Tetramethylpyrazine as potassium channel opener to lower calcium influx into cultured aortic smooth muscle cells

Chin Chuan Tsai, Tung-Iuan Lai, Wei Chan Huang, Tzyylin Yang, I. Min Liu, Kar Lok Wong, Paul Chan, Juei Tang Cheng

Research output: Contribution to journalArticle

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Abstract

In the present study, the effect of tetramethylpyrazine (TMP) on calcium (Ca2+) influx was investigated in cultured vascular smooth muscle (A7r5) cells using Fura-2 as an indicator. The increase of Ca2+ concentration in A7r5 cells produced by vasopressin or phenylephrine was attenuated by TMP from 0.01 μmol/L to 1 mmol/L. The decrease in the intracellular potassium concentration in A7r5 cells by TMP from 0.01 μmol/L to 10 μmol/L was characterized using PBFI/AM. Inhibitors specific to the small conductance calcium-activated potassium (SKCa) channel or the ATP-sensitive potassium (KATP) channel abolished the actions of TMP. The obtained results indicate that the decrease of Ca2+ influx into A7r5 cells by TMP is mainly mediated by the opening of potassium channels.

Original languageEnglish
Pages (from-to)557-558
Number of pages2
JournalPlanta Medica
Volume69
Issue number6
DOIs
Publication statusPublished - Jun 1 2003

Fingerprint

Potassium Channels
potassium channels
smooth muscle
myocytes
Smooth Muscle Myocytes
Muscle
Cells
Calcium
calcium
KATP Channels
Small-Conductance Calcium-Activated Potassium Channels
Potassium
phenylephrine
vasopressin
Fura-2
Phenylephrine
cells
Vasopressins
Vascular Smooth Muscle
blood vessels

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Tsai, C. C., Lai, T-I., Huang, W. C., Yang, T., Liu, I. M., Wong, K. L., ... Cheng, J. T. (2003). Tetramethylpyrazine as potassium channel opener to lower calcium influx into cultured aortic smooth muscle cells. Planta Medica, 69(6), 557-558. https://doi.org/10.1055/s-2003-40638

Tetramethylpyrazine as potassium channel opener to lower calcium influx into cultured aortic smooth muscle cells. / Tsai, Chin Chuan; Lai, Tung-Iuan; Huang, Wei Chan; Yang, Tzyylin; Liu, I. Min; Wong, Kar Lok; Chan, Paul; Cheng, Juei Tang.

In: Planta Medica, Vol. 69, No. 6, 01.06.2003, p. 557-558.

Research output: Contribution to journalArticle

Tsai, Chin Chuan ; Lai, Tung-Iuan ; Huang, Wei Chan ; Yang, Tzyylin ; Liu, I. Min ; Wong, Kar Lok ; Chan, Paul ; Cheng, Juei Tang. / Tetramethylpyrazine as potassium channel opener to lower calcium influx into cultured aortic smooth muscle cells. In: Planta Medica. 2003 ; Vol. 69, No. 6. pp. 557-558.
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