The cancer marker neutrophil gelatinase-associated lipocalin is highly expressed in human endometrial hyperplasia

Chi Jr Liao, Yen Hua Huang, Heng Kien Au, Le Ming Wang, Sin Tak Chu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Recently, endometrial hyperplasia was identified as presenting a higher risk for progressing to endometrial carcinoma more readily than adenomyosis. The Lcn-2 gene encodes neutrophil gelatinase-associated lipocalin (NGAL), which promotes cell proliferation and serves as a cancer markerinsome cancers. Inour current study, we investigated the relationship between the expression of NGAL and that of pathogenic cytokines and cancer-related genes including cyclooxygenase-2 (COX-2), E-cadherin, β-catenin, and vimentin in patients with endometrial disorders. NGAL expression was examined by Western blotting, immunohis-tochemistry, and reverse-transcription polymerase chain reaction (RT-PCR) in hyperplasia and adenomyosis biopsy samples. Immunohistochemistry demonstrated the occurrence of NGAL in glandular epithelial cells but not in the stromal cells of hyperplasia biopsy samples. NGAL protein and mRNA expression were significantly greater in endo-metrial hyperplasia than in endometrial adenomyosis. Although our data showed no difference in pathogenic cytokines between patients with endometrial hyperplasia and endometrial adenomyosis, we observed high expression levels of COX-2, β-catenin, vimentin, and E-cadherin in patients with endometrial hyperplasia. NGAL mRNA expression correlated positively with COX-2 and E-cadherin mRNA expression (r = 0.41 and r = 0.57, respectively), but correlated negatively with vimentin and β-catenin mRNA expression (r = -0.42 and r = -0.61, respectively). Our data suggest that NGAL is up-regulated in patients with endometrial hyperplasia to prevent the transition from hyperplasia to carcinoma.

Original languageEnglish
Pages (from-to)1029-1036
Number of pages8
JournalMolecular Biology Reports
Volume39
Issue number2
DOIs
Publication statusPublished - Feb 2012

Keywords

  • Adenomyosis
  • Endometrial hyperplasia
  • Lipocalin 2

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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