The coexistence of hypertension and ovariectomy additively increases cardiac apoptosis

Yi Yuan Lin, Yu Jung Cheng, Jun Hu, Li Xi Chu, Woei Cherng Shyu, Chung Lan Kao, Tzer Bin Lin, Chia Hua Kuo, Ai Lun Yang, Shin Da Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

To investigate whether the coexistence of hypertension and ovariectomy will increase cardiac Fas receptor and mitochondrial-dependent apoptotic pathways, histopathological analysis, the TUNEL assay andWestern blotting were performed on the excised hearts from three groups of female spontaneously hypertensive rats (SHR), which were divided into a sham-operated group (SHR-Sham), bilaterally ovariectomized group (SHR-OVX) and normotensive Wistar Kyoto rats (WKY). Compared with the WKY group, the SHR-Sham group exhibited decreased protein levels of ER_, ER_, p-Akt/Akt, Bcl-2, Bcl-xL and p-Bad and decreased further in the SHR-OVX group, as well as protein levels of t-Bid, Bak, Bad, Bax, cytochrome c, activated caspase-9 and activated caspase-3 (mitochondria-dependent apoptosis) increased in the SHR-Sham group and increased further in the SHR-OVX group. Compared with the WKY group, protein levels of Fas ligand, TNF-_, Fas death receptors, TNFR1, FADD and activated caspase-8 (Fas receptor-dependent apoptosis) increased in the SHR-Sham group, but did not increase in the SHR-OVX group, except Fas ligand and TNF-_. The coexistence of hypertension and ovariectomy attenuated the estrogen receptor survival pathway and appeared to additively increase the cardiac mitochondria-dependent, but not the Fas receptor-dependent apoptosis pathway, which might provide one possible mechanism for the development of cardiac abnormalities in hypertensive postmenopausal women.

Original languageEnglish
Article number2036
JournalInternational Journal of Molecular Sciences
Volume17
Issue number12
DOIs
Publication statusPublished - Dec 6 2016

Keywords

  • Caspase
  • Cell death
  • Heart
  • Menopausal hypertension

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Spectroscopy
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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  • Cite this

    Lin, Y. Y., Cheng, Y. J., Hu, J., Chu, L. X., Shyu, W. C., Kao, C. L., Lin, T. B., Kuo, C. H., Yang, A. L., & Lee, S. D. (2016). The coexistence of hypertension and ovariectomy additively increases cardiac apoptosis. International Journal of Molecular Sciences, 17(12), [2036]. https://doi.org/10.3390/ijms17122036