The effect of Gu-Sui-Bu (Drynaria fortunei J. Sm) on bone cell activities

Jui Sheng Sun, Chun Yu Lin, Guo Chung Dong, Shiow Yunn Sheu, Feng Huei Lin, Li Ting Chen, Yng Jiin Wang

Research output: Contribution to journalArticle

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Abstract

In the traditional Chinese medicine, Gu-Sui-Bu [Drynaria fortunei (kunze) J. Sm] has been reported as a good enhancer for bone healing. In this experiment, we investigate the biochemical effects of this traditional Chinese medicine on the bone cells culture. Different concentrations of crude extract of Gu-Sui-Bu were added to rat bone cells culture. The mitochondria activity of the bone cells after exposure was determined by colorimetric assay. Biochemical markers such as alkaline phosphatase (ALP), acid phosphatase (ACP) titer, prostaglandin E2 (PGE2) titer and the expression of both osteopontin and osteonectin mRNA were evaluated. The effect on the osteoclasts differentiation was evaluated by tartrate-resistant acid phosphatase (TRAP) stain. The most effective concentration of Gu-Sui-Bu on bone cells was 1 mg/ml. The addition of 1 mg/ml Gu-Sui-Bu to bone cells culture for 7 days can statistically increase the intracellular ALP amount; while the ACP and PGE2 amount in culture medium were significantly increased. In Northern blot analysis, the expression of both osteopontin and osteonectin mRNA were down-regulated after adding Gu-Sui-Bu into bone cells culture. The formation of multi-nucleated osteoclasts was more active than that of the control group; but no giant osteoclasts formation was observed. In this study, we demonstrated that Gu-Sui-Bu has potential effects on the bone cells culture. One of the major effects of Gu-Sui-Bu on the bone cells is probably mediated by its effect on the osteclasts activities. Continued and advanced study on the alterations in gene expression of bone cells by Chinese medicines will provide a basis for understanding the observed bone cell responses to various pharmacological interventions.

Original languageEnglish
Pages (from-to)3377-3385
Number of pages9
JournalBiomaterials
Volume23
Issue number16
DOIs
Publication statusPublished - 2002

Fingerprint

Polypodiaceae
Bone
Bone and Bones
Phosphatases
Cell culture
Cell Culture Techniques
Osteoclasts
Acid Phosphatase
Osteonectin
Medicine
Osteopontin
Chinese Traditional Medicine
Dinoprostone
Alkaline Phosphatase
Acids
gu-sui-bu
Messenger RNA
Mitochondria
Complex Mixtures
Gene expression

Keywords

  • Gu-Sui-Bu
  • In vitro
  • Osteoblast
  • Osteoclast
  • Osteopontin
  • Steonectin

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Sun, J. S., Lin, C. Y., Dong, G. C., Sheu, S. Y., Lin, F. H., Chen, L. T., & Wang, Y. J. (2002). The effect of Gu-Sui-Bu (Drynaria fortunei J. Sm) on bone cell activities. Biomaterials, 23(16), 3377-3385. https://doi.org/10.1016/S0142-9612(02)00038-8

The effect of Gu-Sui-Bu (Drynaria fortunei J. Sm) on bone cell activities. / Sun, Jui Sheng; Lin, Chun Yu; Dong, Guo Chung; Sheu, Shiow Yunn; Lin, Feng Huei; Chen, Li Ting; Wang, Yng Jiin.

In: Biomaterials, Vol. 23, No. 16, 2002, p. 3377-3385.

Research output: Contribution to journalArticle

Sun, JS, Lin, CY, Dong, GC, Sheu, SY, Lin, FH, Chen, LT & Wang, YJ 2002, 'The effect of Gu-Sui-Bu (Drynaria fortunei J. Sm) on bone cell activities', Biomaterials, vol. 23, no. 16, pp. 3377-3385. https://doi.org/10.1016/S0142-9612(02)00038-8
Sun, Jui Sheng ; Lin, Chun Yu ; Dong, Guo Chung ; Sheu, Shiow Yunn ; Lin, Feng Huei ; Chen, Li Ting ; Wang, Yng Jiin. / The effect of Gu-Sui-Bu (Drynaria fortunei J. Sm) on bone cell activities. In: Biomaterials. 2002 ; Vol. 23, No. 16. pp. 3377-3385.
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