res A., 92 A, pp. 659-666; Izquierdo, R., Garcia-Giralt, N., Rodriguez, M.T., Biodegradable PCL scaffolds with an interconnected spherical pore network for tissue engineering (2008) J Biomed Mater Res A., 85 A, pp. 25-35; Guilak, F., Estes, B., Diekman, B., 2010 Nicolas Andry Award: Multipotent adult stem cells from adipose tissue for musculoskeletal tissue engineering (2010) Clin Orthop Relat Res., 468, pp. 2530-2540; Lee, R.H., Kim, B., Choi, I., Characterization and expression analysis of mesenchymal stem cells from human bone marrow and adipose tissue (2004) Cell Physiol Biochem., 14, pp. 311-324; Wagner, W., Wein, F., Seckinger, A., Comparative characteristics of mes-enchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood (2005) Exp Hematol., 33, pp. 1402-1416; Dicker, A., Le Blanc, K., {\AA}str{\"o}m, G., Functional studies of mesenchymal stem cells derived from adult human adipose tissue (2005) Exp Cell Res., 308, pp. 283-290; Zuk, P.A., Zhu, M., Mizuno, H., Multilineage cells from human adipose tissue: Implications for cell-based therapies (2001) Tissue Eng., 7, pp. 211-228; Quintana, L., Zur Nieden, N.I., Semino, C.E., Morphogenetic and regulatory mechanisms during developmental chondrogenesis: New paradigms for cartilage tissue engineering (2009) Tissue Eng Part B Rev., 15, pp. 29-41; Chen, A.C., Lee, M.S., Lin, S.S., Augmentation of osteochondral repair with hyperbaric oxygenation: A rabbit study (2010) J Orthop Surg Res, 5, p. 91; Nilsson, P., Albrektsson, T., Granstrom, G., The effect of hyperbaric oxygen treatment on bone regeneration: An experimental study using the bone harvest chamber in the rabbit (1998) Int J Oral Maxillofac Implants., 3, pp. 43-48; Roberts, G.P., Harding, K.G., Stimulation of glycosaminoglycan synthesis in cultured fibroblasts by hyperbaric oxygen (1994) Br J Dermatol., 131, pp. 630-633; Kang, T.S., Gorti, G.K., Quan, S.Y., Effect of hyperbaric oxygen on the growth factor profile of fibroblasts (2004) Arch Facial Plast Surg., 6, pp. 31-35; Natesan, S., Baer, D.G., Walters, T.J., Adipose-derived stem cell delivery into collagen gels using chitosan microspheres (2010) Tissue Eng Part A., 16, pp. 1369-1384; Rigotti, G., Marchi, A., Gali{\`e}, M., Clinical treatment of radiotherapy tissue damage by lipoaspirate transplant: A healing process mediated by adipose-derived adult stem cells (2007) Plast Reconstr Surg., 119, pp. 1409-1422; Heng, B.C., Cao, T., Lee, E.H., Directing stem cell differentiation into the chon-drogenic lineage in vitro (2004) Stem Cells., 22, pp. 1152-1167; Hsieh, C.P., Chiou, Y.L., Lin, C.Y., Hyperbaric oxygen-stimulated proliferation and growth of osteoblasts may be mediated through the FGF-2/MEK/ERK 1/2/NF-kappaB and PKC/JNK pathways (2010) Connect Tissue Res., 51, pp. 497-509; Ogawa, R., Mizuno, S., Murphy, G.F., The effect of hydrostatic pressure on three-dimensional chondroinduction of human adipose-derived stem cells (2009) Tissue Eng Part A., 15, pp. 2937-2945",
year = "2012",
doi = "10.1097/SAP.0b013e3182745f95",
language = "English",
volume = "69",
pages = "650--655",
journal = "Annals of Plastic Surgery",
issn = "0148-7043",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - The effect of hyperbaric oxygen and air on cartilage tissue engineering

AU - Cherng, Juin-Hong

AU - Chang, Shun-Cheng

AU - Chen, Shyi-Gen

AU - Hsu, Ming-Lun

AU - Hong, Po-Da

AU - Teng, Shou-Chen

AU - Chan, Yi-Hsin

AU - Wang, Chih-Hsin

AU - Chen, Tim-Mo

AU - Dai, Niann-Tzyy

N1 - 被引用次數:6 Export Date: 21 March 2016 CODEN: APCSD 通訊地址: Dai, N.-T.; Department of Plastic and Reconstructive Surgery, Tri-Service General Hospital, No. 325, Sec. 2, Chenggong Rd, Neihu District, Taipei City 114, Taiwan; 電子郵件: niantzyy@ms17.hinet.net 參考文獻: Johnson, L.L., Arthroscopic abrasion arthroplasty historical and pathologic perspective: Present status (1986) Arthroscopy., 2, pp. 54-69; Steadman, J.R., Rodkey, W.G., Briggs, K.K., Microfracture to treat full-thickness chondral defects: Surgical technique, rehabilitation, and outcomes (2002) J Knee Surg., 15, pp. 170-176; Gross, A.E., Aubin, P., Cheah, H.K., A fresh osteochondral allograft alternative (2002) J Arthroplasty., 17, pp. 50-53; Ghazavi, M.T., Pritzker, K.P., Davis, A.M., Fresh osteochondral allografts for post-traumatic osteochondral defects of the knee (1997) J Bone Joint Surg Br., 79 B, pp. 1008-1013; Smith, G.D., Richardson, J.B., Brittberg, M., Autologous chondrocyte implantation and osteochondral cylinder transplantation in cartilage repair of the knee joint (2003) J Bone Joint Surg Am., 85 A, pp. 2487-2488; Outerbridge, H.K., Outerbridge, A.R., Outerbridge, R.E., The use of a lateral patellar autologous graft for the repair of a large osteochondral defect in the knee (1995) J Bone Joint Surg Am., 77, pp. 65-72; Tuli, R., Li, W.-J., Tuan, R., Current state of cartilage tissue engineering (2003) Arthritis Res Ther., 5, pp. 235-238; Nerem, R.M., Sambanis, A., Tissue engineering: From biology to biological substitutes (1995) Tissue Eng., 1, pp. 3-13; Komarek, J., Valis, P., Repko, M., Treatment of deep cartilage defects of the knee with autologous chondrocyte transplantation: Long-term results (2010) Acta Chir Orthop Traumatol Cech., 77, pp. 291-295; Chou, C.-H., Cheng, W.-T., Lin, C.-C., TGF-beta1 immobilized tri-co-polymer for articular cartilage tissue engineering (2006) J Biomed Mater Res B Appl Biomater., 77, pp. 338-348; Pieper, J.S., Hafmans, T., Veerkamp, J.H., Development of tailor-made collagen-glycosaminoglycan matrices: EDC/NHS crosslinking, and ultrastructural aspects (2000) Biomaterials., 21, pp. 581-593; Pieper, J.S., Oosterhof, Dijkstra, P.J., Preparation and characterization of porous crosslinked collagenous matrices containing bioavailable chondroitin sulphate (1999) Biomaterials., pp. 847-858; Park, S.N., Park, J.C., Kim, H.O., Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linking (2002) Biomaterials., 23, pp. 1205-1212; Lee, J.-E., Kim, K.-E., Kwon, I.-C., Effects of the controlled-released TGF-A1 from chitosan microspheres on chondrocytes cultured in a collagen/chitosan/glycosaminoglycan scaffold (2004) Biomaterials., 25, pp. 4163-4173; Chang, J.C., Hsu, S.H., Chen, D.C., The promotion of chondrogenesis in adipose-derived adult stem cells by an RGD-chimeric protein in 3D alginate culture (2009) Biomaterials., 30, pp. 6265-6275; Chang, C.-H., Liu, H.-C., Lin, C.-C., Gelatin-chondroitin-hyaluronan tri-copolymer scaffold for cartilage tissue engineering (2003) Biomaterials., 24, pp. 4853-4858; Concaro, S., Nicklasson, E., Ellowsson, L., Effect of cell seeding concentration on the quality of tissue engineered constructs loaded with adult human articular chondrocytes (2008) J Tissue Eng Regen Med., 2, pp. 14-21; Fan, H.-B., Hu, Y.-Y., Zhang, C.-L., Cartilage regeneration using mesenchymal stem cells and a PLGA-gelatin/chondroitin/hyaluronate hybrid scaffold (2006) Biomaterials., 27, pp. 4573-4580; Kim, H.J., Lee, J.H., Gun, G.I., Chondrogenesis using mesenchymal stem cells and PCL scaffolds (2010) J Biomed Mater Res A., 92 A, pp. 659-666; Izquierdo, R., Garcia-Giralt, N., Rodriguez, M.T., Biodegradable PCL scaffolds with an interconnected spherical pore network for tissue engineering (2008) J Biomed Mater Res A., 85 A, pp. 25-35; Guilak, F., Estes, B., Diekman, B., 2010 Nicolas Andry Award: Multipotent adult stem cells from adipose tissue for musculoskeletal tissue engineering (2010) Clin Orthop Relat Res., 468, pp. 2530-2540; Lee, R.H., Kim, B., Choi, I., Characterization and expression analysis of mesenchymal stem cells from human bone marrow and adipose tissue (2004) Cell Physiol Biochem., 14, pp. 311-324; Wagner, W., Wein, F., Seckinger, A., Comparative characteristics of mes-enchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood (2005) Exp Hematol., 33, pp. 1402-1416; Dicker, A., Le Blanc, K., Åström, G., Functional studies of mesenchymal stem cells derived from adult human adipose tissue (2005) Exp Cell Res., 308, pp. 283-290; Zuk, P.A., Zhu, M., Mizuno, H., Multilineage cells from human adipose tissue: Implications for cell-based therapies (2001) Tissue Eng., 7, pp. 211-228; Quintana, L., Zur Nieden, N.I., Semino, C.E., Morphogenetic and regulatory mechanisms during developmental chondrogenesis: New paradigms for cartilage tissue engineering (2009) Tissue Eng Part B Rev., 15, pp. 29-41; Chen, A.C., Lee, M.S., Lin, S.S., Augmentation of osteochondral repair with hyperbaric oxygenation: A rabbit study (2010) J Orthop Surg Res, 5, p. 91; Nilsson, P., Albrektsson, T., Granstrom, G., The effect of hyperbaric oxygen treatment on bone regeneration: An experimental study using the bone harvest chamber in the rabbit (1998) Int J Oral Maxillofac Implants., 3, pp. 43-48; Roberts, G.P., Harding, K.G., Stimulation of glycosaminoglycan synthesis in cultured fibroblasts by hyperbaric oxygen (1994) Br J Dermatol., 131, pp. 630-633; Kang, T.S., Gorti, G.K., Quan, S.Y., Effect of hyperbaric oxygen on the growth factor profile of fibroblasts (2004) Arch Facial Plast Surg., 6, pp. 31-35; Natesan, S., Baer, D.G., Walters, T.J., Adipose-derived stem cell delivery into collagen gels using chitosan microspheres (2010) Tissue Eng Part A., 16, pp. 1369-1384; Rigotti, G., Marchi, A., Galiè, M., Clinical treatment of radiotherapy tissue damage by lipoaspirate transplant: A healing process mediated by adipose-derived adult stem cells (2007) Plast Reconstr Surg., 119, pp. 1409-1422; Heng, B.C., Cao, T., Lee, E.H., Directing stem cell differentiation into the chon-drogenic lineage in vitro (2004) Stem Cells., 22, pp. 1152-1167; Hsieh, C.P., Chiou, Y.L., Lin, C.Y., Hyperbaric oxygen-stimulated proliferation and growth of osteoblasts may be mediated through the FGF-2/MEK/ERK 1/2/NF-kappaB and PKC/JNK pathways (2010) Connect Tissue Res., 51, pp. 497-509; Ogawa, R., Mizuno, S., Murphy, G.F., The effect of hydrostatic pressure on three-dimensional chondroinduction of human adipose-derived stem cells (2009) Tissue Eng Part A., 15, pp. 2937-2945

PY - 2012

Y1 - 2012

N2 - There is an urgent need to develop tissue-engineered cartilage for patients experiencing joint malfunction due to insufficient self-repairing capacity of articular cartilage. The aim of this research was to explore the effect of hyperbaric oxygen and air on tissue-engineered cartilage formation from human adipose-derived stem cells seeding on the gelatin/polycaprolactone biocomposites. The results of histological analyses indicate that under hyperbaric oxygen and air stimulation, the cell number of chondrocytes in cartilage matrix was not significantly increased, but the 1,9-dimethylmethylene blue assay showed that the glycosaminoglycans syntheses markedly increased compared to the control group. In quantification real-time polymerase chain reaction results, the chondrogenic-specific gene expression of SOX9, aggrecan, and COL2A1 were compared respectively. Within the limitation of this study, it was concluded that 2.5 atmosphere absolute oxygen and air may provide a stress environment to help cartilage tissue engineering development. Copyright © 2012 by Lippincott Williams & Wilkins.

AB - There is an urgent need to develop tissue-engineered cartilage for patients experiencing joint malfunction due to insufficient self-repairing capacity of articular cartilage. The aim of this research was to explore the effect of hyperbaric oxygen and air on tissue-engineered cartilage formation from human adipose-derived stem cells seeding on the gelatin/polycaprolactone biocomposites. The results of histological analyses indicate that under hyperbaric oxygen and air stimulation, the cell number of chondrocytes in cartilage matrix was not significantly increased, but the 1,9-dimethylmethylene blue assay showed that the glycosaminoglycans syntheses markedly increased compared to the control group. In quantification real-time polymerase chain reaction results, the chondrogenic-specific gene expression of SOX9, aggrecan, and COL2A1 were compared respectively. Within the limitation of this study, it was concluded that 2.5 atmosphere absolute oxygen and air may provide a stress environment to help cartilage tissue engineering development. Copyright © 2012 by Lippincott Williams & Wilkins.

KW - atmosphere absolute (ATA)

KW - cartilage tissue engineering

KW - gelatin/polycaprolactone

KW - glycosaminoglycan

KW - human adipose-derived stem cells (hASCs)

KW - hyperbaric oxygen

KW - adipocyte

KW - air

KW - analysis of variance

KW - article

KW - cartilage

KW - cell differentiation

KW - cell survival

KW - chondrogenesis

KW - cytology

KW - extracellular matrix

KW - genetics

KW - human

KW - methodology

KW - stem cell

KW - tissue engineering

KW - Adipocytes

KW - Air

KW - Analysis of Variance

KW - Cartilage

KW - Cell Differentiation

KW - Cell Survival

KW - Chondrogenesis

KW - Extracellular Matrix

KW - Humans

KW - Hyperbaric Oxygenation

KW - Stem Cells

KW - Tissue Engineering

U2 - 10.1097/SAP.0b013e3182745f95

DO - 10.1097/SAP.0b013e3182745f95

M3 - Article

VL - 69

SP - 650

EP - 655

JO - Annals of Plastic Surgery

JF - Annals of Plastic Surgery

SN - 0148-7043

IS - 6

ER -