The chloroplast genome of Phalaenopsis aphrodite (Orchidaceae)

Comparative analysis of evolutionary rate with that of grasses and its phylogenetic implications

Ching Chun Chang, Hsien Chia Lin, I. Pin Lin, Teh Yuan Chow, Hong Hwa Chen, Wen Huei Chen, Chia Hsiung Cheng, Chung Yen Lin, Shu Mei Liu, Chien Chang Chang, Shu Miaw Chaw

Research output: Contribution to journalArticle

182 Citations (Scopus)

Abstract

Whether the Amborella/Amborella-Nymphaeales or the grass lineage diverged first within the angiosperms has recently been debated. Central to this issue has been focused on the artifacts that might result from sampling only grasses within the monocots. We therefore sequenced the entire chloroplast genome (cpDNA) of Phalaenopsis aphrodite, Taiwan moth orchid. The cpDNA is a circular molecule of 148,964 bp with a comparatively short single-copy region (11,543 bp) due to the unusual loss and truncation/scattered deletion of certain ndh subunits. An open reading frame, orf91, located in the complementary strand of the rrn23 was reported for the first time. A comparison of nucleotide substitutions between P. aphrodite and the grasses indicates that only the plastid expression genes have a strong positive correlation between nonsynonymous (Ka) and synonymous (Ks) substitutions per site, providing evidence for a generation time effect, mainly across these genes. Among the intron-containing protein-coding genes of the sampled monocots, the Ks of the genes are significantly correlated to transitional substitutions of their introns. We compiled a concatenated 61 protein-coding gene alignment for the available 20 cpDNAs of vascular plants and analyzed the data set using Bayesian inference, maximum parsimony, and neighbor-joining (NJ) methods. The analyses yielded robust support for the Amborella/Amborella- Nymphaeales-basal hypothesis and for the orchid and grasses together being a monophyletic group nested within the remaining angiosperms. However, the NJ analysis using Ka, the first two codon positions, or amino acid sequences, respectively, supports the monocots-basal hypothesis. We demonstrated that these conflicting angiosperm phylogenies are most probably linked to the transitional sites at all codon positions, especially at the third one where the strong base-composition bias and saturation effect take place.

Original languageEnglish
Pages (from-to)279-291
Number of pages13
JournalMolecular Biology and Evolution
Volume23
Issue number2
DOIs
Publication statusPublished - Feb 2006
Externally publishedYes

Fingerprint

Amborella
Orchidaceae
Chloroplast Genome
Phalaenopsis
Poaceae
Angiosperms
chloroplast
genome
Genes
Liliopsida
Nymphaeales
angiosperm
grass
grasses
phylogenetics
Angiospermae
substitution
gene
phylogeny
chloroplast DNA

Keywords

  • Angiosperms
  • Chloroplast genome
  • Molecular evolution
  • Orchidaceae
  • Phalaenopsis aphrodite
  • Phylogeny
  • Poaceae
  • Substitution rate

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Genetics(clinical)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Ecology, Evolution, Behavior and Systematics
  • Agricultural and Biological Sciences (miscellaneous)
  • Molecular Biology

Cite this

The chloroplast genome of Phalaenopsis aphrodite (Orchidaceae) : Comparative analysis of evolutionary rate with that of grasses and its phylogenetic implications. / Chang, Ching Chun; Lin, Hsien Chia; Lin, I. Pin; Chow, Teh Yuan; Chen, Hong Hwa; Chen, Wen Huei; Cheng, Chia Hsiung; Lin, Chung Yen; Liu, Shu Mei; Chang, Chien Chang; Chaw, Shu Miaw.

In: Molecular Biology and Evolution, Vol. 23, No. 2, 02.2006, p. 279-291.

Research output: Contribution to journalArticle

Chang, Ching Chun ; Lin, Hsien Chia ; Lin, I. Pin ; Chow, Teh Yuan ; Chen, Hong Hwa ; Chen, Wen Huei ; Cheng, Chia Hsiung ; Lin, Chung Yen ; Liu, Shu Mei ; Chang, Chien Chang ; Chaw, Shu Miaw. / The chloroplast genome of Phalaenopsis aphrodite (Orchidaceae) : Comparative analysis of evolutionary rate with that of grasses and its phylogenetic implications. In: Molecular Biology and Evolution. 2006 ; Vol. 23, No. 2. pp. 279-291.
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