The 5' untranslated regions of the rat A(2A) adenosine receptor gene function as negative translational regulators

Yi Chao Lee, Chiung Wen Chang, Cheng Wen Su, Teng Nan Lin, Synthia H. Sun, Hsin Lin Lai, Yijuang Chern

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The rat A(2A) adenosine receptor (A(2A)-R) gene contains two promoters, P1 and P2, which produce transcript I and transcript 2, respectively. These transcripts differ in the lengths of their 5' untranslated regions (5'UTR1: 514 bp, initiated from P1; 5'UTR2: 221 bp, initiated from P2) but encode the same protein. In the present study, we demonstrate that transcript 2 is present in various tissues at different levels, whereas transcript I is found only in the striatum. In the striatum, the level of transcript 2 is ~300- fold higher than that of transcript 1. The 5'UTR of both transcripts suppresses the expression of A(2A)-R and a firefly luciferase reporter gene at the translational level; this suppression is not observed after mutational inactivation of an 'out-of-frame' upstream AUG codon. Translational suppression by the 5'UTR was also confirmed in cells using a bicistronic strategy. Collectively, these data suggest that P2 is the major promoter of the rat A(2A)-R gene. The 5'UTR of the rat A(2A)-R gene exerts an inhibitory effect on translation by an upstream open reading frame. Because the 5'UTR of the A(2A)-R gene possesses strong interspecies homology, translational suppression may be a general mechanism by which the expression of the A(2A)-R gene is regulated.

Original languageEnglish
Pages (from-to)1790-1798
Number of pages9
JournalJournal of Neurochemistry
Volume73
Issue number5
DOIs
Publication statusPublished - 1999
Externally publishedYes

Keywords

  • A(2A) adenosine receptor
  • Gene expression
  • Multiple promoters
  • Striatum
  • Translation

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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