The cross-talk between transforming growth factor-beta1 and ultrasound stimulation during mechanotransduction of rat tenocytes

Yuan Hung Chao, Yang Hwei Tsuang, Jui Sheng Sun, Cheng Kung Cheng, Ming Hong Chen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Ultrasound is an effective noninvasive treatment for various tendinopathies. However, how tenocytes convert ultrasound stimulation into cascades of cellular and molecular events is not well understood. The purpose of this study is to elucidate the signaling pathways of tenocytes during ultrasound stimulation. Primary cultures of tenocytes were harvested from Achilles tendons of SpragueDawley rats. The viability and proliferation of tenocytes, their genes expression, and the signaling pathways after ultrasound treatment with or without specific inhibitors were evaluated and analyzed. The results showed that ultrasound treatment (100 mW/cm 2 for 20 min) significantly enhanced matrix metalloproteinase 13 (MMP-13), c-Fos, and c-Jun gene expression, increased JNK and p38, but not extracellular signal-regulated kinase-1/2 (ERK1/2), phosphorylation at 5 min, and sustained up to 60 min. JNK inhibitor and p38 inhibitor, but not ERK1/2 inhibitor, attenuated ultrasound-dependent induction of MMP-13 expression, indicating that the JNK and p38 pathways are required for ultrasound-induced MMP-13 expression in tenocytes. We also found that SB431542 (transforming growth factor-beta (TGF-β) receptor kinases inhibitor) suppressed ultrasound-induced MMP13 and c-Fos gene expression, and p38 phosphorylation. This study revealed that ultrasound treatment stimulates tenocytes proliferation and regulates their matrix metabolism through the cross-talk between TGF-β and ultrasound-induced mitogen-activated protein kinases (MAPKs) signaling pathways.

Original languageEnglish
Pages (from-to)313-321
Number of pages9
JournalConnective Tissue Research
Volume52
Issue number4
DOIs
Publication statusPublished - Aug 2011

Fingerprint

Transforming Growth Factor beta1
Rats
Ultrasonics
Matrix Metalloproteinase 13
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Gene Expression
Gene expression
Phosphorylation
jun Genes
fos Genes
Transforming Growth Factor beta Receptors
Tendinopathy
Achilles Tendon
MAP Kinase Signaling System
Mitogen-Activated Protein Kinases
Transforming Growth Factor beta
Tenocytes
Phosphotransferases
Tendons

Keywords

  • Gene expression
  • Mechanotransduction
  • Tenocytes
  • TGF-β1
  • Ultrasound

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Orthopedics and Sports Medicine
  • Rheumatology

Cite this

The cross-talk between transforming growth factor-beta1 and ultrasound stimulation during mechanotransduction of rat tenocytes. / Chao, Yuan Hung; Tsuang, Yang Hwei; Sun, Jui Sheng; Cheng, Cheng Kung; Chen, Ming Hong.

In: Connective Tissue Research, Vol. 52, No. 4, 08.2011, p. 313-321.

Research output: Contribution to journalArticle

Chao, Yuan Hung ; Tsuang, Yang Hwei ; Sun, Jui Sheng ; Cheng, Cheng Kung ; Chen, Ming Hong. / The cross-talk between transforming growth factor-beta1 and ultrasound stimulation during mechanotransduction of rat tenocytes. In: Connective Tissue Research. 2011 ; Vol. 52, No. 4. pp. 313-321.
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