Thallium acetate induces C6 glioma cell apoptosis

Chee Fah Chia, Soul-Chin Chen, Chin Shyang Chen, Chuen Ming Shih, Horng Mo Lee, Chih Hsiung Wu

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16 Citations (Scopus)

Abstract

Thallium acetate is a known neurotoxic agent. In this study, we investigated the mechanisms by which thallium acetate induces cell cycle arrest and cell apoptosis in cultured LC6 glioma cells. Exposure of C6 glioma cells to thallium acetate decreased cell viability as demonstrated by the MTT assay. Incubation of thallium acetate arrested cell cycle progression at the G 2/M phase and caused cellular apoptosis at 300 μM as determined by trypan blue exclusion and flow cytometric analysis. The G2/M arrest was associated with a decrease in expression of CDK2 protein and an upregulation of p53 and the CDK inhibitor p21Cip1, but not p27Kip1. Thallium acetate did not alter the protein levels of cyclin A and B; cyclin D1, D2, and D3; and CDK4 expression in C6 glioma cells. Incubation of C6 glioma cells with thallium acetate upregulated the expression of proapoptotic proteins Bad and Apaf and downregulated the expression of anti-apoptotic proteins Bcl-xL and Bcl-2. In conclusion, these data suggest that thallium acetate inhibits cell cycle progression at G2/M phase by suppressing CDK activity through the p53-mediated induction of the CDK inhibitor p21Cip1. Impairment of cell cycle progression may trigger the activation of a mitochondrial pathway and shifts the balance in the Bcl-2 family toward the proapoptotic members, promoting the formation of the apoptosome and, consequently, apoptosis.

Original languageEnglish
Pages (from-to)523-530
Number of pages8
JournalAnnals of the New York Academy of Sciences
Volume1042
DOIs
Publication statusPublished - 2005

Keywords

  • C6 glioma cells
  • GM arrest
  • Mitochondria
  • p21
  • p27
  • Thallium acetate

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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  • Cite this

    Chia, C. F., Chen, S-C., Chen, C. S., Shih, C. M., Lee, H. M., & Wu, C. H. (2005). Thallium acetate induces C6 glioma cell apoptosis. Annals of the New York Academy of Sciences, 1042, 523-530. https://doi.org/10.1196/annals.1338.064