Abstract

The anticancer effects of ceramide have been reported in many types of cancers but less in lung cancer. In this study, we used C2-ceramide to further investigate its possible anticancer effects and mechanisms on non-small cell lung cancer (NSCLC) H1299 cells. The result of cell proliferation in terms of trypan blue assay showed high dose of C2-ceramide inhibited cell survival after 24 h treatment. The flow cytometry-based assays indicated the effect of apoptosis, chromatin condensation, and G1 arrest in terms of Annexin V/propidium iodide (PI), DAPI, and PI stainings, respectively. Moreover, the decreased protein level of p-Akt, p-NFκB, survivin and cyclin A2 were detected by Western blot assay. Taken together, these results indicated the antiproliferative effect of C2-ceramide is majorly responsible for cell apoptosis in lung cancer H1299 cells.

Original languageEnglish
Article number1
JournalCancer Cell International
Volume14
Issue number1
DOIs
Publication statusPublished - Jan 6 2014

Keywords

  • Apoptosis
  • Ceramides
  • cyclin A2
  • Lung cancer
  • NSCLC
  • p-Akt
  • p-NFκB
  • survivin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Genetics

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