The antiplatelet activity of tetramethylpyrazine is mediated through activation of NO synthase

Joen Rong Sheu, Ya Chen Kan, Wei Chun Hung, Chien-Huang Lin, Mao Hsiung Yen

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)

Abstract

Tetramethylpyrazine (TMPZ) is an active ingredient of a Chinese herbal medicine (Ligusticum wallichii Franchat). In this study, TMPZ (50-200 μM) significantly increased production of nitrate and cyclic GMP in human platelets within a 15-min incubation period. TMPZ concentration-dependently inhibited intracellular Ca2+ mobilization in human platelets stimulated by collagen (5 μg/ml). Furthermore, TMPZ concentration (50 and 200 μM)- and time (15 and 30 min)-dependently triggered endothelial-type constitutive nitric oxide synthase (ecNOS) protein expression in human platelets. These results indicated that TMPZ at micromolar concentrations stimulated nitric oxide production in human platelets via a novel mechanism that activated ecNOS protein expression. (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)937-947
Number of pages11
JournalLife Sciences
Volume67
Issue number8
DOIs
Publication statusPublished - Jul 14 2000

Keywords

  • Cyclic GMP
  • ecNOS
  • Nitric oxide
  • Platelet
  • Tetramethylpyrazine

ASJC Scopus subject areas

  • Pharmacology

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