The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB

I. Ling Lin, Han Lin Chou, Jin Ching Lee, Feng Wei Chen, Yao Fong, Wei Chiao Chang, Hurng W. Huang, Chang Yi Wu, Wen Tsan Chang, Hui Min D Wang, Chien Chih Chiu

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13 Citations (Scopus)

Abstract

The anticancer effects of ceramide have been reported in many types of cancers but less in lung cancer. In this study, we used C2-ceramide to further investigate its possible anticancer effects and mechanisms on non-small cell lung cancer (NSCLC) H1299 cells. The result of cell proliferation in terms of trypan blue assay showed high dose of C2-ceramide inhibited cell survival after 24 h treatment. The flow cytometry-based assays indicated the effect of apoptosis, chromatin condensation, and G1 arrest in terms of Annexin V/propidium iodide (PI), DAPI, and PI stainings, respectively. Moreover, the decreased protein level of p-Akt, p-NFκB, survivin and cyclin A2 were detected by Western blot assay. Taken together, these results indicated the antiproliferative effect of C2-ceramide is majorly responsible for cell apoptosis in lung cancer H1299 cells.

Original languageEnglish
Article number1
JournalCancer Cell International
Volume14
Issue number1
DOIs
Publication statusPublished - Jan 6 2014

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Keywords

  • Apoptosis
  • Ceramides
  • cyclin A2
  • Lung cancer
  • NSCLC
  • p-Akt
  • p-NFκB
  • survivin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Genetics

Cite this

Lin, I. L., Chou, H. L., Lee, J. C., Chen, F. W., Fong, Y., Chang, W. C., Huang, H. W., Wu, C. Y., Chang, W. T., Wang, H. M. D., & Chiu, C. C. (2014). The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB. Cancer Cell International, 14(1), [1]. https://doi.org/10.1186/1475-2867-14-1