The effect of Gu-Sui-Bu (Drynaria fortunei J. Sm) immobilized modified calcium hydrogenphosphate on bone cell activities

Jui Sheng Sun, Guo Chung Dong, Chun Yu Lin, Shiow Yunn Sheu, Feng Huei Lin, Li Ting Chen, Walter Hong Shong Chang, Yng Jiin Wang

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In our previous study, we have validated the efficacy and the safety of Gu-Sui-Bu [Drynaria fortunei (Kunze) J. Sm.] by the bone cells culture. However, a satisfactory delivery system for Gu-Sui-Bu must be developed before it can be used in clinical medicine. In this study, we try to use modified calcium hydrogenphosphate (MCHP) bioceramic as a carrier to transport Gu-Sui-Bu into the bone cell culture system. Toward this goal, we evaluated the effect of a Gu-Sui-Bu-immobilized modified calcium hydrogenphosphate (GI-MCHP) on the bone cells activities. The Chinese medicine: Gu-Sui-Bu [Drynaria fortunei (kunze) J. Sm] was extracted and then immobilized on the surface of MCHP. The rat osteoblasts-osteoclasts co-culture system was used as the experimental model. After the cells grew to 80% confluence, different sizes of GI-MCHP particles were tested. The mitochondria activity of the bone cells after exposure was determined by colorimetric assay. Biochemical markers such as lactate dehydrogenase (LDH), alkaline phosphatase (ALP), acid phosphatase (ACP) and prostaglandin E2 titer were analyzed to evaluate the bone cells activities. Histomorphometric study of osteoclasts activities and the phenotype expression of osteoblasts were also evaluated. There is no detectable titer of LDH secretion into the medium and no significant change in the intracellular ALP content. The ALP titer in the culture medium did increase significantly at 3 days' culture, while there is a significant decrease in the intracellular ACP content and significant increase in the ACP titer in the medium. The concentrations of PGE2 in tested medium are always significantly higher than that of control medium during the 7 days' culture. At the end of 7 days' culture, the PGE2 concentrations in the tested medium were still 4.74 times that of the control medium. After GI-MCHP treatment on bone cells, the size of the osteoclasts seems decreased and their cell integrity seems lost, while the osteoblasts phenotype expression was relatively preserved. From this study, we demonstrated that Gu-Sui-Bu [Drynaria fortunei (Kunze) J. Sm.] immobilized MCHP has well preserved the potential beneficial effects of Gu-Sui-Bu on the bone cells culture.

Original languageEnglish
Pages (from-to)873-882
Number of pages10
JournalBiomaterials
Volume24
Issue number5
DOIs
Publication statusPublished - Feb 2003

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Polypodiaceae
Phosphatases
Calcium
Bone
Bone and Bones
Cell culture
Osteoblasts
Osteoclasts
Acid Phosphatase
Dinoprostone
Alkaline Phosphatase
Medicine
Cell Culture Techniques
Acids
L-Lactate Dehydrogenase
Bioceramics
Mitochondria
Phenotype
gu-sui-bu
Clinical Medicine

Keywords

  • Bone cells
  • Calcium hydrogenphosphate
  • Gu-sui-bu
  • Prostaglandin
  • RTPCR

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

The effect of Gu-Sui-Bu (Drynaria fortunei J. Sm) immobilized modified calcium hydrogenphosphate on bone cell activities. / Sun, Jui Sheng; Dong, Guo Chung; Lin, Chun Yu; Sheu, Shiow Yunn; Lin, Feng Huei; Chen, Li Ting; Chang, Walter Hong Shong; Wang, Yng Jiin.

In: Biomaterials, Vol. 24, No. 5, 02.2003, p. 873-882.

Research output: Contribution to journalArticle

Sun, Jui Sheng ; Dong, Guo Chung ; Lin, Chun Yu ; Sheu, Shiow Yunn ; Lin, Feng Huei ; Chen, Li Ting ; Chang, Walter Hong Shong ; Wang, Yng Jiin. / The effect of Gu-Sui-Bu (Drynaria fortunei J. Sm) immobilized modified calcium hydrogenphosphate on bone cell activities. In: Biomaterials. 2003 ; Vol. 24, No. 5. pp. 873-882.
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