The diversity of lactic acid bacteria in a traditional Taiwanese millet alcoholic beverage during fermentation

Shiou Huei Chao, Hui Yu Huang, Ya Huei Kang, Koichi Watanabe, Ying Chieh Tsai

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A millet alcoholic beverage that is made primarily from glutinous rice or millet is a traditional drink of indigenous Taiwanese tribes. In this study, we used both culture-dependent and culture-independent methods to analyse the diversity of the lactic acid bacteria (LAB) that are present during the process of millet alcohol fermentation, including during starter preparation and brewing. Based on random amplified polymorphic DNA (RAPD)-PCR fingerprinting profiles, 147 LAB isolates were identified and categorised into 38 groups. Phylogenetic analyses of 16S rRNA, phenylalanyl-tRNA synthase alpha subunit (pheS) and RNA polymerase alpha subunit (rpoA) gene sequences identified representative strains from each of the 38 groups as belonging to the following ten species: Enterococcus faecium group, Lactobacillus brevis, Lactococcus garvieae, Lactococcus lactis, Leuconostoc carnosum, Leuconostoc (Leu.) mesenteroides, Pediococcus pentosaceus, Pediococcus stilesii, Weissella cibaria and Weissella soli. The results of the LAB species diversity studies were confirmed using 16S rRNA gene-based denaturing gradient gel electrophoresis (PCR-DGGE), which showed that P. pentosaceus was mainly involved in the fermentation in both the starter preparation and at the brewing stage. However, L. brevis, Leu. mesenteroides and P. stilesii were the predominant LAB strains found during the brewing stage.

Original languageEnglish
Pages (from-to)135-142
Number of pages8
JournalLWT - Food Science and Technology
Volume51
Issue number1
DOIs
Publication statusPublished - Apr 1 2013
Externally publishedYes

Fingerprint

Alcoholic Beverages
alcoholic beverages
millets
Fermentation
lactic acid bacteria
Lactic Acid
brewing
Weissella
Lactobacillus brevis
fermentation
Bacteria
Pediococcus pentosaceus
Leuconostoc mesenteroides
Leuconostoc carnosum
Weissella cibaria
ribosomal RNA
Lactococcus
Lactococcus garvieae
Pediococcus
Leuconostoc

Keywords

  • 16S rRNA
  • Lactic acid bacteria
  • Millet alcoholic beverage
  • PCR-DGGE

ASJC Scopus subject areas

  • Food Science

Cite this

The diversity of lactic acid bacteria in a traditional Taiwanese millet alcoholic beverage during fermentation. / Chao, Shiou Huei; Huang, Hui Yu; Kang, Ya Huei; Watanabe, Koichi; Tsai, Ying Chieh.

In: LWT - Food Science and Technology, Vol. 51, No. 1, 01.04.2013, p. 135-142.

Research output: Contribution to journalArticle

Chao, Shiou Huei ; Huang, Hui Yu ; Kang, Ya Huei ; Watanabe, Koichi ; Tsai, Ying Chieh. / The diversity of lactic acid bacteria in a traditional Taiwanese millet alcoholic beverage during fermentation. In: LWT - Food Science and Technology. 2013 ; Vol. 51, No. 1. pp. 135-142.
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