The effect of gelatin-chondroitin sulfate-hyaluronic acid skin substitute on wound healing in SCID mice

Tzu Wei Wang, Jui Sheng Sun, Hsi Chin Wu, Yang Hwei Tsuang, Wen Hsi Wang, Feng Huei Lin

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Tissue-engineered skin substitutes provided a feasibility to overcome the shortage of skin autograft by culturing keratinocytes and dermal fibroblasts in vitro. In this study, we applied bi-layer gelatin-chondrointin-6-sulfate-hyaluronic acid (gelatin-C6S-HA) biomatrices onto the severe combined immunodeficiency (SCID) mice to evaluate its effect on promoting wound healing. Human foreskin keratinocytes and dermal fibroblasts were cultured with reconstructed skin equivalent (rSE) for 7 days. The rSE was then grafted to the dorsum of SCID mice to evaluate its biocompatibility by histologic and immunohistochemistry analysis. The results showed that human epidermis were well-developed with the expression of differentiated markers and basement membrane-specific proteins at 4 weeks. After implantation, the percentages of skin graft take were satisfactory, while cell-seeded group was better than non-cell-seeded one. The basement membrane proteins including laminin, type IV collagen, type VII collagen, integrin α6, and integrin β4 were all detected at the dermal-epidermal junction, which showed a continuous structure in the 4 weeks after grafting. This bi-layer gelatin-C6S-HA skin substitute not only has positive effect on promoting wound healing, but also has high rate of graft take. This rSE would have the potential to be applied on the extensively and deeply burned patients who suffer from severe skin defect in the near future.

Original languageEnglish
Pages (from-to)5689-5697
Number of pages9
JournalBiomaterials
Volume27
Issue number33
DOIs
Publication statusPublished - Nov 2006
Externally publishedYes

Fingerprint

Artificial Skin
Hyaluronic acid
Severe Combined Immunodeficiency
Chondroitin Sulfates
Hyaluronic Acid
Gelatin
Wound Healing
Skin
Fibroblasts
Grafts
Integrins
Sulfates
Collagen Type VII
Collagen
Keratinocytes
Basement Membrane
Autografts
Membrane Proteins
Laminin
Proteins

Keywords

  • Biomimetic material
  • Transplantation
  • Wound healing

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

The effect of gelatin-chondroitin sulfate-hyaluronic acid skin substitute on wound healing in SCID mice. / Wang, Tzu Wei; Sun, Jui Sheng; Wu, Hsi Chin; Tsuang, Yang Hwei; Wang, Wen Hsi; Lin, Feng Huei.

In: Biomaterials, Vol. 27, No. 33, 11.2006, p. 5689-5697.

Research output: Contribution to journalArticle

Wang, Tzu Wei ; Sun, Jui Sheng ; Wu, Hsi Chin ; Tsuang, Yang Hwei ; Wang, Wen Hsi ; Lin, Feng Huei. / The effect of gelatin-chondroitin sulfate-hyaluronic acid skin substitute on wound healing in SCID mice. In: Biomaterials. 2006 ; Vol. 27, No. 33. pp. 5689-5697.
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