The association of human connexin 40 genetic polymorphisms with atrial fibrillation

Jyh Ming Juang, Yi Rong Chern, Chia Ti Tsai, Fu Tien Chiang, Jiunn Lee Lin, Juey Jen Hwang, Kwan Li Hsu, Chuen Den Tseng, Yung Zu Tseng, Ling Ping Lai

Research output: Contribution to journalArticle

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Abstract

Background: There is evidence showing that genetic factors contribute to the pathogenesis of atrial fibrillation (Af). We investigated the association between Af and polymorphisms of the connexin 40 (Cx40) gene, which is important in the electrical coupling between atrial myocytes. Methods: We performed an association study between two Cx40 single nucleotide polymorphisms (SNPs) (Cx40 - 44 and + 71 allele) and Af. We enrolled 173 patients with Af, and the control group consisted of 232 patients without Af. The luciferase assay was performed to evaluate the promoter activities of different Cx40 haplotypes in cultured atrial myocytes. Results: We found that the two SNPs were both significantly associated with Af. In pairwise linkage disequilibrium analysis, the two SNPs were completely linked (Cx40 - 44G always associated with Cx40 + 71A; Cx40 - 44A associated with Cx40 + 71G, P < 0.001). In haplotype analysis, we demonstrated that the frequency of Cx40 (- 44A,+ 71G) was significantly higher in the Af group than that in the control group (P < 0.006, odds ratio = 1.514, 95% confidence interval 1.13-2.04). We also performed genotype analysis using several genetic models, finding that the recessive model showed the lowest P value (P < 0.004) and the largest odds ratio (2.53, 95% confidence interval 1.23-5.19). In promoter activity studies using luciferase as the reporter, Cx40 (- 44A,+ 71G) had significantly lower promoter activity than that of the Cx40 (- 44G,+ 71A) in atrial myocytes. Conclusions: The two SNPs in the promoter region of the Cx40 gene were significantly associated with Af. The Cx40 (- 44A + 71G) haplotype was associated with a higher risk for Af. This haplotype also had significantly lower promoter activity in atrial myocytes.

Original languageEnglish
Pages (from-to)107-112
Number of pages6
JournalInternational Journal of Cardiology
Volume116
Issue number1
DOIs
Publication statusPublished - Mar 2 2007
Externally publishedYes

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Genetic Polymorphisms
Atrial Fibrillation
Muscle Cells
Haplotypes
Single Nucleotide Polymorphism
Luciferases
connexin 40
Odds Ratio
Confidence Intervals
Control Groups
Genetic Models
Linkage Disequilibrium
Genetic Promoter Regions
Genes
Alleles
Genotype

Keywords

  • Atrial fibrillation
  • Connexin
  • Single nucleotide polymorphism

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

The association of human connexin 40 genetic polymorphisms with atrial fibrillation. / Juang, Jyh Ming; Chern, Yi Rong; Tsai, Chia Ti; Chiang, Fu Tien; Lin, Jiunn Lee; Hwang, Juey Jen; Hsu, Kwan Li; Tseng, Chuen Den; Tseng, Yung Zu; Lai, Ling Ping.

In: International Journal of Cardiology, Vol. 116, No. 1, 02.03.2007, p. 107-112.

Research output: Contribution to journalArticle

Juang, JM, Chern, YR, Tsai, CT, Chiang, FT, Lin, JL, Hwang, JJ, Hsu, KL, Tseng, CD, Tseng, YZ & Lai, LP 2007, 'The association of human connexin 40 genetic polymorphisms with atrial fibrillation', International Journal of Cardiology, vol. 116, no. 1, pp. 107-112. https://doi.org/10.1016/j.ijcard.2006.03.037
Juang, Jyh Ming ; Chern, Yi Rong ; Tsai, Chia Ti ; Chiang, Fu Tien ; Lin, Jiunn Lee ; Hwang, Juey Jen ; Hsu, Kwan Li ; Tseng, Chuen Den ; Tseng, Yung Zu ; Lai, Ling Ping. / The association of human connexin 40 genetic polymorphisms with atrial fibrillation. In: International Journal of Cardiology. 2007 ; Vol. 116, No. 1. pp. 107-112.
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abstract = "Background: There is evidence showing that genetic factors contribute to the pathogenesis of atrial fibrillation (Af). We investigated the association between Af and polymorphisms of the connexin 40 (Cx40) gene, which is important in the electrical coupling between atrial myocytes. Methods: We performed an association study between two Cx40 single nucleotide polymorphisms (SNPs) (Cx40 - 44 and + 71 allele) and Af. We enrolled 173 patients with Af, and the control group consisted of 232 patients without Af. The luciferase assay was performed to evaluate the promoter activities of different Cx40 haplotypes in cultured atrial myocytes. Results: We found that the two SNPs were both significantly associated with Af. In pairwise linkage disequilibrium analysis, the two SNPs were completely linked (Cx40 - 44G always associated with Cx40 + 71A; Cx40 - 44A associated with Cx40 + 71G, P < 0.001). In haplotype analysis, we demonstrated that the frequency of Cx40 (- 44A,+ 71G) was significantly higher in the Af group than that in the control group (P < 0.006, odds ratio = 1.514, 95{\%} confidence interval 1.13-2.04). We also performed genotype analysis using several genetic models, finding that the recessive model showed the lowest P value (P < 0.004) and the largest odds ratio (2.53, 95{\%} confidence interval 1.23-5.19). In promoter activity studies using luciferase as the reporter, Cx40 (- 44A,+ 71G) had significantly lower promoter activity than that of the Cx40 (- 44G,+ 71A) in atrial myocytes. Conclusions: The two SNPs in the promoter region of the Cx40 gene were significantly associated with Af. The Cx40 (- 44A + 71G) haplotype was associated with a higher risk for Af. This haplotype also had significantly lower promoter activity in atrial myocytes.",
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AU - Juang, Jyh Ming

AU - Chern, Yi Rong

AU - Tsai, Chia Ti

AU - Chiang, Fu Tien

AU - Lin, Jiunn Lee

AU - Hwang, Juey Jen

AU - Hsu, Kwan Li

AU - Tseng, Chuen Den

AU - Tseng, Yung Zu

AU - Lai, Ling Ping

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N2 - Background: There is evidence showing that genetic factors contribute to the pathogenesis of atrial fibrillation (Af). We investigated the association between Af and polymorphisms of the connexin 40 (Cx40) gene, which is important in the electrical coupling between atrial myocytes. Methods: We performed an association study between two Cx40 single nucleotide polymorphisms (SNPs) (Cx40 - 44 and + 71 allele) and Af. We enrolled 173 patients with Af, and the control group consisted of 232 patients without Af. The luciferase assay was performed to evaluate the promoter activities of different Cx40 haplotypes in cultured atrial myocytes. Results: We found that the two SNPs were both significantly associated with Af. In pairwise linkage disequilibrium analysis, the two SNPs were completely linked (Cx40 - 44G always associated with Cx40 + 71A; Cx40 - 44A associated with Cx40 + 71G, P < 0.001). In haplotype analysis, we demonstrated that the frequency of Cx40 (- 44A,+ 71G) was significantly higher in the Af group than that in the control group (P < 0.006, odds ratio = 1.514, 95% confidence interval 1.13-2.04). We also performed genotype analysis using several genetic models, finding that the recessive model showed the lowest P value (P < 0.004) and the largest odds ratio (2.53, 95% confidence interval 1.23-5.19). In promoter activity studies using luciferase as the reporter, Cx40 (- 44A,+ 71G) had significantly lower promoter activity than that of the Cx40 (- 44G,+ 71A) in atrial myocytes. Conclusions: The two SNPs in the promoter region of the Cx40 gene were significantly associated with Af. The Cx40 (- 44A + 71G) haplotype was associated with a higher risk for Af. This haplotype also had significantly lower promoter activity in atrial myocytes.

AB - Background: There is evidence showing that genetic factors contribute to the pathogenesis of atrial fibrillation (Af). We investigated the association between Af and polymorphisms of the connexin 40 (Cx40) gene, which is important in the electrical coupling between atrial myocytes. Methods: We performed an association study between two Cx40 single nucleotide polymorphisms (SNPs) (Cx40 - 44 and + 71 allele) and Af. We enrolled 173 patients with Af, and the control group consisted of 232 patients without Af. The luciferase assay was performed to evaluate the promoter activities of different Cx40 haplotypes in cultured atrial myocytes. Results: We found that the two SNPs were both significantly associated with Af. In pairwise linkage disequilibrium analysis, the two SNPs were completely linked (Cx40 - 44G always associated with Cx40 + 71A; Cx40 - 44A associated with Cx40 + 71G, P < 0.001). In haplotype analysis, we demonstrated that the frequency of Cx40 (- 44A,+ 71G) was significantly higher in the Af group than that in the control group (P < 0.006, odds ratio = 1.514, 95% confidence interval 1.13-2.04). We also performed genotype analysis using several genetic models, finding that the recessive model showed the lowest P value (P < 0.004) and the largest odds ratio (2.53, 95% confidence interval 1.23-5.19). In promoter activity studies using luciferase as the reporter, Cx40 (- 44A,+ 71G) had significantly lower promoter activity than that of the Cx40 (- 44G,+ 71A) in atrial myocytes. Conclusions: The two SNPs in the promoter region of the Cx40 gene were significantly associated with Af. The Cx40 (- 44A + 71G) haplotype was associated with a higher risk for Af. This haplotype also had significantly lower promoter activity in atrial myocytes.

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