The anti-hepatic fibrosis activity of ergosterol depended on upregulation of PPARgamma in HSC-T6 cells

Chen Jei Tai, Chen Yen Choong, Yu Chun Lin, Yeu Ching Shi, Cheng Jeng Tai

Research output: Contribution to journalArticle

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Abstract

Advanced glycation endproducts (AGEs) were shown to play an important role in metabolic syndrome and were suggested to contribute to the development of hepatic fibrosis. Evidence indicates that AGEs resulted in hepatic fibrosis coupled to the activation of the receptor for AGEs (RAGE) in hepatic stellate cells (HSCs). NADPH oxidase is downstream of the RAGE signaling pathway, resulting in an increase in reactive oxygen species (ROS), alpha-smooth muscle actin (alpha-SMA), RAGE, and matrix metalloproteinase-9 (MMP-9). This study was designed to evaluate the effects of ergosterol on RAGE signaling in HSC-T6 cells. Ergosterol suppressed the activation of HSC-T6 cells induced by AGEs, and attenuated overexpressions of alpha-SMA, MMP-9, and epithelial-mesenchymal transition (EMT) markers, including N-cadherin and vimentin. We also found that these inhibitory effects of ergosterol on the activation of HSCs were dependent on peroxisome proliferator-activated receptor-gamma (PPARgamma) confirmed by PPARgamma reporter assay and PPARgamma knockdown. In addition, ergosterol also showed an inhibitory effect on the generation of AGEs, fructosamine, and α-dicarbonyl compounds in this study. Our results show that ergosterol can be used as a protective agent against hepatic fibrosis caused by induction of AGEs.

Original languageEnglish
Pages (from-to)1915-1923
Number of pages9
JournalFood and Function
Volume7
Issue number4
DOIs
Publication statusPublished - Apr 1 2016

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Ergosterol
Hepatic Stellate Cells
liver cirrhosis
ergosterol
PPAR gamma
Fibrosis
Up-Regulation
liver
Liver
Matrix Metalloproteinase 9
receptors
gelatinase B
Smooth Muscle
cells
Actins
smooth muscle
actin
Fructosamine
Protective Agents
Cohort Effect

ASJC Scopus subject areas

  • Food Science

Cite this

The anti-hepatic fibrosis activity of ergosterol depended on upregulation of PPARgamma in HSC-T6 cells. / Tai, Chen Jei; Choong, Chen Yen; Lin, Yu Chun; Shi, Yeu Ching; Tai, Cheng Jeng.

In: Food and Function, Vol. 7, No. 4, 01.04.2016, p. 1915-1923.

Research output: Contribution to journalArticle

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