The down-regulation of galectin-1 expression is a specific biomarker of arsenic toxicity

Yu Ying Chang, Ming Chang Chiang, Tai Chih Kuo, Li Ling Chi, Yung Hsi Kao, Rong Nan Huang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Galectin-1 (GAL1) is known as a β-galactoside-binding protein that also can bind with arsenic to regulate cell functions. Using RNA interference technique, we investigated the possible mechanism involved in GAL1 modulation of arsenite-inhibited cell survival in 3T3 fibroblast and KB oral cancer cells. GAL1 gene knockdown significantly attenuated sodium arsenite (NaAsO2) and arsenic trioxide (As2O3) inhibition of cell survival. However, GAL1 gene knockdown did not alter the inhibition of cell survival by antimony chloride, cadmium chloride or nickel sulfate. These results suggested the GAL1 selectively affects particular types of heavy metal elements. Flow cytometric analysis indicated GAL1 gene knockdown also suppressed As(III)-stimulated levels of sub-G1 and G2/M growth arrest in both cells. Moreover, atomic absorption spectrophotometric results showed that GAL1 gene knockdown reduced the total arsenic accumulation of both cells after the NaAsO2 and As2O3 treatment. These results suggested that GAL1 gene knockdown mediates the apoptotic effects of arsenic in 3T3 and KB cells via regulation of the cellular arsenic levels. We propose that down-regulation of GAL1 expression may be a useful and specific biomarker in assessing the toxicity of arsenic exposure.

Original languageEnglish
Pages (from-to)38-46
Number of pages9
JournalToxicology Letters
Volume205
Issue number1
DOIs
Publication statusPublished - Aug 10 2011

Fingerprint

Galectin 1
Arsenic
Biomarkers
Toxicity
Down-Regulation
Gene Knockdown Techniques
Genes
Cells
Cell Survival
KB Cells
Antimony
Cadmium Chloride
Galactosides
3T3 Cells
Mouth Neoplasms
Fibroblasts
Heavy Metals
RNA Interference
Chlorides
Carrier Proteins

Keywords

  • Antimony
  • Arsenic
  • Cadmium
  • Galectin-1
  • Nickel

ASJC Scopus subject areas

  • Toxicology

Cite this

The down-regulation of galectin-1 expression is a specific biomarker of arsenic toxicity. / Chang, Yu Ying; Chiang, Ming Chang; Kuo, Tai Chih; Chi, Li Ling; Kao, Yung Hsi; Huang, Rong Nan.

In: Toxicology Letters, Vol. 205, No. 1, 10.08.2011, p. 38-46.

Research output: Contribution to journalArticle

Chang, Yu Ying ; Chiang, Ming Chang ; Kuo, Tai Chih ; Chi, Li Ling ; Kao, Yung Hsi ; Huang, Rong Nan. / The down-regulation of galectin-1 expression is a specific biomarker of arsenic toxicity. In: Toxicology Letters. 2011 ; Vol. 205, No. 1. pp. 38-46.
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