The apoptotic effect of cordycepin on human OEC-M1 oral cancer cell line

Wei Ciao Wu, Jenn Ren Hsiao, Yu Yan Lian, Chun Yu Lin, Bu Miin Huang

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Cordycepin (3′-deoxyadenosine), a pure compound of Cordyceps sinensis, has been illustrated with anti-tumor effects. In the present study, the apoptotic effect of cordycepin on OEC-M1, a human oral squamous cancer cell line, was investigated by morphological observations, cell viability assay, annexin V-FITC analysis and flow cytometry methods. Results demonstrated that the number of rounded-up cell increased as treatment duration of cordycepin (100 μM) increased from 3 to 48 h, and the plasma membrane blebbing could be observed after 12 h treatment. In cell viability assay, cell surviving rate significantly decreased as the dosage and duration of cordycepin treatment increased (P < 0.05). Moreover, phosphatidylserine flipping on cell membrane could be detected with 3, 6 and 12 h cordycepin treatment, which indicated an early apoptotic phenomenon. Furthermore, cell cycle studies illustrated that the percentage of G1 phase cell declined as the dosages of cordycepin increased (10 μM to 5 mM), while the percentages of G2M and subG1 phase cell increased (P < 0.05) in 12, 24 and 48 h cordycepin treatment. These results further confirmed the apoptotic event. In conclusion, cordycepin significantly induced cell apoptotsis in OEC-M1 human oral squamous cancer cells.

Original languageEnglish
Pages (from-to)103-111
Number of pages9
JournalCancer Chemotherapy and Pharmacology
Volume60
Issue number1
DOIs
Publication statusPublished - Jun 1 2007
Externally publishedYes

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Keywords

  • Apoptosis
  • Cell cycle
  • Cell viability
  • Cordycepin
  • OEC-M1
  • Oral cancer

ASJC Scopus subject areas

  • Oncology
  • Toxicology
  • Pharmacology
  • Cancer Research
  • Pharmacology (medical)

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