Abstract

Background: A mesoporous bioactive glass (MBG) with a highly specific surface area and well-ordered pores is a suitable material to carry antibiotics. Fibrin and mesenchymal stem cells are used in promoting tissue repair and osteogenesis, respectively. Purposes: Tobramycin-loaded mesoporous bioactive glass (TMBG), fibrin and mesenchymal stem cells are used in this study to promote osteogenesis and against bacterial contamination in vitro. Methods: MBG was synthesized in a two-step acid-catalyzed self-assembly process with hydrothermal treatment. Tobramycin was loaded with MBG and fibrin to make a TMBG fibrin complex. The cell proliferation, osteogenic differentiation, Live/Dead staining and antibiotics inhibition zone test were evaluated for TMBG fibrin complex. Results: The experimental results showed a well-ordered hexagonally structured bioactive glass was synthesized. The TMBG fibrin complex cultivated with rabbit bone marrow mesenchymal stem cells (RBMSCs) showed good biocompatibility and increased expression of collagen type I, OPN, OCN and RUNX2 in cultivation period. The inhibition zone of TMBG fibrin complex increased while the concentration of tobramycin increased against Staphylococcus aureus and Pseudomonas aeruginosa. In addition, the TMBG fibrin complex (with 0.5% tobramycin) had the same antibacterial effect as 10 mg tobramycin. Conclusions: Based on the results mentioned above, the TMBG fibrin complex showed antibacterial effect and exhibited great biocompatibility a potential biomaterial for bone tissue engineering.
Original languageEnglish
Pages (from-to)69-78
Number of pages10
JournalFormosan Journal of Musculoskeletal Disorders
Volume7
Issue number2
DOIs
Publication statusPublished - 2016

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Tobramycin
Bone Regeneration
Fibrin
Glass
Anti-Bacterial Agents
Mesenchymal Stromal Cells
Osteogenesis
Biocompatible Materials
Tissue Engineering
Collagen Type I
Pseudomonas aeruginosa
Staphylococcus aureus
Bone Marrow
Cell Proliferation
Staining and Labeling
Rabbits

Keywords

  • bone defects
  • drug delivery system
  • infection
  • mesoporous bioactive glass (MBG)
  • rabbit bone marrow mesenchymal stem cells
  • tobramycin

Cite this

The combination of mesoporous bioactive glass with fibrin and antibiotic for bone regeneration. / Chen, Lei-Yen; Chen, Wei-Chuan; Liu, Hsia-Wei; Lin, Hsiao-Mei; Liu, Hsien-Tao; Chen, Chih-Hwa.

In: Formosan Journal of Musculoskeletal Disorders, Vol. 7, No. 2, 2016, p. 69-78.

Research output: Contribution to journalArticle

Chen, Lei-Yen ; Chen, Wei-Chuan ; Liu, Hsia-Wei ; Lin, Hsiao-Mei ; Liu, Hsien-Tao ; Chen, Chih-Hwa. / The combination of mesoporous bioactive glass with fibrin and antibiotic for bone regeneration. In: Formosan Journal of Musculoskeletal Disorders. 2016 ; Vol. 7, No. 2. pp. 69-78.
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abstract = "Background: A mesoporous bioactive glass (MBG) with a highly specific surface area and well-ordered pores is a suitable material to carry antibiotics. Fibrin and mesenchymal stem cells are used in promoting tissue repair and osteogenesis, respectively. Purposes: Tobramycin-loaded mesoporous bioactive glass (TMBG), fibrin and mesenchymal stem cells are used in this study to promote osteogenesis and against bacterial contamination in vitro. Methods: MBG was synthesized in a two-step acid-catalyzed self-assembly process with hydrothermal treatment. Tobramycin was loaded with MBG and fibrin to make a TMBG fibrin complex. The cell proliferation, osteogenic differentiation, Live/Dead staining and antibiotics inhibition zone test were evaluated for TMBG fibrin complex. Results: The experimental results showed a well-ordered hexagonally structured bioactive glass was synthesized. The TMBG fibrin complex cultivated with rabbit bone marrow mesenchymal stem cells (RBMSCs) showed good biocompatibility and increased expression of collagen type I, OPN, OCN and RUNX2 in cultivation period. The inhibition zone of TMBG fibrin complex increased while the concentration of tobramycin increased against Staphylococcus aureus and Pseudomonas aeruginosa. In addition, the TMBG fibrin complex (with 0.5{\%} tobramycin) had the same antibacterial effect as 10 mg tobramycin. Conclusions: Based on the results mentioned above, the TMBG fibrin complex showed antibacterial effect and exhibited great biocompatibility a potential biomaterial for bone tissue engineering.",
keywords = "bone defects, drug delivery system, infection, mesoporous bioactive glass (MBG), rabbit bone marrow mesenchymal stem cells, tobramycin",
author = "Lei-Yen Chen and Wei-Chuan Chen and Hsia-Wei Liu and Hsiao-Mei Lin and Hsien-Tao Liu and Chih-Hwa Chen",
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T1 - The combination of mesoporous bioactive glass with fibrin and antibiotic for bone regeneration

AU - Chen, Lei-Yen

AU - Chen, Wei-Chuan

AU - Liu, Hsia-Wei

AU - Lin, Hsiao-Mei

AU - Liu, Hsien-Tao

AU - Chen, Chih-Hwa

PY - 2016

Y1 - 2016

N2 - Background: A mesoporous bioactive glass (MBG) with a highly specific surface area and well-ordered pores is a suitable material to carry antibiotics. Fibrin and mesenchymal stem cells are used in promoting tissue repair and osteogenesis, respectively. Purposes: Tobramycin-loaded mesoporous bioactive glass (TMBG), fibrin and mesenchymal stem cells are used in this study to promote osteogenesis and against bacterial contamination in vitro. Methods: MBG was synthesized in a two-step acid-catalyzed self-assembly process with hydrothermal treatment. Tobramycin was loaded with MBG and fibrin to make a TMBG fibrin complex. The cell proliferation, osteogenic differentiation, Live/Dead staining and antibiotics inhibition zone test were evaluated for TMBG fibrin complex. Results: The experimental results showed a well-ordered hexagonally structured bioactive glass was synthesized. The TMBG fibrin complex cultivated with rabbit bone marrow mesenchymal stem cells (RBMSCs) showed good biocompatibility and increased expression of collagen type I, OPN, OCN and RUNX2 in cultivation period. The inhibition zone of TMBG fibrin complex increased while the concentration of tobramycin increased against Staphylococcus aureus and Pseudomonas aeruginosa. In addition, the TMBG fibrin complex (with 0.5% tobramycin) had the same antibacterial effect as 10 mg tobramycin. Conclusions: Based on the results mentioned above, the TMBG fibrin complex showed antibacterial effect and exhibited great biocompatibility a potential biomaterial for bone tissue engineering.

AB - Background: A mesoporous bioactive glass (MBG) with a highly specific surface area and well-ordered pores is a suitable material to carry antibiotics. Fibrin and mesenchymal stem cells are used in promoting tissue repair and osteogenesis, respectively. Purposes: Tobramycin-loaded mesoporous bioactive glass (TMBG), fibrin and mesenchymal stem cells are used in this study to promote osteogenesis and against bacterial contamination in vitro. Methods: MBG was synthesized in a two-step acid-catalyzed self-assembly process with hydrothermal treatment. Tobramycin was loaded with MBG and fibrin to make a TMBG fibrin complex. The cell proliferation, osteogenic differentiation, Live/Dead staining and antibiotics inhibition zone test were evaluated for TMBG fibrin complex. Results: The experimental results showed a well-ordered hexagonally structured bioactive glass was synthesized. The TMBG fibrin complex cultivated with rabbit bone marrow mesenchymal stem cells (RBMSCs) showed good biocompatibility and increased expression of collagen type I, OPN, OCN and RUNX2 in cultivation period. The inhibition zone of TMBG fibrin complex increased while the concentration of tobramycin increased against Staphylococcus aureus and Pseudomonas aeruginosa. In addition, the TMBG fibrin complex (with 0.5% tobramycin) had the same antibacterial effect as 10 mg tobramycin. Conclusions: Based on the results mentioned above, the TMBG fibrin complex showed antibacterial effect and exhibited great biocompatibility a potential biomaterial for bone tissue engineering.

KW - bone defects

KW - drug delivery system

KW - infection

KW - mesoporous bioactive glass (MBG)

KW - rabbit bone marrow mesenchymal stem cells

KW - tobramycin

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DO - 10.6492/FJMD.20150531

M3 - Article

VL - 7

SP - 69

EP - 78

JO - Formosan Journal of Musculoskeletal Disorders

JF - Formosan Journal of Musculoskeletal Disorders

SN - 2210-7940

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ER -