Abstract
To investigate whether the coexistence of hypertension and ovariectomy will increase cardiac Fas receptor and mitochondrial-dependent apoptotic pathways, histopathological analysis, the TUNEL assay andWestern blotting were performed on the excised hearts from three groups of female spontaneously hypertensive rats (SHR), which were divided into a sham-operated group (SHR-Sham), bilaterally ovariectomized group (SHR-OVX) and normotensive Wistar Kyoto rats (WKY). Compared with the WKY group, the SHR-Sham group exhibited decreased protein levels of ER_, ER_, p-Akt/Akt, Bcl-2, Bcl-xL and p-Bad and decreased further in the SHR-OVX group, as well as protein levels of t-Bid, Bak, Bad, Bax, cytochrome c, activated caspase-9 and activated caspase-3 (mitochondria-dependent apoptosis) increased in the SHR-Sham group and increased further in the SHR-OVX group. Compared with the WKY group, protein levels of Fas ligand, TNF-_, Fas death receptors, TNFR1, FADD and activated caspase-8 (Fas receptor-dependent apoptosis) increased in the SHR-Sham group, but did not increase in the SHR-OVX group, except Fas ligand and TNF-_. The coexistence of hypertension and ovariectomy attenuated the estrogen receptor survival pathway and appeared to additively increase the cardiac mitochondria-dependent, but not the Fas receptor-dependent apoptosis pathway, which might provide one possible mechanism for the development of cardiac abnormalities in hypertensive postmenopausal women.
| Original language | English |
|---|---|
| Article number | 2036 |
| Journal | International Journal of Molecular Sciences |
| Volume | 17 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Dec 6 2016 |
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Keywords
- Caspase
- Cell death
- Heart
- Menopausal hypertension
ASJC Scopus subject areas
- Catalysis
- Molecular Biology
- Computer Science Applications
- Spectroscopy
- Physical and Theoretical Chemistry
- Organic Chemistry
- Inorganic Chemistry
Cite this
The coexistence of hypertension and ovariectomy additively increases cardiac apoptosis. / Lin, Yi Yuan; Cheng, Yu Jung; Hu, Jun; Chu, Li Xi; Shyu, Woei Cherng; Kao, Chung Lan; Lin, Tzer Bin; Kuo, Chia Hua; Yang, Ai Lun; Lee, Shin Da.
In: International Journal of Molecular Sciences, Vol. 17, No. 12, 2036, 06.12.2016.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The coexistence of hypertension and ovariectomy additively increases cardiac apoptosis
AU - Lin, Yi Yuan
AU - Cheng, Yu Jung
AU - Hu, Jun
AU - Chu, Li Xi
AU - Shyu, Woei Cherng
AU - Kao, Chung Lan
AU - Lin, Tzer Bin
AU - Kuo, Chia Hua
AU - Yang, Ai Lun
AU - Lee, Shin Da
PY - 2016/12/6
Y1 - 2016/12/6
N2 - To investigate whether the coexistence of hypertension and ovariectomy will increase cardiac Fas receptor and mitochondrial-dependent apoptotic pathways, histopathological analysis, the TUNEL assay andWestern blotting were performed on the excised hearts from three groups of female spontaneously hypertensive rats (SHR), which were divided into a sham-operated group (SHR-Sham), bilaterally ovariectomized group (SHR-OVX) and normotensive Wistar Kyoto rats (WKY). Compared with the WKY group, the SHR-Sham group exhibited decreased protein levels of ER_, ER_, p-Akt/Akt, Bcl-2, Bcl-xL and p-Bad and decreased further in the SHR-OVX group, as well as protein levels of t-Bid, Bak, Bad, Bax, cytochrome c, activated caspase-9 and activated caspase-3 (mitochondria-dependent apoptosis) increased in the SHR-Sham group and increased further in the SHR-OVX group. Compared with the WKY group, protein levels of Fas ligand, TNF-_, Fas death receptors, TNFR1, FADD and activated caspase-8 (Fas receptor-dependent apoptosis) increased in the SHR-Sham group, but did not increase in the SHR-OVX group, except Fas ligand and TNF-_. The coexistence of hypertension and ovariectomy attenuated the estrogen receptor survival pathway and appeared to additively increase the cardiac mitochondria-dependent, but not the Fas receptor-dependent apoptosis pathway, which might provide one possible mechanism for the development of cardiac abnormalities in hypertensive postmenopausal women.
AB - To investigate whether the coexistence of hypertension and ovariectomy will increase cardiac Fas receptor and mitochondrial-dependent apoptotic pathways, histopathological analysis, the TUNEL assay andWestern blotting were performed on the excised hearts from three groups of female spontaneously hypertensive rats (SHR), which were divided into a sham-operated group (SHR-Sham), bilaterally ovariectomized group (SHR-OVX) and normotensive Wistar Kyoto rats (WKY). Compared with the WKY group, the SHR-Sham group exhibited decreased protein levels of ER_, ER_, p-Akt/Akt, Bcl-2, Bcl-xL and p-Bad and decreased further in the SHR-OVX group, as well as protein levels of t-Bid, Bak, Bad, Bax, cytochrome c, activated caspase-9 and activated caspase-3 (mitochondria-dependent apoptosis) increased in the SHR-Sham group and increased further in the SHR-OVX group. Compared with the WKY group, protein levels of Fas ligand, TNF-_, Fas death receptors, TNFR1, FADD and activated caspase-8 (Fas receptor-dependent apoptosis) increased in the SHR-Sham group, but did not increase in the SHR-OVX group, except Fas ligand and TNF-_. The coexistence of hypertension and ovariectomy attenuated the estrogen receptor survival pathway and appeared to additively increase the cardiac mitochondria-dependent, but not the Fas receptor-dependent apoptosis pathway, which might provide one possible mechanism for the development of cardiac abnormalities in hypertensive postmenopausal women.
KW - Caspase
KW - Cell death
KW - Heart
KW - Menopausal hypertension
UR - http://www.scopus.com/inward/record.url?scp=85004091916&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85004091916&partnerID=8YFLogxK
U2 - 10.3390/ijms17122036
DO - 10.3390/ijms17122036
M3 - Article
C2 - 27929425
AN - SCOPUS:85004091916
VL - 17
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 12
M1 - 2036
ER -