The effect of human platelet lysate on the differentiation ability of human adipose-derived stem cells cultured on ECM-coated surfaces

Yan Gao, Nien Ju Ku, Tzu Cheng Sung, Akon Higuchi, Chi Sheng Hung, Henry Hsin Chung Lee, Qing Dong Ling, Nai Chen Cheng, Akihiro Umezawa, Lassina Barro, Thierry Burnouf, Qingsong Ye, Hao Chen

Research output: Contribution to journalArticle

Abstract

Human mesenchymal stem cells (hMSCs), such as human adipose-derived stem cells (hADSCs), present heterogeneous characteristics, including varying differentiation abilities and genotypes. hADSCs isolated under different conditions exhibit differences in stemness. We isolated hADSCs from human fat tissues via culture on different cell culture biomaterials including tissue culture polystyrene (TCPS) dishes and extracellular matrix protein (ECM)-coated dishes in medium supplemented with 5% or 10% serum-converted human platelet lysate (hPL) or 10% fetal bovine serum (FBS) as a control. Currently, it is not clear whether xeno-free hPL in the cell culture medium promotes the ability of hMSCs such as hADSCs to differentiate into several cell lineages compared to the xenomaterial FBS. We investigated whether a synchronized effect of ECM (Matrigel, fibronectin, and recombinant vitronectin) coatings on TCPS dishes for efficient hADSC differentiation could be observed when hADSCs were cultured in hPL medium. We found that Matrigel-coated dishes promoted hADSC differentiation into osteoblasts and suppressed differentiation into chondrocytes in 10% hPL medium. Recombinant vitronectin- A nd fibronectin-coated dishes greatly promoted hADSC differentiation into osteoblasts and chondrocytes in 5% and 10% hPL media. hPL promoted hADSC differentiation into osteoblasts and chondrocytes compared to FBS on the fibronectin-coated surface and recombinant vitronectin-coated surface.

Original languageEnglish
Pages (from-to)7110-7119
Number of pages10
JournalJournal of Materials Chemistry B
Volume7
Issue number45
DOIs
Publication statusPublished - Jan 1 2019

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Extracellular Matrix Proteins
Platelets
Stem cells
Proteins
Vitronectin
Osteoblasts
Fibronectins
Cell culture
Tissue culture
Polystyrenes
Biocompatible Materials
Oils and fats
Biomaterials
Culture Media
Fats
Tissue
Coatings

ASJC Scopus subject areas

  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)

Cite this

The effect of human platelet lysate on the differentiation ability of human adipose-derived stem cells cultured on ECM-coated surfaces. / Gao, Yan; Ku, Nien Ju; Sung, Tzu Cheng; Higuchi, Akon; Hung, Chi Sheng; Lee, Henry Hsin Chung; Ling, Qing Dong; Cheng, Nai Chen; Umezawa, Akihiro; Barro, Lassina; Burnouf, Thierry; Ye, Qingsong; Chen, Hao.

In: Journal of Materials Chemistry B, Vol. 7, No. 45, 01.01.2019, p. 7110-7119.

Research output: Contribution to journalArticle

Gao, Y, Ku, NJ, Sung, TC, Higuchi, A, Hung, CS, Lee, HHC, Ling, QD, Cheng, NC, Umezawa, A, Barro, L, Burnouf, T, Ye, Q & Chen, H 2019, 'The effect of human platelet lysate on the differentiation ability of human adipose-derived stem cells cultured on ECM-coated surfaces', Journal of Materials Chemistry B, vol. 7, no. 45, pp. 7110-7119. https://doi.org/10.1039/c9tb01764j
Gao, Yan ; Ku, Nien Ju ; Sung, Tzu Cheng ; Higuchi, Akon ; Hung, Chi Sheng ; Lee, Henry Hsin Chung ; Ling, Qing Dong ; Cheng, Nai Chen ; Umezawa, Akihiro ; Barro, Lassina ; Burnouf, Thierry ; Ye, Qingsong ; Chen, Hao. / The effect of human platelet lysate on the differentiation ability of human adipose-derived stem cells cultured on ECM-coated surfaces. In: Journal of Materials Chemistry B. 2019 ; Vol. 7, No. 45. pp. 7110-7119.
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