The (-1486T/C) promoter polymorphism of the TLR-9 gene is associated with end-stage knee osteoarthritis in a Chinese population

Sui Lung Su, Hsin Yi Yang, Chian Her Lee, Guo Shu Huang, Donald M. Salter, Herng Sheng Lee

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Based on the recent observation that Toll-like receptors (TLRs) may be involved in the pathogenesis of osteoarthritis (OA) we explored the possibility that human TLR gene polymorphisms are associated with OA. Two separate populations were studied in a two-stage case-control study with a total of 503 OA patients and 428 healthy controls. The TLR-2, TLR-4, and TLR-9 genotypes were assessed by real-time polymerase chain reaction. Our data demonstrated a lack of association among TLR-2, TLR-4, and TLR-9 (T-1237C) polymorphisms and the risk of developing OA in both stages of the study. T-1486C was significantly associated with OA in both populations with G1635A of TLR-9 gene was found to be significantly associated with OA when the two populations were combined. Stratifying the samples by K-L score there were significant differences in the genotype of the TLR-9 T-1486C and G1635A between OA of the knee grade 4 and controls. In haplotype analyses, the haplotype TTG and TTA revealed higher risk of OA and TCA confers a lower risk of OA in combined population. The present results demonstrate that TLR-9 polymorphisms, in particular T-1486C is significantly associated with OA. TLR-9 gene polymorphisms may play a role in the etiology of knee OA. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:9-14, 2012

Original languageEnglish
Pages (from-to)9-14
Number of pages6
JournalJournal of Orthopaedic Research
Volume30
Issue number1
DOIs
Publication statusPublished - Jan 2012

Fingerprint

Toll-Like Receptor 9
Knee Osteoarthritis
Osteoarthritis
Population
Genes
Toll-Like Receptor 2
Toll-Like Receptor 4
Toll-Like Receptors
Haplotypes
Genotype
Case-Control Studies
Real-Time Polymerase Chain Reaction

Keywords

  • osteoarthritis
  • single nucleotide polymorphism
  • Toll-like receptor

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

The (-1486T/C) promoter polymorphism of the TLR-9 gene is associated with end-stage knee osteoarthritis in a Chinese population. / Su, Sui Lung; Yang, Hsin Yi; Lee, Chian Her; Huang, Guo Shu; Salter, Donald M.; Lee, Herng Sheng.

In: Journal of Orthopaedic Research, Vol. 30, No. 1, 01.2012, p. 9-14.

Research output: Contribution to journalArticle

Su, Sui Lung ; Yang, Hsin Yi ; Lee, Chian Her ; Huang, Guo Shu ; Salter, Donald M. ; Lee, Herng Sheng. / The (-1486T/C) promoter polymorphism of the TLR-9 gene is associated with end-stage knee osteoarthritis in a Chinese population. In: Journal of Orthopaedic Research. 2012 ; Vol. 30, No. 1. pp. 9-14.
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